Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,288	C→T	G161D (GGC→GAC)	recA ←	DNA recombination/repair protein RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,288	0	C	T	100.0%	163.9 / NA	44	G161D (GGC→GAC)	recA	DNA recombination/repair protein RecA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/22);  total (22/22)

CGCTACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCA  >  NC_000913/2823046‑2823531                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    ggCTACCCACCACGGTTTCGCCCTCTTGCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACCCCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTGGGACGGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCg                                                                                                                                                                                                                                             <  2:355384/250‑1 (MQ=255)         accacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGAt                                                                                                                                                                                                                                     >  1:166488/1‑251 (MQ=255)          ccacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGAtt                                                                                                                                                                                                                                    >  2:88954/1‑251 (MQ=255)              gTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATCCGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCg                                                                                                                                                                                                                                <  2:298338/251‑1 (MQ=255)                  tCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACCCCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCtt                                                                                                                                                                                                                             <  1:340336/250‑1 (MQ=255)                                  cgcCGATACGACGGATGTCGAGACGAACAGCGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTgccgcc                                                                                                                                                                                                            >  1:333646/1‑251 (MQ=255)                                    ccGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCAc                                                                                                                                                                                                          >  2:300242/1‑251 (MQ=255)                                                 ggTCGAGACGAACTGAGGCCTAGAATCTCTGCGCGTTACCACCGGTTGTGGTTTCCGGGTTACCGACCTTCCCACCAATGTTCATACGGATCTGGTTGATGAAGATCAGCTGCGTGTGGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGACAacgacg                                                                                                                                                                                             <  2:81913/250‑1 (MQ=255)                                                     gagaCGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATaa                                                                                                                                                                                         <  1:360193/251‑1 (MQ=255)                                                     gagaCGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATa                                                                                                                                                                                          >  2:78430/1‑250 (MQ=255)                                                        aCGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGt                                                                                                                                                                                      <  2:312806/251‑1 (MQ=255)                                                         cGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTc                                                                                                                                                                                     >  1:221401/1‑251 (MQ=255)                                                         cGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTc                                                                                                                                                                                     >  2:125135/1‑251 (MQ=255)                                                          gAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCt                                                                                                                                                                                    >  1:167583/1‑251 (MQ=255)                                                                         aTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAAcgc                                                                                                                                                                     >  1:324832/1‑251 (MQ=255)                                                                          tttCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAAcgcg                                                                                                                                                                    >  1:167684/1‑251 (MQ=255)                                                                           ttCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTCCTGCGCCAGAACgcgc                                                                                                                                                                   >  1:55133/1‑251 (MQ=255)                                                                              aGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGcc                                                                                                                                                                  >  1:350906/1‑249 (MQ=255)                                                                                      accaccGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGTTCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTca                                                                                                                                                         <  1:357275/250‑1 (MQ=255)                                                                                        caccGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTcac                                                                                                                                                        >  1:143421/1‑249 (MQ=255)                                                                                                          ggTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCg                                                                                                                                    <  2:277906/251‑1 (MQ=255)                                                                                                                                         gATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGt                                                                                                     >  2:144368/1‑251 (MQ=255)                                                                                                                                                   atgaAGATCAGCCGGGTGTTGGACGGCTTCAGGTTACCCGCCAGCTTACGCCTCGCCTGGCTCATCATTCGGTCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCTCAGCAGGTTGTCTATATCGAc                                                                                           <  2:113762/251‑1 (MQ=255)                                                                                                                                                          tCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAg                                                                                     <  1:283224/250‑1 (MQ=255)                                                                                                                                                               agcGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACg                                                                               >  2:106792/1‑251 (MQ=255)                                                                                                                                                                    gtTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGt                                                                          <  1:125135/251‑1 (MQ=255)                                                                                                                                                                      tgaaCTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTa                                                                         <  2:172704/247‑1 (MQ=255)                                                                                                                                                                      tGGACTGCTTCAGGATACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGGGCCGCAAGGACCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCCCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACCGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGt                                                                          >  2:169776/1‑249 (MQ=255)                                                                                                                                                                                     tACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGcgcg                                                         >  1:197137/1‑251 (MQ=255)                                                                                                                                                                                       cccGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCgtg                                                       >  2:9790/1‑251 (MQ=255)                                                                                                                                                                                                 tACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATc                                              <  1:275897/250‑1 (MQ=255)                                                                                                                                                                                                   cGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGAt                                           <  2:324832/251‑1 (MQ=255)                                                                                                                                                                                                      aTCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATaa                                         >  2:145873/1‑250 (MQ=255)                                                                                                                                                                                                        cGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACg                                      >  1:70191/1‑251 (MQ=255)                                                                                                                                                                                                        cGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACg                                      <  1:127380/251‑1 (MQ=255)                                                                                                                                                                                                          ccTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGca                                    <  1:88954/251‑1 (MQ=255)                                                                                                                                                                                                             ggCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGcac                                   <  2:360147/249‑1 (MQ=255)                                                                                                                                                                                                                     atACGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTGCGATTTCCGCGTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTAcc                         <  2:167684/251‑1 (MQ=255)                                                                                                                                                                                                                        cGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTc                      <  1:106792/251‑1 (MQ=255)                                                                                                                                                                                                                        cGTGCCGCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTc                      <  2:167583/251‑1 (MQ=255)                                                                                                                                                                                                                            ccGCAAGTCCCATGTGAGAGTCGTCGATTTAGCCTTCGCTTTTCGCTTCCGGCCACAGTGCAGCAAAGAAGTCAACGAAGATAACGACTCCTGCGCAAGAACGCGCCATGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTATCCGGCTGGGAGCACAGAAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGATCAAGCGCGAGTTCAGCATCGATAAACGCACAGGCTTTACCTTCAcgc                  >  1:339358/1‑251 (MQ=255)                                                                                                                                                                                                                              gCAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAcgctg                <  2:305676/251‑1 (MQ=255)                                                                                                                                                                                                                               cAAGGCCCATGTGAGAGTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAcgctgc               >  1:230447/1‑251 (MQ=255)                                                                                                                                                                                                                                            agagTCGTCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCa  <  2:65744/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    CGCTACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATACGGATCTGGTTGATGAAGATCAGCAGCGTGTTGGACTGCTTCAGGTTACCCGCCAGCTTACGCATCGCCTGGCTCATCATACGTGCCGCAAGGCCCATGTGAGAGTCGCCGATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCA  >  NC_000913/2823046‑2823531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑