Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,292	C→T	intergenic (+96/‑16)	queF → / → ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,292	0	C	T	100.0%	79.2 / NA	23	intergenic (+96/‑16)	queF/ppnN	7‑cyano‑7‑deazaguanine reductase/nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/11);  total (12/11)

GCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTG  >  NC_000913/2926051‑2926535                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    gCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAgg                                                                                                                                                                                                                                              >  1:274706/1‑249 (MQ=255)       ttAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGttttt                                                                                                                                                                                                                                       <  2:48794/250‑1 (MQ=255)                 gTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTAGTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTac                                                                                                                                                                                                                              <  1:17640/249‑1 (MQ=255)                          cTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGcc                                                                                                                                                                                                                   >  1:269850/1‑251 (MQ=255)                                            gAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGata                                                                                                                                                                                                 <  1:318433/251‑1 (MQ=255)                                                         cGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCt                                                                                                                                                                                    <  1:279754/251‑1 (MQ=255)                                                                     cgtGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGatat                                                                                                                                                                        <  2:98306/251‑1 (MQ=255)                                                                                                                       aTCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGtt                                                                                                                      >  1:137682/1‑251 (MQ=255)                                                                                                                        tCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCa                                                                                                                                       <  2:128708/233‑1 (MQ=255)                                                                                                                        tCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCa                                                                                                                                       >  1:128708/1‑233 (MQ=255)                                                                                                                                       gTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGa                                                                                                       >  2:286768/1‑250 (MQ=255)                                                                                                                                            gCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCg                                                                                                 <  1:323809/251‑1 (MQ=255)                                                                                                                                                  aaTTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGCACGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGtt                                                                                           >  1:47937/1‑251 (MQ=255)                                                                                                                                                                                 cGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCACAGAATTGCTGTCTCg                                                            >  2:308896/1‑251 (MQ=255)                                                                                                                                                                                  gCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGGGGATATGCTTAAACGCCCCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGt                                                           <  2:269850/251‑1 (MQ=255)                                                                                                                                                                                           tgtgAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGaaaa                                                   <  1:171060/250‑1 (MQ=255)                                                                                                                                                                                                   gTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGatat                                          >  2:40762/1‑251 (MQ=255)                                                                                                                                                                                                                    ttGTAATCAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCTGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTg                         >  2:251553/1‑251 (MQ=255)                                                                                                                                                                                                                           cAAAGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAAcgcg                   <  1:40762/250‑1 (MQ=255)                                                                                                                                                                                                                            aaaGGGAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACgcggc                 <  2:66521/251‑1 (MQ=255)                                                                                                                                                                                                                               gggAATGACGATATTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGt               >  1:252365/1‑250 (MQ=255)                                                                                                                                                                                                                                    tGACGATATTCGTCTCCTAAGGAGTTTTTCTTGATTACACTTATTCGCCCGCTTGGCTCAATGGATATGTTGTCGAACATGGACGTGGATATGCTTAAACGCACCGCCAGCAGCGACATCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTAAACTCCGGTAGTTTGACCGTAAACAGCAAAGAATTGCTGCTTCGTTTTGAAAATTCCGATATAATCGTCTGGGGCCGTGACCGCGGCGTAAAGcc         >  2:288138/1‑250 (MQ=255)                                                                                                                                                                                                                                           atTCGTCTCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTg  >  2:212861/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                    GCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGCAATAAATTTTTTCTCAATTTTGCGTGCTGGATTCACGCAGAAGGTTGTGAAAGGTCATCAGGCAGGGCTATTGTAATCAAAGGGAATGACGATATTCGTCCCATAAGGAGTTTTTCTTGATTACACATATTAGCCCGCTTGGCTCCATGGATATGTTGTCGCAGCTGGAAGTGGATATGCTTAAACGCACCGCCAGCAGCGACCTCTATCAACTGTTTCGCAACTGTTCACTTGCCGTACTGAACTCCGGTAGTTTGACCGATAACAGCAAAGAATTGCTGTCTCGTTTTGAAAATTTCGATATTAACGTCTTGCGCCGTGAACGCGGCGTAAAGCTGGAACTG  >  NC_000913/2926051‑2926535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑