Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,449,779 G→A S35S (AGC→AGT)  rpsC ← 30S ribosomal subunit protein S3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,449,7790GA97.0% 107.6 / NA 34S35S (AGC→AGT) rpsC30S ribosomal subunit protein S3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (13/19);  minor base C (0/1);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCG  >  NC_000913/3449546‑3450019
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
gCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGc                                                                                                                                                                                                                                 <  2:206360/251‑1 (MQ=255)
 cGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCg                                                                                                                                                                                                                                >  1:8751/1‑251 (MQ=255)
      aGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGCGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGACTTc                                                                                                                                                                                                                           >  2:253077/1‑251 (MQ=255)
            ggCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCGCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGGCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgt                                                                                                                                                                                                                     <  1:128870/251‑1 (MQ=255)
              cTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCAACTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGgtgt                                                                                                                                                                                                                   >  2:333333/1‑251 (MQ=255)
                      cTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAAc                                                                                                                                                                                                           >  1:304639/1‑251 (MQ=255)
                         cGGCGATGTTGAGCTGTGCAGGAACGCCTGCGATGTCCGCTACGTCCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATTCCCGGGCGAGCAGTGTTAATTGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAg                                                                                                                                                                                                        <  1:348133/251‑1 (MQ=255)
                                   gATCTGTGCAGGAACGCCAGCGATGTACGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACTCGGATGCTCGTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTc                                                                                                                                                                                               <  2:118005/250‑1 (MQ=255)
                                           cAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGtttt                                                                                                                                                                                      <  2:129294/251‑1 (MQ=255)
                                             ggAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGACAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTAc                                                                                                                                                                                    >  2:248802/1‑251 (MQ=255)
                                                      gCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAg                                                                                                                                                                           <  1:270462/251‑1 (MQ=255)
                                                                  aCGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCTGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATTCGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCATGCGAATACCa                                                                                                                                                                 <  2:229902/249‑1 (MQ=255)
                                                                        ttACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATg                                                                                                                                                         >  2:64632/1‑251 (MQ=255)
                                                                                   ttCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATTCCATTATGATGTACTTTCTGa                                                                                                                                               <  1:114017/250‑1 (MQ=255)
                                                                                       aCGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCAt                                                                                                                                          >  1:154005/1‑251 (MQ=255)
                                                                                        cGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCAtt                                                                                                                                         <  2:42502/251‑1 (MQ=255)
                                                                                                                            gAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATTCCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGGCTCAGCGATCGGACACAACca                                                                                                     <  2:100698/251‑1 (MQ=255)
                                                                                                                                      aaTGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGAtgtg                                                                                           <  2:312312/251‑1 (MQ=255)
                                                                                                                                                 ggATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGGTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACGTTCTGGCCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGTCGCAACCACAGTGATGTGTCTGGTGCGCt                                                                                <  2:149570/251‑1 (MQ=255)
                                                                                                                                                  gATGCTCTTCGCGGGACGCTCGATAACGATAAGAGATACGCACGCTTTAGCCAGTTCCTTCGTCAGCTAATGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCACACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGATGGTggggtt                                                                               >  2:29135/1‑246 (MQ=255)
                                                                                                                                                  gATGCTCTTAACCGAACGCTCGATAACGATCCGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGGGCtt                                                                               >  2:233565/1‑251 (MQ=255)
                                                                                                                                                           aGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCGTCAGGATGCg                                                                      >  2:287636/1‑251 (MQ=255)
                                                                                                                                                                 aCGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGc                                                                <  1:64632/251‑1 (MQ=255)
                                                                                                                                                                            cGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTg                                                     <  2:318844/251‑1 (MQ=255)
                                                                                                                                                                                aCGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGGGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcg                                                 >  1:80974/1‑251 (MQ=255)
                                                                                                                                                                                 cGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCAcgc                                                >  1:230180/1‑251 (MQ=255)
                                                                                                                                                                                                 tAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTc                                 <  2:87960/250‑1 (MQ=255)
                                                                                                                                                                                                             tAGTCAGGTACTGACGTACTTTAAAATCTCTGTCCAGTTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGATTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCGTCAGGAGGCGATCTGCACGTCCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGcc                      <  1:217448/249‑1 (MQ=255)
                                                                                                                                                                                                             tAGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTTGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGcc                      <  1:253077/249‑1 (MQ=255)
                                                                                                                                                                                                              aGTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTGTTACAATACCCAGGCGAAGACCATTAGGATGTACTTTCTGACCCAGTGCTAGTCTCCAGAGTCTCAGCGATCGGACACATCCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCGGCACGTCCTTTGGCACGCGGCATAATGCGCTTCATGCTCGGGCCtt                    <  2:335396/250‑1 (MQ=255)
                                                                                                                                                                                                               gTCAGGTACTGACGTACTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTGCAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCg                  <  1:10915/251‑1 (MQ=255)
                                                                                                                                                                                                                           cGTACTTTAAAATCCCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAAGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATCCCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCGGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCTCGCTGCATAATGCTCTTCATGCTCGGGCCTTCGTCTACAAAAAtt      <  2:296922/251‑1 (MQ=255)
                                                                                                                                                                                                                              aCTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAAtttt    <  1:123124/250‑1 (MQ=255)
                                                                                                                                                                                                                               cTTTAAAATCACTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGGTCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCCTGCTCGGGCCTTCGTCTACGAAAATTTTCg  >  2:269628/1‑251 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
GCGTCCAGTTCAGGCTTACGAACTTCGGCGATGTTGATCTGTGCAGGAACGCCAGCGATGTCCGCTACGACCTTACGCAGTTTTTCTACGTCTTCACCTTTTTTACCGATAACGATACCCGGGCGAGCAGTGTGAATGGTTACACGGATGCTCTTAGCCGGACGCTCGATAACGATACGAGATACGGACGCTTTAGCCAGTTCCTTAGTCAGGTACTGACGTACTTTAAAATCGCTGTCCAGGTTGTCAGCGAATTCTTTGGTGTTCGCAAACCAGGTAGAGTTCCATGGTTTTACAATACCCAGGCGAATACCATTAGGATGTACTTTCTGACCCATTGCTAGTCTCCAGAGTCTCAGCGATCGGACACAACCACAGTGATGTGGCTGGTGCGCTTCAGGATGCGATCTGCACGACCTTTTGCACGCGGCATAATGCGCTTCATGCTCGGGCCTTCGTCTACGAAAATTTTCG  >  NC_000913/3449546‑3450019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑