Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,768	C→T	W362* (TGG→TGA)	chiA ←	endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,768	0	C	T	100.0%	105.2 / NA	32	W362* (TGG→TGA)	chiA	endochitinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

TGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTG  >  NC_000913/3468528‑3469006                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               tGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGACCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCt                                                                                                                                                                                                                                      >  2:177305/1‑251 (MQ=255)   cGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTg                                                                                                                                                                                                                                    >  1:135759/1‑251 (MQ=255)    gTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                   >  1:324505/1‑251 (MQ=255)    gTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGGTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGt                                                                                                                                                                                                                                   <  1:240511/251‑1 (MQ=255)            cGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACAtt                                                                                                                                                                                                                            >  1:256118/1‑250 (MQ=255)               cAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTAcc                                                                                                                                                                                                                         >  2:133286/1‑250 (MQ=255)                   aGTTTTATAATGTGAATCGTTATAATCTCCGTTGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGa                                                                                                                                                                                                                    <  2:77401/251‑1 (MQ=255)                      gttacaattggttacGTTATAATTGACTATGCTGTACGCGTTGTACCCTTTGCATGCCTGGAAACCATCCGAAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGTCTCAATTCCTCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGTAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGAACCAACCCATCAGGCTTTCTTGTAAACATTACCATCGaaa                                                                                                                                                                                                                  <  2:348828/236‑1 (MQ=255)                                     gTTATAATCTACGATGCTGTCCGCACTGTACGCTTTGTTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAAttt                                                                                                                                                                                                  <  1:141570/251‑1 (MQ=255)                                               aCGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTAcc                                                                                                                                                                                           <  1:271433/248‑1 (MQ=255)                                                 gATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCCATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAACATAACATGGGAACTTTCGCTACCTTCCt                                                                                                                                                                                      >  2:229679/1‑251 (MQ=255)                                                                         gCTGGCCTGGAACCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTGGCAATGAg                                                                                                                                                               <  2:347055/250‑1 (MQ=255)                                                                           gggCCTGGAAACCACCCACAACAAGGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGGGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTaa                                                                                                                                                            <  2:124422/250‑1 (MQ=255)                                                                                   aaaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTTCCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAAc                                                                                                                                                    <  1:211285/251‑1 (MQ=255)                                                                                   aaaCCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAAc                                                                                                                                                    <  1:14959/251‑1 (MQ=255)                                                                                                       gccgccGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGAGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATg                                                                                                                                >  1:230674/1‑251 (MQ=255)                                                                                                                              tAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATCGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTggg                                                                                                         >  1:76538/1‑251 (MQ=255)                                                                                                                                  cGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTc                                                                                                      >  1:91034/1‑250 (MQ=255)                                                                                                                                    ggTTACCGTACTGACTCAATTCCGCAGCGTTAGCTGTGCGCTCGAGACGCCATGGGGTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTTGCTACCGGCCTGGCTGCTTCACGCTTGCAATGAGTAATCGGTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCAcc                                                                                                   <  2:143657/251‑1 (MQ=255)                                                                                                                                     gTTACCGTACTGACTCAATTCCGCAGCGGGAGCTGGACGCTCGAGACGCCATGGGTTATTGGAGTGTTCAGCGCTGGTACCCCGTGGGCAATCGTTAGACCCAGCCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTGCCTGGTCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGGTGATGGCTCACTGTTATCCTGAGGTGGGCTCACCg                                                                                                  <  2:49951/251‑1 (MQ=255)                                                                                                                                            tACTGACTCCATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTc                                                                                           >  1:97031/1‑251 (MQ=255)                                                                                                                                             aCTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTcc                                                                                          <  1:99411/251‑1 (MQ=255)                                                                                                                                                    cAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTg                                                                                   <  2:78268/251‑1 (MQ=255)                                                                                                                                                                 gTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGt                                                                      >  1:125938/1‑251 (MQ=255)                                                                                                                                                                       gTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGc                                                                <  1:238099/251‑1 (MQ=255)                                                                                                                                                                       gTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGc                                                                <  1:173145/251‑1 (MQ=255)                                                                                                                                                                        gaCGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTTCCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCa                                                               <  2:318857/250‑1 (MQ=255)                                                                                                                                                                           gcgcGAGACGCCATGGGTTAGTGGAGTGTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATACGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTGTCCGGAGTTGGATCAACCGGAGTCACAGCACCg                                                            <  2:141420/248‑1 (MQ=255)                                                                                                                                                                                                                    ccccGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAcc                   >  1:42613/1‑251 (MQ=255)                                                                                                                                                                                                                     cccGTGGGCAATCTTTAGACCCAACCCATCAGGCGTTCTTGTACACCTTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTAccc                  >  1:30252/1‑250 (MQ=255)                                                                                                                                                                                                                                 cTTTAGACCCAACCCATCACGCGCTCTTGTAAACATTACCATTGAAAATACCATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGATTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGCAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTGTTATCCGGCTTAACGGAACAGGAGCCCGGGTTACCATACTGGCTgctt      >  2:225760/1‑248 (MQ=255)                                                                                                                                                                                                                                     aGACCCAACCCATCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCtt  >  2:181417/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               TGCGTCTTGATCGACAGAAGTTTTATAATGTGCATCGTTATAATCTACGATGCTGTCCGCGCTGTACGCTTTGCTGGCCTGGAAACCATCCGCAACAATGACGCCGCCGTTATCAATTTCACAGGTAGTCGGGTTACCGTACTGACTCAATTCCGCAGCGGTAGCTGTACGCTCGAGACGCCATGGGTTATTGGAGTTTTCAGCGCTGGTACCCCGTGGGCAATCTTTAGACCCAACCCACCAGGCGTTCTTGTAAACATTACCATTGAAAATAACATGGTAAATTTCGCTACCTTCCTGGCCGCTCCACGCTTGCAATGAGTAATCGTTAACGCTATCCGCTGGTGTTGATGGCTCGCTGTTATCCGGAGTTGGGGTCACCGGTGTTTCCGGAGTTGGATCAACCGGAGTCACAGCACCGCCATTATTATCCGGCTTAACGGAACAGGAGCCAGGGTTACCATACTGGCTGATTTCTG  >  NC_000913/3468528‑3469006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑