Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,337,390	C→T	K180K (AAG→AAA)	adiY ←	DNA‑binding transcriptional activator AdiY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,337,390	0	C	T	100.0%	123.6 / NA	35	K180K (AAG→AAA)	adiY	DNA‑binding transcriptional activator AdiY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/19);  total (16/19)

TCGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGC  >  NC_000913/4337152‑4337634                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     tCGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGa                                                                                                                                                                                                                                             <  1:230640/248‑1 (MQ=255)  cGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTg                                                                                                                                                                                                                                         <  1:266930/251‑1 (MQ=255)             aaaaTCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATCCCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACAt                                                                                                                                                                                                                              >  2:143723/1‑251 (MQ=255)                     gCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACt                                                                                                                                                                                                                      >  1:350555/1‑251 (MQ=255)                             gCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTAcc                                                                                                                                                                                                               >  1:8940/1‑250 (MQ=255)                                      gtgCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTATGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAg                                                                                                                                                                                                       <  2:133194/249‑1 (MQ=255)                                       tgCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGAtt                                                                                                                                                                                                    >  1:53372/1‑251 (MQ=255)                                          tGACTAACATACTGCAGCGGAGTCATACCGTAGAAGTCTTTCAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTcc                                                                                                                                                                                                  >  2:211111/1‑250 (MQ=255)                                           gACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTcc                                                                                                                                                                                                  <  2:262164/249‑1 (MQ=255)                                           gACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCa                                                                                                                                                                                                 <  1:79933/250‑1 (MQ=255)                                           gACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAg                                                                                                                                                                                                <  2:48520/251‑1 (MQ=255)                                                               gTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGCTGTCGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCCTTAATTCATTTACGGCATAACGCATGCGGCCGGTGGTGATTATTTTGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTc                                                                                                                                                                            >  2:212697/1‑251 (MQ=255)                                                                  aTACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCaat                                                                                                                                                                         <  1:164641/251‑1 (MQ=255)                                                                    aCCGGAGAAGTCGTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGAGATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTGTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCaata                                                                                                                                                                        <  1:206386/250‑1 (MQ=255)                                                                           aaGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGAt                                                                                                                                                                 >  2:233137/1‑250 (MQ=255)                                                                            aGTCTTTAAAGACAGAAATAAAGTACGACTTACTGTTGTAGCCGCTGGACTGTGATACCTGAGAGATATGTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAATCAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTAGATaa                                                                                                                                                               <  2:103385/251‑1 (MQ=255)                                                                              tCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAAc                                                                                                                                                             >  1:282340/1‑251 (MQ=255)                                                                                tttAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGc                                                                                                                                                           >  2:5108/1‑251 (MQ=255)                                                                                     aGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTTTAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTAAATATCGCTTTCAATAATTTGATAAACGCTGTc                                                                                                                                                       >  2:248812/1‑250 (MQ=255)                                                                                      gACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGCTACCTGAGAGATATTTTTACCGTCCCTCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATCTCGCTTTCAATAATTTGATAAACGCTGTCAc                                                                                                                                                     >  1:219349/1‑251 (MQ=255)                                                                                                      gacgTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTaa                                                                                                                                     <  1:285626/251‑1 (MQ=255)                                                                                                                   aGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCAtt                                                                                                                        <  2:8940/251‑1 (MQ=255)                                                                                                                               gttaTACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGaa                                                                                                             <  2:215737/247‑1 (MQ=255)                                                                                                                                        gagagaTATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTg                                                                                                   <  1:279270/251‑1 (MQ=255)                                                                                                                                          gagaTATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTGTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTTCACAATTACTTAACATATGCATCATTAATGAAACGAATTTTTTACTGTc                                                                                                 <  1:331210/251‑1 (MQ=255)                                                                                                                                                                   aTTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGaat                                                                        <  1:211111/251‑1 (MQ=255)                                                                                                                                                                                     gcctGCGGCAGGCGCTGATTATTTGGCTATAACTGGTGTTTTAGCTTCTCAACTTTTTTTTTCACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTAAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTgttcgt                                                      >  2:219214/4‑251 (MQ=255)                                                                                                                                                                                         gCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCt                                                  <  2:75802/251‑1 (MQ=255)                                                                                                                                                                                               ggtggtGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTg                                            >  2:29464/1‑251 (MQ=255)                                                                                                                                                                                                          tttGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATg                                                                                                                    >  2:180743/1‑168 (MQ=255)                                                                                                                                                                                                          tttGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATg                                                                                                                    <  1:180743/168‑1 (MQ=255)                                                                                                                                                                                                                 ataACTGGTGTTTTCGCTTTTCAACTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGc                           <  1:249499/250‑1 (MQ=255)                                                                                                                                                                                                                                    ctCAACTTTTTTTACAACAGACTTGGCCCAAGACATCAACCACTCGCCAACATACTACGATCTCAGGCTTTGTGACTAGCGCTTCAAATCATTTGATAAACGCTTTAACTTCCCCAATTACGTAACATATACATAATTAATGAAACGATTTTTCTACTGTCGAGCAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTCCATTTTCTGACCCAGATTCTGCGGGCATCATGCTGTGTTgcgctgc       >  2:336305/2‑248 (MQ=255)                                                                                                                                                                                                                                      cAACTTTTTTTTTAACAGACGTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGATAAAACGGGATAGCGCCGTGAATAATAATGCGCGTGTGCGTTCCATTGCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCaa     <  1:9819/251‑1 (MQ=255)                                                                                                                                                                                                                                         cTTTTTTTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAAcgc  >  1:159624/1‑251 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                     TCGTTAAGGTTAAAAATCAGGCGACAGTGCGTTCTCTGTGCTGACTAACATAATGCAGCGGCGTCATACCGTAGAAGTCTTTAAAGACAGAAATAAAGTACGACGTACTGTTGTAGCCGCAGGACTGTGATACCTGAGAGATATTTTTACCGTCCATCATTAATTCATTTACGGCATAACGCATGCGGCAGGTGGTGATTATTTGGCTATAACTGGTGTTTTCGCTTTTCAACTTTTTCTTTAACAGACTTGGGCTAAGACATAAACAACTGGCTACCATACTAAGATTCCAGTCTTTGTGAATATCGCTTTCAATAATTTGATAAACGCTGTCACTTACACAATTACGTAACATATACATCATTAATGAAACGAATTTTTTACTGTCGAGAAAACGGGATAGCACCGTGAATAATAATGCGCGTGTTCGTTCCTTTTCTGACTCAGTTTCTGCGGGCATCATGCTGTGTTGCGCGGCAACGC  >  NC_000913/4337152‑4337634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑