Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,536	G→A	Y528Y (TAC→TAT)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,536	0	G	A	100.0%	209.4 / NA	58	Y528Y (TAC→TAT)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/32);  total (26/32)

ATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTT  >  NC_000913/3878291‑3878781                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ataAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGAc                                                                                                                                                                                                                                                  >  2:107717/1‑251 (MQ=255) ataAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGAGGCTGAGCGATATAGAc                                                                                                                                                                                                                                                  <  2:194044/251‑1 (MQ=255)          ttCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACcg                                                                                                                                                                                                                                           <  1:189279/249‑1 (MQ=255)          ttCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGGCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACcgc                                                                                                                                                                                                                                          <  2:292404/250‑1 (MQ=255)           tCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGt                                                                                                                                                                                                                                        >  1:213208/1‑251 (MQ=255)              gCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCa                                                                                                                                                                                                                                     <  2:203649/251‑1 (MQ=255)              gCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCa                                                                                                                                                                                                                                     <  2:209456/251‑1 (MQ=255)                  tGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACg                                                                                                                                                                                                                                  <  2:265560/250‑1 (MQ=255)                           aTAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGc                                                                                                                                                                                                                         >  1:121073/1‑250 (MQ=255)                            tAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCAt                                                                                                                                                                                                                       >  1:280074/1‑251 (MQ=255)                             aaCGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCAt                                                                                                                                                                                                                       <  2:196635/250‑1 (MQ=255)                                 acgCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGAc                                                                                                                                                                                                                  >  2:315725/1‑251 (MQ=255)                                  cgCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACg                                                                                                                                                                                                                 >  1:31331/1‑251 (MQ=255)                                                         cTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCg                                                                                                                                                                                          >  1:134753/1‑251 (MQ=255)                                                                 cgTTGTACTCAGGTACCAGGTTCTCTAACGCCTACCCAGCCCATGCCGGTGCACTGGCGTTGGAGTGCCGCGTTGCGCCGTCCAGCGCGATAGAGCTCTGGTACTGATCCATCGCTTCGTCGTATTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCAGAGCGACATAGACGGGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAAAAGCAGCGTACGAAtg                                                                                                                                                                                  >  2:27286/1‑251 (MQ=255)                                                                            gATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTc                                                                                                                                                                       >  1:256394/1‑251 (MQ=255)                                                                             aTACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCg                                                                                                                                                                      >  1:213018/1‑251 (MQ=255)                                                                             aTACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCg                                                                                                                                                                      <  2:356457/251‑1 (MQ=255)                                                                               aCCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGAc                                                                                                                                                                    <  1:47122/251‑1 (MQ=255)                                                                                       ctctAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCAt                                                                                                                                                            >  2:77678/1‑251 (MQ=255)                                                                                            ccGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTc                                                                                                                                                       <  1:41783/250‑1 (MQ=255)                                                                                            aCGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTc                                                                                                                                                       >  2:268724/1‑251 (MQ=255)                                                                                              gCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGAGTTGCGCCGTCAAGCGCGATAGAGATCTGGTACTGATCCATAGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGAAGATAGAAGAAGGTCAACAGAAGCGTACGAATGTGCGAGCCGTCGACGTCGGCATCGGTCat                                                                                                                                                     >  1:21261/1‑251 (MQ=255)                                                                                               cTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCACCAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCCTCGGTCatg                                                                                                                                                    >  1:242590/1‑251 (MQ=255)                                                                                                 tCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCatg                                                                                                                                                    <  1:77436/249‑1 (MQ=255)                                                                                                           aTGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCACCGTACGAATGTGCGGGCCGGCGACGTCCGCATTGGTCATGATGATGATGCtg                                                                                                                                        <  2:178411/251‑1 (MQ=255)                                                                                                                        ggCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGt                                                                                                                            <  2:213208/250‑1 (MQ=255)                                                                                                                         gCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGCGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGttt                                                                                                                          >  2:89715/1‑251 (MQ=255)                                                                                                                          cGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTg                                                                                                                         <  1:281313/251‑1 (MQ=255)                                                                                                                           gTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGt                                                                                                                        <  2:190197/251‑1 (MQ=255)                                                                                                                                     aGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTa                                                                                                              <  2:229047/251‑1 (MQ=255)                                                                                                                                        gTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTc                                                                                                           >  1:247448/1‑251 (MQ=255)                                                                                                                                          ggcgcCGTCCAGCGCGATAGAGATCTGGTACTGATCCAGCGCTTCTGCGTCTTTAATGTACTGTGCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCg                                                                                                          <  2:352170/249‑1 (MQ=255)                                                                                                                                              ccGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                      >  1:303963/1‑250 (MQ=255)                                                                                                                                              ccGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCAc                                                                                                      <  2:303963/250‑1 (MQ=255)                                                                                                                                              ccGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACg                                                                                                     >  2:342462/1‑251 (MQ=255)                                                                                                                                               cGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATA‑CGCAGTTTGTCCGGGTTGTACTCCTCACGAc                                                                                                   >  2:272138/1‑251 (MQ=255)                                                                                                                                                       gcgATAGAGATCTGGTACGGATCCATCGCTTCGGCGTCTGTAATGTACTGTTCCTGCTTGCCTTTCTTCACGTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATAcc                                                                                            <  2:361648/251‑1 (MQ=255)                                                                                                                                                              agaTCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCTTCACGACCGATACCACAGcc                                                                                      >  1:277487/1‑250 (MQ=255)                                                                                                                                                              agaTCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGcc                                                                                      >  1:353009/1‑250 (MQ=255)                                                                                                                                                                          gATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGAt                                                                          >  1:172881/1‑250 (MQ=255)                                                                                                                                                                                              ttAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTga                                                       <  2:110069/249‑1 (MQ=255)                                                                                                                                                                                                aaTGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGagaa                                                    <  1:269286/250‑1 (MQ=255)                                                                                                                                                                                                   gTACTGTTCCTGCTTGCCTTTCTGCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGGCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAagaga                                                <  1:347589/251‑1 (MQ=255)                                                                                                                                                                                                      cTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                              >  1:308966/1‑250 (MQ=255)                                                                                                                                                                                                      cTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGAAGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCCACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCCTGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGc                                              >  2:26680/1‑250 (MQ=255)                                                                                                                                                                                                           ccTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTa                                        <  1:304400/251‑1 (MQ=255)                                                                                                                                                                                                            cTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTAt                                       >  2:10808/1‑251 (MQ=255)                                                                                                                                                                                                              gCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATc                                      <  1:268724/250‑1 (MQ=255)                                                                                                                                                                                                                 tGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGaa                                   <  1:269202/250‑1 (MQ=255)                                                                                                                                                                                                                  gCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAg                                  <  2:173272/250‑1 (MQ=255)                                                                                                                                                                                                                          tcACTTTGTACAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCt                            <  1:123935/248‑1 (MQ=255)                                                                                                                                                                                                                                    cAGCGGCGGCTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa               <  1:218345/251‑1 (MQ=255)                                                                                                                                                                                                                                         gcggcTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAtt          <  1:123460/251‑1 (MQ=255)                                                                                                                                                                                                                                          cggcTGAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGAttt         >  1:172388/1‑251 (MQ=255)                                                                                                                                                                                                                                            gcggAGCGATATAGTCGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTa       <  2:333659/248‑1 (MQ=255)                                                                                                                                                                                                                                               gAGCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAcc     <  1:10808/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gCGATATAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCtt  <  2:312706/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ATAAGCTCTTTCAGCATTGCTTTCGGATAACGACGCTCCATACGATTGATCATTTTCTGCGTCGCGTTGTACTCAGATACCAGTTTCTCTAACGCTTCGCCAGCCAATGCCGGTGCACTGGCGTTGGTGTGCAGCGTTGCGCCGTCCAGCGCGATAGAGATCTGGTACTGATCCATCGCTTCGTCGTCTTTAATGTACTGTTCCTGCTTGCCTTTCTTCACTTTGTACAGCGGCGGCTGAGCGATGTAGACGTGACCGCGTTCAACGATTTCCGGCATCTGACGATAGAAGAAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTT  >  NC_000913/3878291‑3878781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑