Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,909	G→A	G151E (GGA→GAA)	bcsE →	c‑di‑GMP‑binding protein BcsE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,909	0	G	A	95.8%	78.0 / NA	24	G151E (GGA→GAA)	bcsE	c‑di‑GMP‑binding protein BcsE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/10);  minor base C (1/0);  total (14/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGGAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAG  >  NC_000913/3696680‑3697146                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              cAAGGGCCGGAAAAAATAAAAATATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCGTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACaa                                                                                                                                                                                                                            <  2:139762/249‑1 (MQ=255)           aaaaaaTAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCAttgttg                                                                                                                                                                                                                <  1:132066/251‑1 (MQ=255)                       tattTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATa                                                                                                                                                                                                     <  1:121475/250‑1 (MQ=255)                          tttCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGt                                                                                                                                                                                                 >  1:285305/1‑251 (MQ=255)                              aaTGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCAc                                                                                                                                                                                             >  2:124431/1‑251 (MQ=255)                               aTGCTAAATCATGAAAAAGGTCTATTCTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTTCCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTGTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCTTTGTTGCTTGAGGAATACCggtctct                                                                                                                                                                                            <  2:344394/251‑7 (MQ=255)                               aTGCTAAATCATGAAAAAGGGCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCAGAGCGGCTTCGCTCATGTTTGGATAAACTGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACt                                                                                                                                                                                            >  1:199792/1‑251 (MQ=255)                                tGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTAGCTTTTGGGAATTAATCCCGAACATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACtt                                                                                                                                                                                           >  2:262634/1‑251 (MQ=255)                                 gCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACttt                                                                                                                                                                                          >  1:137091/1‑251 (MQ=255)                                    aaaTCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTg                                                                                                                                                                                       >  1:223335/1‑251 (MQ=255)                                       tCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTc                                                                                                                                                                                    >  1:7056/1‑251 (MQ=255)                                                                                     agtGATCCCCATAATTACCGTTTTATTCTGGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGAGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGATTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATAtt                                                                                                                                        <  2:128815/247‑1 (MQ=255)                                                                                     aTTGATCCCCATAATTACCTTTTTATTCTTGCTTGAGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGc                                                                                                                                      >  2:331923/1‑251 (MQ=255)                                                                                                          tttATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGCCCAACATTTGCTGGATATTGCCTTCTGGTGCAACGCAAAAgg                                                                                                                 >  1:304694/1‑251 (MQ=255)                                                                                                                cTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTc                                                                                                             >  1:338003/1‑249 (MQ=255)                                                                                                                cTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTc                                                                                                             >  2:112721/1‑249 (MQ=255)                                                                                                                       gcgcAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGt                                                                                                    <  1:145316/251‑1 (MQ=255)                                                                                                                                 cGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTa                                                                                          <  2:7056/251‑1 (MQ=255)                                                                                                                                    aTGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTAATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCg                                                                                       >  1:255401/1‑251 (MQ=255)                                                                                                                                                                                                   gTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCa                        <  1:257684/251‑1 (MQ=255)                                                                                                                                                                                                   gTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCa                        <  1:124431/251‑1 (MQ=255)                                                                                                                                                                                                          cATTGTTCGCTTTTGGTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGcgacg                  >  1:43620/1‑250 (MQ=255)                                                                                                                                                                                                            ttGTTCGCTTTTGGTAATTAATCCCGCAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGaa                >  1:313261/1‑250 (MQ=255)                                                                                                                                                                                                                         gTAATTAATCCCGAAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAg  <  2:121939/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              CAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGATCCCCATAATTACCTTTTTATTCTTGTTTGCGCAAATAACGCATGGCAAAACATTCCTGCCGAGCGGCTTCGCTCATGGTTGGATAAAATGAATAAATGGAGCAGGTTAAACCATTGTTCGCTTTTGGTAATTAATCCCGGAAATAATAACGATAAACAATTTTCATTGTTGCTTGAGGAATACCGTTCACTTTTTGGTCTTGCCAGTTTGCGTTTTCAGGGTGACCAACATTTGCTGGATATTGCCTTCTGGTGCAACGAAAAAGGGGTCAGCGCCCGTCAGCAGCTTAGCGTTCAGCAACAAAATGGTATCTGGACATTAGTTCAAAGCGAAGAGGCGGAGATCCAACCACGCAGCGACGAAAAACGCATTCTGAG  >  NC_000913/3696680‑3697146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑