Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,077	C→T	intergenic (+459/+492)	iap → / ← cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,077	0	C	T	100.0%	72.8 / NA	22	intergenic (+459/+492)	iap/cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

CTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCCCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGG  >  NC_000913/2877836‑2878308                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        cTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGgcgc                                                                                                                                                                                                                                >  1:145805/1‑251 (MQ=255)        ggggAACGCGTGGGCCATCCTTCCACCTATGTCTGAACTGCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAAc                                                                                                                                                                                                                         <  2:101511/251‑1 (MQ=255)                        atcatTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTAtcc                                                                                                                                                                                                         >  2:193354/1‑250 (MQ=255)                                                                      gggAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACt                                                                                                                                                           >  2:211042/1‑251 (MQ=255)                                                                           cTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCCAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGTGTTATCCCCGCTGGCGCGGGGAACTCGc                                                                                                                                                        >  1:228333/1‑249 (MQ=255)                                                                                   ggATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGAc                                                                                                                                              <  1:84055/251‑1 (MQ=255)                                                                                                 gTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATgcg                                                                                                                                >  1:26399/1‑251 (MQ=255)                                                                                                      gcgcCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGAtt                                                                                                                           <  1:193354/251‑1 (MQ=255)                                                                                                                             ggCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCTCCGCTGgcg                                                                                                      <  1:85740/249‑1 (MQ=255)                                                                                                                                gaggggAACTCTGGGCGGCTTGCCTTGCAGCCAGATCCAGCAGCGGTTTATCACCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGATTATCCTCGCTGGCGCGGGGCACTCTAGTTTCCGTATCTCCGGATTTATAACGCTGACGGTTTATCCCAGCTGGCGCGGGGCGATCGCAGGCGGCGACGAGCAGGGTATGTGCGTTTCGCGGGTTATcccgcatcgcgcgggg                                                                                                 >  2:216863/3‑238 (MQ=255)                                                                                                                                                         tgcAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCTTTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTc                                                                          <  1:298688/249‑1 (MQ=255)                                                                                                                                                         tgcAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACGCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTcc                                                                         <  2:305872/250‑1 (MQ=255)                                                                                                                                                               cagcTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCg                                                                  <  1:35118/251‑1 (MQ=255)                                                                                                                                                                                     ccGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGGCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCt                                            <  2:350044/251‑1 (MQ=255)                                                                                                                                                                                      cGCTGGCGCGGGGAACTCAAGCTGTCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTg                                           <  1:279876/251‑1 (MQ=255)                                                                                                                                                                                            cgcgGGGAACTCAAACTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTCTCCTCGCTGGCGCGGGGAACTCTCGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAAGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGTCCCAAACGGTTTATCCCCGCTGGCGCgg                                     >  2:293120/1‑251 (MQ=255)                                                                                                                                                                                                                 ggcAATCTCTTTCGGGGTCCGTCCGGTTTATCCTCCCTCGCGCGGGGCACTCTAGTTTACGTCTCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTCGCGCGGGGACCTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCACAAATTCCAGACCCGATCCAATCGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAAt                >  2:38420/1‑251 (MQ=255)                                                                                                                                                                                                                             cGGGGTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACa     <  2:26399/250‑1 (MQ=255)                                                                                                                                                                                                                              ggggTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAgg   >  1:31470/1‑251 (MQ=255)                                                                                                                                                                                                                               gggTGAGTCCGGTTTATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAggg  <  2:103764/251‑1 (MQ=255)                                                                                                                                                                                                                                             tATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGAccc                                                                   >  2:193020/1‑172 (MQ=255)                                                                                                                                                                                                                                             tATCCTCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGAccc                                                                   <  1:193020/172‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                        CTAACGCGGGGAACTCGTAGTCCATCATTCCACCTATGTCTGAACTCCCGGTTTATCCCCGCTGGCGCGGGGAACTCCCGGGGGATAATGTTTACGGTCATGCGCCCCCCGGTTTATCCCCGCTGGCGCGGGGAACTCTGGGCGGCTTGCCTTGCAGCCAGCTCCAGCAGCGGTTTATCCCCGCTGGCGCGGGGAACTCAAGCTGGCTGGCAATCTCTTTCGGGGTGAGTCCGGTTTATCCCCGCTGGCGCGGGGAACTCTAGTTTCCGTATCTCCGGATTTATAAAGCTGACGGTTTATCCCCGCTGGCGCGGGGAACTCGCAGGCGGCGACGCGCAGGGTATGCGCGATTCGCGGTTTATCCCCGCTGGCGCGGGGAACTCGCGACCGCTCAGAAATTCCAGACCCGATCCAAACGGTTTATCCCCGCTGGCGCGGGGAACTCTCAACATTATCAATTACAACCGACAGGG  >  NC_000913/2877836‑2878308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑