Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,436,697	G→A	E60E (GAG→GAA)	trkA →	NAD‑binding component of Trk potassium transporters

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,436,697	0	G	A	96.9%	104.7 / ‑7.0	33	E60E (GAG→GAA)	trkA	NAD‑binding component of Trk potassium transporters
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (14/17);  total (15/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCC  >  NC_000913/3436456‑3436937                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 agGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGcc                                                                                                                                                                                                                                         >  2:32018/1‑251 (MQ=255)         ggCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGc                                                                                                                                                                                                                                 >  1:115616/1‑251 (MQ=255)                       taagctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTg                                                                                                                                                                                                                   >  1:289846/1‑251 (MQ=255)                          gctaaTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATGCCAACGGGGAGCGTCTGCGGACCTTACAGGATAAATTTGTCCTGCGGGGCGTGCAGGGGTGTGGCTCGCATCCACGGTTGTGGCGGGAGGCATGTGCCGACGACGGCGATATGCTGGTTGCTg                                                                                                                                                                                                                <  1:331784/251‑1 (MQ=255)                             aagcAAAAAGTGATGAGATAACGGGTCGCGGCTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTGGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATGTGACCTGTGGGCCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCTGGAAGCAGGTGCCGCCGACGCCGATATGCTGGTTGCTGTa                                                                                                                                                                                                              <  2:180170/247‑1 (MQ=255)                              aTCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGGGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTAATGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAAc                                                                                                                                                                                                            >  2:162724/1‑251 (MQ=255)                                  aaaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGt                                                                                                                                                                                                        <  1:83548/251‑1 (MQ=255)                                   aaaGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGtt                                                                                                                                                                                                       <  1:114171/251‑1 (MQ=255)                                      gTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAg                                                                                                                                                                                                    <  1:335604/251‑1 (MQ=255)                                        gatgaGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGAt                                                                                                                                                                                                  <  1:336328/251‑1 (MQ=255)                                                              gatgaAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCGGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTtgcctg                                                                                                                                                                              <  1:43603/249‑1 (MQ=255)                                                                                    gCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCa                                                                                                                                                       <  1:231569/250‑1 (MQ=255)                                                                                     ccGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAac                                                                                                                                                     >  1:135263/1‑251 (MQ=255)                                                                                       ggCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGGCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGGTGAAACCAATATGGTTGCCTGCCAGGGAGCCTACTCACTTTTCAacac                                                                                                                                                   >  2:261560/1‑251 (MQ=255)                                                                                                                aaaaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGc                                                                                                                          >  2:346441/1‑251 (MQ=255)                                                                                                                 aaaCCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCt                                                                                                                         <  2:321976/251‑1 (MQ=255)                                                                                                                       ggTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCACTCAcc                                                                                                                     <  2:117587/249‑1 (MQ=255)                                                                                                                                    aacGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGCCTACGt                                                                                                            >  2:345371/1‑245 (MQ=255)                                                                                                                                    aacGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGt                                                                                                            <  1:345371/245‑1 (MQ=255)                                                                                                                                        atatTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTGGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCGAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGcc                                                                                                  <  1:344273/251‑1 (MQ=255)                                                                                                                                            tACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGAt                                                                                               <  2:47293/250‑1 (MQ=255)                                                                                                                                                     cGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCTCCAGACTACGTGCGCGATGCCGATAAGCTATttc                                                                                     <  1:52460/251‑1 (MQ=255)                                                                                                                                                       aTACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATttcat                                                                                   >  1:132008/1‑251 (MQ=255)                                                                                                                                                                gtcaGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCt                                                                          >  2:279738/4‑251 (MQ=255)                                                                                                                                                                gTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCt                                                                          >  2:279744/1‑251 (MQ=255)                                                                                                                                                                        ctgaggaaggtaaaggctaactttGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGAt                                                                  >  1:133011/22‑251 (MQ=255)                                                                                                                                                                                        aTAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATc                                                                                                                             <  1:112534/176‑1 (MQ=255)                                                                                                                                                                                        aTAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATc                                                                                                                             >  2:112534/1‑176 (MQ=255)                                                                                                                                                                                              ttGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCaga                                             <  2:282930/250‑1 (MQ=255)                                                                                                                                                                                                                aGGGGCATGGCTCTCCTCCACGCGTATTGCGGGATGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGataat                          >  1:255106/1‑251 (MQ=255)                                                                                                                                                                                                                aGGGGCATGGCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGataat                          >  2:209469/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCTCTCATCCACGCGTATTGCGGGAAGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTAcc                   <  2:109892/250‑1 (MQ=255)                                                                                                                                                                                                                                         aTTGCGGGAAGAAGGTGCCGCCGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCTCTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATccc  <  2:167081/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 AGGGCGACGGCTTCTTTTACGCTAAGCTAATCAAAAAGTGATGAGATAACGGGTCGCGACTGATGAAAATTATCATTCTGGGTGCCGGCCAGGTTGGCGGCACACTGGCGGAAAACCTGGTTGGCGAGAACAACGATATTACTGTTGTCGATACCAACGGTGAGCGTCTGCGGACCTTACAGGATAAATTTGACCTGCGGGTCGTGCAGGGGCATGGCTCTCATCCACGCGTATTGCGGGAGGCAGGTGCCGACGACGCCGATATGCTGGTTGCTGTAACCAGTTCAGATGAAACCAATATGGTTGCCTGCCAGGTAGCCTACTCACTTTTCAACACCCCTAATCGCATCGCTCGTATCCGCTCACCAGACTACGTGCGCGATGCCGATAAGCTATTTCATTCAGATGCTGTGCCGATTGATCATCTGATCGCACCAGAGCAGTTGGTTATCGATAATATTTACCGACTGATTGAGTATCCC  >  NC_000913/3436456‑3436937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑