Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,979	G→T	intergenic (‑235/‑302)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,979	0	G	T	100.0%	108.0 / NA	30	intergenic (‑235/‑302)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/16);  total (14/16)

GCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCA  >  NC_000913/3771749‑3772218                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                gCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATcaaaac                                                                                                                                                                                                                             <  1:35281/251‑1 (MQ=255)                  aTCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACactac                                                                                                                                                                                                             <  2:95996/250‑1 (MQ=255)                    cAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTc                                                                                                                                                                                                           <  1:311302/250‑1 (MQ=255)                              tttGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATg                                                                                                                                                                                                >  2:330711/1‑251 (MQ=255)                               ttGGGCTGGAAGCCAGAAGGGAGTCAGGCGGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAAGTGTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAATCCATCAAAACAAAAATGTGACACTACTCACATTTaaa                                                                                                                                                                                                  <  2:153183/248‑1 (MQ=255)                                 gggCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCa                                                                                                                                                                                             <  1:124703/251‑1 (MQ=255)                                                aGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATc                                                                                                                                                                              <  1:330711/251‑1 (MQ=255)                                                                  ttggACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTTCTCTCATTTAAATGCCATTTTTAGCGAAAAGCGCCGCCTTGTTTCtttt                                                                                                                                                             <  2:357416/247‑1 (MQ=255)                                                                    tGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCtttttt                                                                                                                                                           >  1:17923/1‑250 (MQ=255)                                                                        aataatCCGGGTTCGCGATTCACCCCATAACACCAAAGCATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGAATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGCTCACAACTATTAAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTacac                                                                                                                                                       >  2:327462/1‑250 (MQ=255)                                                                              ccGGGTTCGCGATTCTCGCCATAACACCAAAGACTAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGtt                                                                                                                                                >  2:329771/1‑251 (MQ=255)                                                                                       cgATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGCGATGAGTTGCTTAGTTACATAACGATTGTATGAAGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGCAAATATTTAGTGATTCAGATCACAAATATTCAACAAAACATCAAAACAAAAATGTCACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGtgatg                                                                                                                                        >  2:253804/1‑250 (MQ=255)                                                                                                 cATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAacgtcacgtc                                                                                                                             >  1:199467/1‑251 (MQ=255)                                                                                                    aaCACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAAt                                                                                                                          <  1:61632/251‑1 (MQ=255)                                                                                                          aaaGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACctc                                                                                                                    <  1:141542/251‑1 (MQ=255)                                                                                                              aataatTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACctctct                                                                                                                 >  1:165938/1‑250 (MQ=255)                                                                                                                  atTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAGACCATCAAAACAAAAATTTGACACTACTCACATTTAAATGCCAGTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGAGGAACGTCACGGCAATTACCTCTCTAcccc                                                                                                            <  2:244482/251‑1 (MQ=255)                                                                                                                     tttAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTGTTACACAAGCGTTGTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCt                                                                                                          <  2:235816/250‑1 (MQ=255)                                                                                                                                                  aaCGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAgg                                                                            >  1:67056/1‑251 (MQ=255)                                                                                                                                                     gATTGTATGACGAAAGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGCTATCACACAAAAGGCCg                                                                         >  2:55888/1‑251 (MQ=255)                                                                                                                                                           aTGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACt                                                                   >  2:117544/1‑251 (MQ=255)                                                                                                                                                             gACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGAGTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTgg                                                                 <  2:46827/251‑1 (MQ=255)                                                                                                                                                                      aTAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAAc                                                        >  1:244594/1‑251 (MQ=255)                                                                                                                                                                                           cAGGTGAACGCCGAAAGAAAATATCTTGTGATTCAGATCCCAAATATTCAACAAACCATAAAAACAAACATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCACCTTGTTGCTTTTTTACACAAGCGTTTTGAGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATCTCACACAAAAGGCCGTCGAATGGACAGTTAACCGATTCAGTGCCAGATTTCGc                                   >  2:355872/1‑251 (MQ=255)                                                                                                                                                                                                gAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCAATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTAAACAAGCGTTTTGTGATGAACGTCACGTCAATTACATCTCTACCCCCTATATTTATGTGATTGATATAACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTAt                              >  2:31242/1‑251 (MQ=255)                                                                                                                                                                                                         aaGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGt                     <  1:134826/251‑1 (MQ=255)                                                                                                                                                                                                                      gtgATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGGTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTg        <  2:132738/251‑1 (MQ=255)                                                                                                                                                                                                                          ttCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACTAAAGGCCGTCGACTgg                                                                 <  2:45834/190‑1 (MQ=255)                                                                                                                                                                                                                          ttCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACTAAAGGCCGTCGACTgg                                                                 >  1:45834/1‑190 (MQ=255)                                                                                                                                                                                                                            cAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGACAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCca  <  2:244594/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                GCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTAACCGATTCAGTGCCAGATTTCGCAGTATCTACAAGGTCCGGCTACCTCTGCCGCCA  >  NC_000913/3771749‑3772218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑