Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,756,976	G→A	R273Q (CGA→CAA)	intA →	integrase IntA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,756,976	0	G	A	100.0%	99.2 / NA	27	R273Q (CGA→CAA)	intA	integrase IntA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/13);  total (14/13)

ATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTAT  >  NC_000913/2756729‑2757183                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                attgatGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAAt                                                                                                                                                                                                              <  1:253105/251‑1 (MQ=255)      tGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGa                                                                                                                                                                                                            >  2:11410/1‑248 (MQ=255)             ccTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAAcc                                                                                                                                                                                                   <  1:52870/250‑1 (MQ=255)                aGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAAGGAAGATGAACCGGGa                                                                                                                                                                                               <  1:266033/251‑1 (MQ=255)                      aaTATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATAc                                                                                                                                                                                         >  1:3005/1‑251 (MQ=255)                                           aaaCCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGc                                                                                                                                                                    >  1:144627/1‑251 (MQ=255)                                           aaaCCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGc                                                                                                                                                                    >  2:159279/1‑251 (MQ=255)                                                         aaaCATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTgg                                                                                                                                                      <  2:282977/251‑1 (MQ=255)                                                             aTGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGTTGGGGCTCTTGCTATTCTGGAAat                                                                                                                                                  >  2:262834/1‑251 (MQ=255)                                                                       tCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTCACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGCTGGGGCTCTTGCTATTCTGGAAATGATGAAGCct                                                                                                                                        >  1:225857/1‑251 (MQ=255)                                                                                                     acacCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCt                                                                                                          >  1:306873/1‑251 (MQ=255)                                                                                                          aTGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCg                                                                                                     <  1:11410/251‑1 (MQ=255)                                                                                                                 cGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTg                                                                                                                                                               <  1:27562/186‑1 (MQ=255)                                                                                                                 cGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTg                                                                                                                                                               >  2:27562/1‑186 (MQ=255)                                                                                                                  ggCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGc                                                                                             <  1:256979/251‑1 (MQ=255)                                                                                                                         aTCAACATGTCCACAAGAGGCCGGTTCAGGTGGCCACTTCTAACCATCTCCC‑CCCCTGCCGAAGCTGCTGAGGCTCGAGGGGAGGAGATCGATTTCAAGGCTATGGAATGGAAAATTCCTGCATCTCAAATTAAGATGAACCGGGACCATACGGTTCCACTATCGGATTGGGCTCTTGCTATTCTGGAAATGATAAAGCCTCTCATTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCccaacca                                                                                      <  2:138085/251‑1 (MQ=255)                                                                                                                                ggTCCACAAGATGCCTGTTCATGGGGCAACTTCTAACCACCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATg                                                                               <  2:256233/250‑1 (MQ=255)                                                                                                                                                gTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGt                                                                 <  2:144627/249‑1 (MQ=255)                                                                                                                                                    aTGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATgc                                                           >  2:59681/1‑251 (MQ=255)                                                                                                                                                              ttCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCa                                                   >  2:309760/1‑249 (MQ=255)                                                                                                                                                               tCTAACCA‑CACCCCGCCCTGCCGAAGCTGCGGAGGCTCGATGGGATGAGATCGATTGCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGTTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAg                                                 <  2:3005/250‑1 (MQ=255)                                                                                                                                                                      aTCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTgg                                         >  1:196885/1‑251 (MQ=255)                                                                                                                                                                                  tGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGgtg                              >  2:234202/1‑251 (MQ=255)                                                                                                                                                                                    ccGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGgtgt                             >  1:234409/1‑250 (MQ=255)                                                                                                                                                                                                         gggATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGt       <  1:309760/251‑1 (MQ=255)                                                                                                                                                                                                           gATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATGCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTc     <  2:340546/251‑1 (MQ=255)                                                                                                                                                                                                              gagaTCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCAAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTAt  >  2:58912/1‑251 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                ATTGATGCTGTTCCTAGTGTAAATATCGGAAAAGCTTTCGAGAAACCGCAAAAGAAAAACATGCCAAGCATCCGGCCGGATCAACTTCCGCAGCTAATGCACACCATGCGTACGGCAAGTATCAGCATGTCCACAAGATGCCTGTTCATGTGGCAACTTCTAACCATCACCC‑GCCCTGCCGAAGCTGCTGAGGCTCGATGGGATGAGATCGATTTCAATGCTAGCGAATGGAAAATTCCTGCAGCTCGAATGAAGATGAACCGGGACCATACGGTTCCACTATCTGATGGGGCTCTTGCTATTCTGGAAATGATGAAGCCTCTCAGTGGTGGCCGAGAATTTATCTTTCCTAGCCGTATCAAGCCCAACCAACCAATGAATAGCCAAACAGTGAATGCAGCACTCAAGCGTGCTGGCTTAGGAGGTGTACTTGTTTCACACGGCTTGCGTTCTAT  >  NC_000913/2756729‑2757183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑