Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,850	G→A	noncoding (11/77 nt)	argQ ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,850	0	G	A	93.9%	93.3 / ‑6.2	33	noncoding (11/77 nt)	argQ	tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (7/24);  total (8/25)
Fisher's exact test for biased strand distribution p-value = 4.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTC  >  NC_000913/2817610‑2818094                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      ttAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATg                                                                                                                                                                                                                                              <  1:33581/250‑1 (MQ=255)            atatTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAAc                                                                                                                                                                                                                                  <  1:117750/251‑1 (MQ=255)            atatTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAAc                                                                                                                                                                                                                                  >  1:263390/1‑251 (MQ=255)                     aaGGAAATTTTTTGGAGAAGATGGGGCATCCGGGAGGATTACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTAc                                                                                                                                                                                                                         <  2:242955/251‑1 (MQ=255)                          aaTTTTTTGTAGAAGATGGTTGATCCGGGAGGATGACTCACTGGCGTGCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTGATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCTGGAAAACTTATTCTActgctg                                                                                                                                                                                                                    <  1:254485/251‑1 (MQ=21)                                  ggAGAATATGGTGGAGCCGGGAGAATGACTCACTGTCGTTCGCCCTTCGGGCCGTTGCTGGAGCAACGTTATCCTTCCTGGTGCCTGCGGTGAATACCGCTATCTCTTCGATTACTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATGCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGAt                                                                                                                                                                                                            <  2:101989/251‑1 (MQ=18)                                  ggAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGAt                                                                                                                                                                                                            <  1:175816/251‑1 (MQ=255)                                     gaagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATAcc                                                                                                                                                                                                         <  1:216407/251‑1 (MQ=255)                                      aagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGCTACCTTCATTGCTGAAGGTTCCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTAATCTACCCCGCTGAGCTACGAATGCATCGGGAGAACtgctttatatgctcaacttccacccc                                                                                                                                                                                                        >  2:55332/1‑226 (MQ=18)                                                                              cTTCGGGCCGTTGCTGTCGCAACTTTCTCCTTCCTTTTGCCTGCGGCTACTACCGCTACCTCTTCGATACCTTCATTGCTGCAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCCAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAACACTTCTTCTACTCCTGATATTGATACCGCTACTCAACCCATATCAAATAAGCGATgacacatccggg                                                                                                                                                                 >  1:229555/1‑239 (MQ=14)                                                                                 cGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggagg                                                                                                                                                              <  1:185377/250‑1 (MQ=255)                                                                                             ggCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                                                 <  1:40900/251‑1 (MQ=255)                                                                                                   aCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTc                                                                                                                                           <  1:95813/251‑1 (MQ=255)                                                                                                          ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                                                    <  1:32268/251‑1 (MQ=255)                                                                                                          ccttcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTc                                                                                                                                    <  2:259809/251‑1 (MQ=255)                                                                                                               cgggtgccgacGGTTAATACGGCTATATCGTCGATATCTTTATTGCTGAAGGGGACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACGCAATCCAGCGGAACTGCGGATGCATCGGGAAAACTTATACTACTGCTGATATTGATACCGCATCTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGAATTACTCGCGTTGAGGTTCGACCTACGGGcc                                                                                                                               <  2:322703/241‑1 (MQ=17)                                                                                                                          ggTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGGTGCT‑aaaac                                                                                                                   >  1:185729/1‑249 (MQ=255)                                                                                                                                   cGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTTTACTGCTGTTTTTGATACCGCTACTAACGCCAGATCAATTAAGAGGGGATGCATCCGGATGGATTACTCGCCTGGCGGCTTACCCTTCAGGGCGTTGGT‑AAAGCACAGTTatc                                                                                                          >  2:325230/1‑251 (MQ=14)                                                                                                                                     cTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGTAGTGTATCCAGATGGACTACGGATGCATCGGGAAAACTTCTTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCTCCCTTCGGGCCGGTGCTCAAAG‑AACGTTatcat                                                                                                        >  2:129965/1‑251 (MQ=18)                                                                                                                                            ttCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGt                                                                                                 >  1:54350/1‑251 (MQ=255)                                                                                                                                                aTACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCt                                                                                             <  1:86879/251‑1 (MQ=255)                                                                                                                                                   ccTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCg                                                                                          <  1:57110/251‑1 (MQ=255)                                                                                                                                                          tGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGAGAAAACTTATTCGACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAAt                                                                                    <  2:320013/250‑1 (MQ=255)                                                                                                                                                                 ggTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCACCTGCTGATATTGATACCGCAACTAAAGCCCTATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAAAGTTTTCAAACCCAGTGCCTGCGGGTAATTTCGCTa                                                                             >  1:143881/1‑250 (MQ=17)                                                                                                                                                                                          ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAg                                                   <  1:305515/251‑1 (MQ=255)                                                                                                                                                                                          ggaggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCGGAAg                                                   <  1:248846/251‑1 (MQ=255)                                                                                                                                                                                                      ccTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAAATACGGATGCATCGGGAAAACTTATTCGACTGCTGAAATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAat                                       <  2:354604/251‑1 (MQ=255)                                                                                                                                                                                                                           gacgtattctctaagcgacTGAACTACGGATACATCGGGAAAACATAATCTACTGCTGATATTAATACCACTACTACAGCGATAGCAATTAAGGGATGGGGCCACCGCGAGAAGTTCTCGCATTGCGGCTCGCCCTTCGGGCAGTTGCT‑GAAGCAACGTTATCATCCCTGGTGCCAGCGGTTAATTCCGCTGCCTCTTCGATAGCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTc                  <  2:6503/233‑1 (MQ=16)                                                                                                                                                                                                                                       aTCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAcc      <  1:109915/251‑1 (MQ=255)                                                                                                                                                                                                                                       aTCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc       <  2:54350/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAc       <  1:118979/250‑1 (MQ=255)                                                                                                                                                                                                                                         ccAGTTGAGCGAAGGATGCATAGGGAAAACTTATTCTACTGCTTATATAGATACAGCTATTAAAGCCATATCATTTAAGGGATGGTGCGTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATTCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGAcc      <  2:125348/249‑1 (MQ=21)                                                                                                                                                                                                                                           aGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTc  <  1:129965/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCT‑AAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTC  >  NC_000913/2817610‑2818094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑