Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,825	C→T	Q39* (CAA→TAA)	ygcG →	TPM domain‑containing protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,825	0	C	T	100.0%	61.1 / NA	18	Q39* (CAA→TAA)	ygcG	TPM domain‑containing protein YgcG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

ATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAACC  >  NC_000913/2905583‑2906064                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                aTTTAATCATGTCAAACCATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATCACGAATATAAAATTACTGCTCCCCCCGTCACTTTATTTATCCCGATTTAATGAGCAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACTCACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATaagaaaa                                                                                                                                                                                                                                          >  2:305581/1‑250 (MQ=255)                 aCATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGcac                                                                                                                                                                                                                          >  1:303336/1‑250 (MQ=255)                                     tatttTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCTATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGGGACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCaa                                                                                                                                                                                                      <  2:67709/250‑1 (MQ=255)                                                 aTTAGTTTTTAATGATAACGAATATAAAATTAACGATGCCGCCGTCCATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTg                                                                                                                                                                                         >  1:221179/1‑251 (MQ=255)                                                  ttAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTGTAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGc                                                                                                                                                                                        >  2:172668/1‑251 (MQ=255)                                                                                          ccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTTTTGCAGCCCAACCAACTATTGTCCCACAATTCCAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGCCGAGAc                                                                                                                                                <  1:242460/251‑1 (MQ=255)                                                                                          ccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATGTTCAATAATACCCAAGTGCAAAGGGGCTTATTAATTGTGCCCACAACCAAAGACgagtc                                                                                                                                                <  2:23268/251‑3 (MQ=255)                                                                                                               tttAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACa                                                                                                                             >  1:241134/1‑249 (MQ=255)                                                                                                                  aaTGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAagag                                                                                                                         <  1:144317/250‑1 (MQ=255)                                                                                                                                        aTTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAg                                                                                                                                                                                                                   >  1:67892/1‑138 (MQ=255)                                                                                                                                        aTTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAg                                                                                                                                                                                                                   <  2:67892/138‑1 (MQ=255)                                                                                                                                                                 aTGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATAc                                                                         >  2:281572/1‑251 (MQ=255)                                                                                                                                                                           gttgCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCgttgt                                                               <  2:287569/251‑1 (MQ=255)                                                                                                                                                                                           cTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGc                                               >  2:310582/1‑251 (MQ=255)                                                                                                                                                                                                      caATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATGCTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCAt                                    <  1:225635/251‑1 (MQ=255)                                                                                                                                                                                                            cAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCaggt                              <  1:121835/251‑1 (MQ=255)                                                                                                                                                                                                                                tACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGgaaga            <  1:172668/249‑1 (MQ=255)                                                                                                                                                                                                                                        gCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAAcc  <  2:48442/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                ATTTAATCATGTCAAAACATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAACC  >  NC_000913/2905583‑2906064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑