Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,380,367	C→T	D51N (GAC→AAC)	frdB ←	fumarate reductase iron‑sulfur protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,380,367	0	C	T	100.0%	182.0 / NA	52	D51N (GAC→AAC)	frdB	fumarate reductase iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/27);  total (25/27)

AGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGG  >  NC_000913/4380122‑4380596                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      aGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTg                                                                                                                                                                                                                                  >  2:225312/1‑251 (MQ=255)      ccTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAAGCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAgg                                                                                                                                                                                                                             <  2:141471/251‑1 (MQ=255)         gATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGt                                                                                                                                                                                                                            <  1:66288/249‑1 (MQ=255)          aTCCGGGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGt                                                                                                                                                                                                                         <  1:353734/251‑1 (MQ=255)                      ggAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGc                                                                                                                                                                                                             >  1:84254/1‑251 (MQ=255)                             ccGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGGGCCAGGTTGTCTTTGATGTAGccccacgc                                                                                                                                                                                                      >  2:83639/1‑246 (MQ=255)                               gatgatGTAAGGTTTGATCGCTTACAGACTTTCGATGCAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGACGTTAGATAACGCTTCAACCTTCATCCCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATAATGCAGCAGGAACCACAAATCGACATACGGCAGGACCAGCGGTAGCTCAGGTTTGGTGACAGGTTGTCTTTGATGTAGCCCAGCGCAt                                                                                                                                                                                                    >  2:153093/1‑251 (MQ=255)                                    tgTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGt                                                                                                                                                                                               >  1:321896/1‑251 (MQ=255)                                        cGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATg                                                                                                                                                                                           >  1:134216/1‑251 (MQ=255)                                        cGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATg                                                                                                                                                                                           <  2:328081/251‑1 (MQ=255)                                          gTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAg                                                                                                                                                                                         <  1:259112/251‑1 (MQ=255)                                              gATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTa                                                                                                                                                                                      <  1:260947/250‑1 (MQ=255)                                              gATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGGTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTa                                                                                                                                                                                      <  2:297230/250‑1 (MQ=255)                                                          aCTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCCACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATaa                                                                                                                                                                          >  2:347199/1‑250 (MQ=255)                                                               cGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACt                                                                                                                                                                    >  1:238943/1‑251 (MQ=255)                                                               cGATGAAGTGGGTAATATCGACCACCAGAGCGCGTTCAATCGGGAATTTAGCGAACGCTTCAACCTTCATACCGTCTGTGTAATCACGCAGGAAGGTTTTACATGCCAGGTTTGGCACTTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCAGACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTGTGATGTAGCCCGTCGCATCCAGTAATGAGGTAGTTTCATCATAAGGCAc                                                                                                                                                                     <  2:201195/250‑1 (MQ=255)                                                                    aaGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATa                                                                                                                                                               <  1:42810/251‑1 (MQ=255)                                                                                 cGACCACCAGATCGCGTTCCATCGGGAAGTTAGCTACAGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGATTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCCCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGGAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtgc                                                                                                                                                  >  2:125408/1‑251 (MQ=255)                                                                                     caccaGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtgcggtg                                                                                                                                              >  1:293800/1‑251 (MQ=255)                                                                                       ccaGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtgcggtgcg                                                                                                                                            <  2:275267/251‑1 (MQ=255)                                                                                         aGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATgcggtgcggt                                                                                                                                          <  1:179111/251‑1 (MQ=255)                                                                                                            aGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAg                                                                                                                       <  1:275434/251‑1 (MQ=255)                                                                                                             gTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAgc                                                                                                                      <  2:355218/251‑1 (MQ=255)                                                                                                                aGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTT‑GATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGcac                                                                                                                  <  2:316816/251‑1 (MQ=255)                                                                                                                     aCGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCaccacc                                                                                                              >  1:148777/1‑251 (MQ=255)                                                                                                                      cGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCt                                                                                                             <  1:225312/251‑1 (MQ=255)                                                                                                                          tCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCaa                                                                                                          <  1:76791/250‑1 (MQ=255)                                                                                                                           cAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAAt                                                                                                         <  1:246462/250‑1 (MQ=255)                                                                                                                           cAACCTTCATAACGTAGGTGAAATCACGCAGGAAGGTTTTACATGCCAGTACTGGCACGTTGATAACCCTCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCCGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATAGACTTCCGGGTTATAGCGCACCACCTCAAtt                                                                                                        >  2:31222/1‑251 (MQ=255)                                                                                                                             aCCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAAtttt                                                                                                      <  2:27636/251‑1 (MQ=255)                                                                                                                                   aaaCCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATAGCCATACGGCAGGCACAGCGGTAGCTCAGGTTCGGGACCCGGTTGTCTATGATGTAGCCCAGCGCCTCCAGTAATGAGGATGTTGCGTCATAAGGAACTGCATAGAAAGCGCTATGCGGTGAGGTATCGTCTTCCGGGTAAAAGCGCAACACCTTAATTTTTAGGtt                                                                                                >  2:18765/3‑251 (MQ=255)                                                                                                                                     aCCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGttt                                                                                               <  2:285088/250‑1 (MQ=255)                                                                                                                                      ccGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGttttt                                                                                             <  1:279337/251‑1 (MQ=255)                                                                                                                                            gtgtAATCACGCCGGAAGGTTTTACATGCCCGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCCCCACTCGCCATACGGCAGGACCAGCGGTAGCTCCGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtctc                                                                                       >  2:218278/1‑251 (MQ=255)                                                                                                                                             tgtAATCACGCAGGAAGGATTTACATGCCAGTTTTGGCACGTTGTTACCCATCATGCCGCCGGACCCCCAAATCGCCATACGGCAGGACCAACCGTAGCTCAGGTTCGGTGCCAGGATGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCAtatc                                                                                       >  1:359215/1‑247 (MQ=255)                                                                                                                                                        aGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGcct                                                                           >  1:30604/1‑251 (MQ=255)                                                                                                                                                              gTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctcct                                                                     <  2:238943/251‑1 (MQ=255)                                                                                                                                                                  tACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGccttctccttct                                                                  <  1:218278/250‑1 (MQ=255)                                                                                                                                                                      tGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTg                                                             >  1:318827/1‑251 (MQ=255)                                                                                                                                                                                        gttAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCAGCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCg                                           <  2:350719/251‑1 (MQ=255)                                                                                                                                                                                                tcatGCCGCAGGAACCACCAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCt                                                                                                             >  2:224993/1‑177 (MQ=255)                                                                                                                                                                                                tcatGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGGTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCt                                                                                                             <  1:224993/177‑1 (MQ=255)                                                                                                                                                                                                     ccGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtg                                                                                                                                                   <  2:87946/134‑1 (MQ=255)                                                                                                                                                                                                     ccGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTAtg                                                                                                                                                   >  1:87946/1‑134 (MQ=255)                                                                                                                                                                                                     ccGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCtt                              >  1:258677/1‑251 (MQ=255)                                                                                                                                                                                                      cGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTc                             >  1:65304/1‑251 (MQ=255)                                                                                                                                                                                                        cAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGc                           >  2:91760/1‑251 (MQ=255)                                                                                                                                                                                                         aGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGct                          >  2:242781/1‑250 (MQ=255)                                                                                                                                                                                                           gAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCCGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGGATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCAc                        >  2:34044/1‑251 (MQ=255)                                                                                                                                                                                                                      cGCCATACGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGt             >  1:126719/1‑251 (MQ=255)                                                                                                                                                                                                                            aCGGCAGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCt       <  1:264409/251‑1 (MQ=255)                                                                                                                                                                                                                                 aGGACCAGCGGTAGCTCAGGTTCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTggcgg  <  2:258677/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                      AGTACCCTGATCCGCGGTGCGGGAGTTGCCGATGATGTACGGTTTGATCGCTTCCAGACTTTCGATGAAGTGGGTCATATCGACCACCAGATCGCGTTCAATCGGGAAGTTAGCTAACGCTTCAACCTTCATACCGTCGGTGTAATCACGCAGGAAGGTTTTACATGCCAGTTTTGGCACGTTGTTAACCATCATGCCGCAGGAACCACAAATCGCCATACGGCAGGACCAGCGGTAGCTCAGGTCCGGTGCCAGGTTGTCTTTGATGTAGCCCAGCGCATCCAGTAATGAGGTAGTTGCGTCATAAGGCACTTCATAGAATGCGCTATGCGGTGCGGTATCGACTTCCGGGTTATAGCGCACCACCTCAATTTTCAGGTTTTTCATCTCAGCCATTCGCCTTCTCCTTCTTATTGGCTGCTTCCGCCTTATCGGCTGCATCCGCTTCGCCACCGTAAACGCGTTTAGCTGGCGG  >  NC_000913/4380122‑4380596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑