Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,637	C→A	L87L (CTC→CTA)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,637	0	C	A	100.0%	168.0 / NA	46	L87L (CTC→CTA)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/18);  total (28/18)

CGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGA  >  NC_000913/3869391‑3869879                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAAcgc                                                                                                                                                                                                                                                >  1:74850/1‑251 (MQ=255) cGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAAcgc                                                                                                                                                                                                                                                >  1:19566/1‑251 (MQ=255)      cGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTCTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAAc                                                                                                                                                                                                                                           >  1:51098/1‑251 (MQ=255)                   cGGCCCGGTAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAAGGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGGGATGGGCTGACGCTTCCTGTCGATGTGATCGTTAATCCGCAGATTTTCAGCGTCAAAACGGTCGACGTCGCCGCATCGGTAACTCGTAACGGCCAGCGAAg                                                                                                                                                                                                                                <  2:168570/249‑1 (MQ=255)                       ccGGCAGGATCGGCGTTGGCCCGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTatat                                                                                                                                                                                                                          >  1:310071/1‑251 (MQ=255)                          gCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCaa                                                                                                                                                                                                                       <  1:199422/251‑1 (MQ=255)                                               aaGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCg                                                                                                                                                                                                   >  1:340522/1‑250 (MQ=255)                                                aGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCAACAGTGTGATACTGAAGGATTCAGCAAACCCTGCGGCGGTCTGCTAGCACCGGACGCGCAACGATCTTTTATTCGAGATGGGCTGACGCTTCCTGTCGATGAGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGACGACGCCGCAGCATCGCTAACGCGTCACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc                                                                                                                                                                                                 >  1:216990/1‑251 (MQ=255)                                                             aaTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCg                                                                                                                                                                                                                                   <  1:18380/204‑1 (MQ=255)                                                             aaTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCg                                                                                                                                                                                                                                   >  2:18380/1‑204 (MQ=255)                                                              aTGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATc                                                                                                                                                                                   <  2:6485/251‑1 (MQ=255)                                                                         cgcgCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCg                                                                                                                                                                        >  2:242386/1‑251 (MQ=255)                                                                         cgagcTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATGCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATAGCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATcccc                                                                                                                                                                         <  2:233707/247‑1 (MQ=255)                                                                               ggATAAAAAGCACCAGTTTGGTACGGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCTATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCTCCTCATCGTTATCGTTTAACTACCAGCGAAGCTATATCAATATTAATCGCTATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAg                                                                                                                                                                    <  1:36435/249‑1 (MQ=255)                                                                                gATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAAACCTGCGGCGGTCTGCTGGCACCGGACGAGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGt                                                                                                                                                                 >  1:23506/1‑251 (MQ=255)                                                                                                                    aaaCCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGGCGAAAAATCTg                                                                                                                             <  1:283073/251‑1 (MQ=255)                                                                                                                           gcggcgGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGAGGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCATCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTTGCGTg                                                                                                                        <  1:242386/249‑1 (MQ=255)                                                                                                                            cggggtcctgctgTCCCCGGCCGCGCAGCGTTCTTTTATTCCCGTTGGCCTGACGCTTCCTATCGATTTTCTCGCTTCTCCGCTGATTTTCTGCGCCAATCCCTTCTACGTCGCCGCATCGCTATCGCGTAACTCCCCGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCGGTTCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAgg                                                                                                                     <  1:271769/244‑1 (MQ=255)                                                                                                                             ggcggTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGga                                                                                                                    <  1:135096/251‑1 (MQ=255)                                                                                                                                      ctgGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATg                                                                                                           >  2:326185/1‑251 (MQ=255)                                                                                                                                        ggCACCGGGCGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCACCGTCAAAACCGTCGACTTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGc                                                                                                         <  1:161050/251‑1 (MQ=255)                                                                                                                                        ggCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAg                                                                                                                                                                                                                                     >  2:349814/1‑127 (MQ=255)                                                                                                                                        ggCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAg                                                                                                                                                                                                                                     <  1:349814/127‑1 (MQ=255)                                                                                                                                         gCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCa                                                                                                        <  1:259966/251‑1 (MQ=255)                                                                                                                                           aCCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGATAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATg                                                                                                      >  1:185988/1‑251 (MQ=255)                                                                                                                                                   cgcAGCGATCTTTAATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCAttttt                                                                                              <  1:327105/251‑1 (MQ=255)                                                                                                                                                        cGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGc                                                                                         >  2:75827/1‑251 (MQ=255)                                                                                                                                                        cGATCTTTTATTCGCGATGGGCTGACGCTTCCTCTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGc                                                                                         >  1:169860/1‑251 (MQ=255)                                                                                                                                                                         tGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGCTTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAAc                                                                        >  1:34567/1‑251 (MQ=255)                                                                                                                                                                         tGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAAc                                                                        >  2:262308/1‑251 (MQ=255)                                                                                                                                                                               gACGCTTCCTGTCGATGTGATCGCTAATCCGTAGATTTTCAGGTTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAAGTACCAGCGAAGCTATATCAATATAAATCGCCATGCTTTCGACTTGTGGAGGAAATCGGTTATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTAGCGTGAGGATGATAAATGGCATGGCAGGTTTCGTGCAGACGGCTGGGAGCAACatat                                                                    <  2:150378/249‑1 (MQ=255)                                                                                                                                                                                  gCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccg                                                               >  1:214326/1‑251 (MQ=255)                                                                                                                                                                                    ttCCTTTCGACGTGATAGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGATGTCGCCGCATCCCTAACGCGTAACTACCAGCGAAGCTATCTCACTATTAATCGCCCTGCTTTCGACTTGTGGATGAAATCGCTGCTCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGAGAGGATGATAAATGGCATGACATTTTTCGTGCAGCCGGCTGGGAGCAACATATTAccgcc                                                             >  2:334600/1‑251 (MQ=255)                                                                                                                                                                                     tCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgccc                                                            >  2:70774/1‑251 (MQ=255)                                                                                                                                                                                          gcGATGTGAGCGCGAATCCGCAGATTGTCAGCGTCAAAACCGTCGACGTCGCCGCTTCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTAATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAAGCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTAt                                                       <  1:201803/250‑1 (MQ=255)                                                                                                                                                                                                tgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTc                                                 >  1:272401/1‑251 (MQ=255)                                                                                                                                                                                                  aTCGCTAATCCGCAGATTTTAACCGTCAAAACCGTCGACGTCGCCGCATCGCTACCGCGTAACTCCCAGCGAAGCTATATCAATCTTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCACGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCgg                                               >  2:31989/1‑251 (MQ=255)                                                                                                                                                                                                    cGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGt                                              <  2:51098/250‑1 (MQ=255)                                                                                                                                                                                                     gCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGc                                            >  1:196339/1‑251 (MQ=255)                                                                                                                                                                                                      cTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGcc                                           >  2:315175/1‑251 (MQ=255)                                                                                                                                                                                                            cGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGAGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGgc                                     >  2:223329/1‑251 (MQ=255)                                                                                                                                                                                                                          gTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATggg                       >  1:1355/1‑250 (MQ=255)                                                                                                                                                                                                                             aaaaCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGCAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgcg                    >  1:310471/1‑251 (MQ=255)                                                                                                                                                                                                                              aaaCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAACATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgcgg                   >  2:100980/1‑251 (MQ=255)                                                                                                                                                                                                                               aaCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgcggc                  >  2:322218/1‑251 (MQ=255)                                                                                                                                                                                                                                               gcATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGCCTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGa  <  2:74850/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CGTGGCGATTATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGGCGACATCTCTACCCGGA  >  NC_000913/3869391‑3869879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑