Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,515	C→T	M311I (ATG→ATA)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,515	0	C	T	100.0%	156.4 / NA	43	M311I (ATG→ATA)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/19);  total (24/19)

GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATAC  >  NC_000913/3682272‑3682759                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTc                                                                                                                                                                                                                                                 >  1:346359/1‑250 (MQ=255)  ccGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGTCGCTATTGTc                                                                                                                                                                                                                                                 >  1:20157/1‑249 (MQ=255)    gTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGt                                                                                                                                                                                                                                             >  2:274228/1‑251 (MQ=255)       aCCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGCCCCGCTCCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAACCCACTACCCGTTCCCCCTGCCGCCCCTTTAGAAAAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGtat                                                                                                                                                                                                                                           <  1:356769/250‑1 (MQ=255)          gcccAGGTTAGTCAGCGCACGACCTTCTGACAAAACGCGGTCGATCCCGAGAATCAGCGCCAGCCCCGCTCCCGGCAAATGGCCCCCCGCAGAGAGCGTCGCCCCCAGCACGATAAAGCCACTACCCTTTACCCCTGCCGCCCCTTTAGAAGAAACCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCg                                                                                                                                                                                                                                       <  1:93219/249‑1 (MQ=255)                gTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGc                                                                                                                                                                                                                                 >  1:191009/1‑251 (MQ=255)                                      gACAAAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTCCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGcc                                                                                                                                                                                                           <  1:7506/251‑1 (MQ=255)                                            aaGCGGTCGATACCGAGGATCAGCGACAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCgg                                                                                                                                                                                                     >  2:345723/1‑251 (MQ=255)                                                       ccggAGGATCAGCGCAAGCCCCGCTCCCGGCAAAGGGCCCACCGCAGAGAGCGTCGCCCCCAGCACAAAAAAGCCACTCCCCGTTCCCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATAAACAGCGTGATTTGGGGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑Gcc                                                                                                                                                                                         <  1:90892/248‑1 (MQ=255)                                                             gATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCccaccc                                                                                                                                                                                   >  2:259253/1‑250 (MQ=255)                                                                      cAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTAc                                                                                                                                                                          >  1:86640/1‑251 (MQ=255)                                                                          cccGCTACCGGAAAAGGCCCCCCCGCAGAGGGCGTCGCCGCCAGCACGATAAAGCCACTCCCCGTTCCCCCTGCCCCCCCTTAAGAAGAAAGCAGCAACACGATTAACAGCGTGATTGGGTGGACGATACCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCa                                                                                                                                                                      <  1:136867/251‑1 (MQ=255)                                                                            cGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGc                                                                                                                                                                    >  1:82710/1‑251 (MQ=255)                                                                                    gCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATTTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGtttt                                                                                                                                                            >  2:251676/1‑250 (MQ=255)                                                                                      aaaTGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTtctc                                                                                                                                                          >  2:279313/1‑251 (MQ=255)                                                                                           gCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCt                                                                                                                                                     >  2:211689/1‑251 (MQ=255)                                                                                                 cGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGTCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGa                                                                                                                                               <  2:20157/251‑1 (MQ=255)                                                                                                                     gCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGCTTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCCGTCGGGATGACCA‑GCCCCACCACCGATTTACCGCAGCCGAGTTTCTCCAGCTTGGCGAACATACGCGGCAGCGCAGAc                                                                                                                           >  2:267095/1‑251 (MQ=255)                                                                                                                                  aCTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGCAGATGa                                                                                                                >  1:319550/1‑249 (MQ=255)                                                                                                                                          ttcccccGGCCGCCCCTTTAAAAGAAAGCAGCAACACGATAAACGGCGTGTTTTGGTGGACGATATCCATCTGAGTGTTG‑GTGGCCGGGGCGATAAC‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAg                                                                                                       <  2:327716/247‑1 (MQ=255)                                                                                                                                              cccTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGTCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACa                                                                                                   >  2:246677/1‑250 (MQ=255)                                                                                                                                                 tGCCGCCCATTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTCCAAta                                                                                                >  2:359193/1‑249 (MQ=255)                                                                                                                                                           ttAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                                     >  1:267502/1‑251 (MQ=255)                                                                                                                                                            tAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                                     >  2:88185/1‑250 (MQ=255)                                                                                                                                                            aagaaaaAAGCAGCAACCCGATAAACAGCTTGATTTGGTGGACGATATCCATCGGACTGTTA‑GGGGCCTGGGCGAAAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGttcttc                                                                                     <  1:185879/249‑1 (MQ=255)                                                                                                                                                             agaagaAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCAc                                                                                   >  2:18904/1‑251 (MQ=255)                                                                                                                                                                              cattaaactgcgttattgtgtggACGATACCCATTTGACTTTTA‑GGGGCGGGGGCGAAAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCATTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAt                                                                  <  2:202594/232‑1 (MQ=255)                                                                                                                                                                              cGATGAACAGCGTGATTGGGTTGACGATACCCACCTGACTGTTA‑GTGGCCGGGGCAATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAt                                                                  <  1:348516/251‑1 (MQ=255)                                                                                                                                                                               gATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAtt                                                                 <  1:211689/251‑1 (MQ=255)                                                                                                                                                                               aaTTAACAGGGTGATTGGGTGGCCGATACCCATCTGATTTTAA‑GTGGCCGGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAAtt                                                                 <  2:205779/250‑1 (MQ=255)                                                                                                                                                                                  taacgtggtattgtggtgaACGATATCCATCTG‑CTGTTAAGTGGC‑TGGGGGAAAAAACACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTg                                                               <  2:122383/237‑1 (MQ=255)                                                                                                                                                                                          tGATTTGGCGGACGATATCCAACTGACTGTTA‑GTGGACTGCGCGATAAC‑CACCGCAGATATTGTCAGGTATATCGATGTGCCATCAAGGATAAACGAGTCGACTGTCGGGATGACCA‑GCCCCCCCACCGATTTACAGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGAAAAATTTGAAGCTACTg                                                      >  2:25429/1‑251 (MQ=255)                                                                                                                                                                                             tttGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGaa                                                    >  1:192733/1‑250 (MQ=255)                                                                                                                                                                                                       gATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCtt                                         >  2:61121/1‑251 (MQ=255)                                                                                                                                                                                                           cccATCTGAATTTTA‑GTGGCTGGGGCGAAAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGc                                     <  1:314208/250‑1 (MQ=255)                                                                                                                                                                                                                cTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCAGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGCCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTg                                >  1:203135/1‑251 (MQ=255)                                                                                                                                                                                                                  gACTGTTA‑GTGGCCGGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGAtt                                 <  1:18904/248‑1 (MQ=255)                                                                                                                                                                                                                    cTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGCAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAAcc                            >  2:201647/1‑251 (MQ=255)                                                                                                                                                                                                                       gtttgTGGCCTGGGCGTTAAA‑CACCGCCGCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGaa                              <  1:328950/243‑1 (MQ=255)                                                                                                                                                                                                                                   ggCGATAAA‑CACCGCCGCTCTTTTCAGGTATATCGCTGTGCCATCAAGGTTCAACGCGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGCGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGCCTCGGAAGATGAAGCCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGCTAAATTTGAAGATACTTAAACCAGTCTCTTTCGCGATTGAACCACATACCCGcaccc              >  2:305578/1‑250 (MQ=255)                                                                                                                                                                                                                                       aataacccccgccgCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACaa           <  1:246677/243‑1 (MQ=255)                                                                                                                                                                                                                                              cccgccgCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGAt    <  2:207284/250‑1 (MQ=255)                                                                                                                                                                                                                                               ccgccgCTATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATa   <  2:82710/251‑1 (MQ=255)                                                                                                                                                                                                                                                  ccgctATTGACAGGTATATAACTGTGCCATCAAGGATCAACGAATAGCAAGTCGGCTTG‑CCACGCCCCACCACACATTTACGGCAGCCGCGTTTCTCCATCTTGTCGCGCATACGCGGCCGCGCCGACTCGGGAGATGAAGCCGCCCGTACTGTCAGCAGGTCTTCCCGCAAGAACCGGATAAATTTGACGATACGGAAACCAGTCGCTTTAGCGATTGAACCCACCACCACCATCACAAACAGGATAc  >  2:322076/1‑249 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTA‑GTGGCCTGGGCGATAAA‑CACCGCCGCCATTGTCAGGTATATCGATGTGCCATCAAGGTTAAACGAGTAGCCTGTCGGGATGACCA‑GCCCCACCACCGATTTACGGCAGCCGAGTTTCTCCATCTTGTCGAGCATACGCGGCAGCGCCGACTCGGAAGATGAAGTCCCCAGTACAATCAGCAGTTCTTCACGGATGTAGCGGATAAATTTGAAGATACTGAAACCAGTCGCTTTAGCGATTGAACCCAATACCAGCACCACAAACAGGATAC  >  NC_000913/3682272‑3682759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑