Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,543,877	G→A	P237P (CCG→CCA)	rpnA →	recombination‑promoting nuclease RpnA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,543,877	0	G	A	100.0%	97.7 / NA	27	P237P (CCG→CCA)	rpnA	recombination‑promoting nuclease RpnA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/16);  total (11/16)

GCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCGAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCT  >  NC_000913/3543631‑3544087                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                   gCTGATTGATGTCACCGTCATGCCTGATGATGAAAGCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATGTTATCGACGGCGTTGCCGAACGTTCACCAAAac                                                                                                                                                                                                                <  2:50381/251‑1 (MQ=255)                 gTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAAt                                                                                                                                                                                                <  1:68094/251‑1 (MQ=255)                       ccTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTAt                                                                                                                                                                                          <  1:113326/251‑1 (MQ=255)                       ccTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTAt                                                                                                                                                                                          >  2:301706/1‑251 (MQ=255)                                     cATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGc                                                                                                                                                                            <  1:51071/251‑1 (MQ=255)                                                   gTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGaa                                                                                                                                                              <  2:67821/251‑1 (MQ=255)                                                     aTGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTATTTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGGAGGAGGGGGAAc                                                                                                                                                             >  2:243813/1‑250 (MQ=255)                                                                       aTTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATGTTATCGACGGCGTTGCCTAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGAGTGCGGCATGAGGGTGAACAATCCTAAGCCCTGCatat                                                                                                                                          <  2:144138/251‑1 (MQ=255)                                                                           aaaaaCATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGcc                                                                                                                                      <  1:304300/251‑1 (MQ=255)                                                                                         cAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGTGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTTCGGCAGGAGGTGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCttt                                                                                                                         <  1:122474/250‑2 (MQ=255)                                                                                             gcgACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATcc                                                                                                                    <  1:243813/251‑1 (MQ=255)                                                                                               gACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATTCTTGAATCCgg                                                                                                                  >  2:87832/1‑251 (MQ=255)                                                                                                 ccttaTGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAg                                                                                                                <  2:3540/247‑1 (MQ=255)                                                                                                             ggTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCa                                                                                                     <  1:274574/250‑1 (MQ=255)                                                                                                                   gCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGAcatca                                                                                              <  1:37201/251‑1 (MQ=255)                                                                                                                     aaaTGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATg                                                                                            >  1:125054/1‑251 (MQ=255)                                                                                                                         ggCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATgcgc                                                                                         <  2:97279/250‑1 (MQ=255)                                                                                                                                  attaAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGCTTGCGGCAGGAGGGGGAACAATCCAAAGCCCCGCATATAGCCAAAATAATGCTTGAATCCGGAGTTACTCTTGCAGACATCATGCGCtttcacgggc                                                                               >  2:239422/1‑244 (MQ=255)                                                                                                                                     aaGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGt                                                                            >  2:304749/1‑251 (MQ=255)                                                                                                                                     aaGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGt                                                                            <  2:115855/251‑1 (MQ=255)                                                                                                                                          gtGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGTGAACAAGCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCTGAGTTGCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCagaa                                                                       <  1:34185/251‑1 (MQ=255)                                                                                                                                             gATACGCTAATGACAGACAAATCAAAGGTCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGATAGTTTAATGACTAtt                                                                                                                                                                                         <  1:286214/134‑1 (MQ=255)                                                                                                                                             gATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTAtt                                                                                                                                                                                         >  2:286214/1‑134 (MQ=255)                                                                                                                                                                                    aCATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGcc                              >  2:127852/1‑250 (MQ=255)                                                                                                                                                                                     cATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCAt                            >  1:222266/1‑251 (MQ=255)                                                                                                                                                                                         cTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGGCAGTAAAGTTCTGTCTCGCCATTTc                         >  1:148293/1‑250 (MQ=255)                                                                                                                                                                                                               gTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCAAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTGGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGCAGAAGAGTTGGCTGCGGCGAGCCAGTAAAGGTCTGCCTCGCCATTTCAAAAGTCACCGACACCGTCTGCt  >  2:12013/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                   GCTGATTGATGTCACCGTCATGCCTGATGATGAAATCATGCAGCACCGACGTATGGCGCTGCTGGAGTTAATTCAAAAACATATTCGTCAACGCGACCTGATGGGGCTGGTAGAGCAAATGGCCTGCTTATTAAGTAGTGGATACGCTAATGACAGACAAATCAAAGGGCTGTTTAATTACATACTGCAAACTGGCGACGCTGTACGTTTTAACGATTTTATCGACGGCGTTGCCGAACGTTCACCGAAACACAAGGAGAGTTTAATGACTATTGCGGAAAGATTGCGGCAGGAGGGGGAACAATCCAAAGCCCTGCATATAGCCAAAATAATGCTTGAATCCGGAGTTCCTCTTGCAGACATCATGCGCTTTACCGGGCTGTCAGAAGAAGAGTTGGCTGCGGCGAGTCAGTAAAGTTCTGTCTCGCCATTTCAAAAGCCACCTACACCCTCTGCT  >  NC_000913/3543631‑3544087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑