Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,396	G→A	A145A (GCG→GCA)	ypjD →	cytochrome c assembly family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,396	0	G	A	100.0%	105.8 / NA	30	A145A (GCG→GCA)	ypjD	cytochrome c assembly family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/19);  total (11/19)

GCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATT  >  NC_000913/2748152‑2748624                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     gCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCa                                                                                                                                                                                                                                 >  1:142883/1‑250 (MQ=255)                      tGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACAGTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCa                                                                                                                                                                                                            <  2:55311/250‑1 (MQ=255)                                  tgatCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACa                                                                                                                                                                                                 >  1:292692/1‑249 (MQ=255)                                        gTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGc                                                                                                                                                                                           <  1:198215/249‑1 (MQ=255)                                        gTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGc                                                                                                                                                                                           <  2:142883/249‑1 (MQ=255)                                             gTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGtt                                                                                                                                                                                    <  1:251157/251‑1 (MQ=255)                                                aTGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTaa                                                                                                                                                                                 <  2:316046/251‑1 (MQ=255)                                                          tgGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGc                                                                                                                                                                                                                                  >  1:133524/1‑192 (MQ=255)                                                          tgGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGc                                                                                                                                                                                                                                  <  2:133524/192‑1 (MQ=255)                                                                             ctggctgCTGCAACCCATTGCCTTTGCTTTCGCGCTTATCAACCTGGCGCGGGCAAACTTCATGCCCAATGAATACATCACCCATCTGGACGATAAGCCGAGGATGCTGGGGCACCTTGGCCTATCGCTCTTTTCCTATGCCACGCTAATCATAGCCGCCCTGTACGCACTGCAACTGGCGCGGCTTGATAACCACATGAAGAAGAAGAAGCTGGCGTTTAACCAGGACATGCCGCAATTGATGAGTTTCg                                                                                                                                                    >  2:223681/1‑251 (MQ=255)                                                                               ggctgCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAg                                                                                                                                                  <  2:70183/251‑1 (MQ=255)                                                                                   gctgctACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACGGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCg                                                                                                                                                <  2:356486/249‑1 (MQ=255)                                                                                                    aTGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCa                                                                                                                               <  1:307386/249‑1 (MQ=255)                                                                                                    aTGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGGTCCACATCAc                                                                                                                              <  1:140109/250‑1 (MQ=255)                                                                                                      gCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGAGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGc                                                                                                                            >  2:178682/1‑250 (MQ=255)                                                                                                       ccTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGc                                                                                                                            >  1:353982/1‑249 (MQ=255)                                                                                                           tGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGAtt                                                                                                                       <  1:178682/250‑1 (MQ=255)                                                                                                            gcgcTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCGTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTgg                                                                                                                     <  2:270791/251‑1 (MQ=255)                                                                                                               cTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACCTCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGCGCGTAAACTGTTCCACATCACGCCGATTGGctt                                                                                                                  >  2:333521/1‑249 (MQ=255)                                                                                                                  aTCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCgtggt                                                                                                               <  2:353854/251‑1 (MQ=255)                                                                                                                                        tCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCttt                                                                                           <  1:13709/249‑1 (MQ=255)                                                                                                                                        tCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGAGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTGTAACCAGGAAATGCCGCCATTGATGAGTATCGGGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCttt                                                                                           <  1:338653/249‑1 (MQ=255)                                                                                                                                                                       aGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTa                                                          <  2:353982/251‑1 (MQ=255)                                                                                                                                                                                   gATGCTGGTGCACATTGGCTTATCGATCTTTTCCTATGCCAAGCTAATTATAGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAAGGCGCACGATTTGCACTGGCCTGTTGTACATGCACAACTTATTTAACAttgacact                                               >  2:295265/1‑243 (MQ=255)                                                                                                                                                                                             cacaTTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGc                                    >  2:291079/1‑251 (MQ=255)                                                                                                                                                                                                   ggCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGACGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGct                              >  2:252485/1‑251 (MQ=255)                                                                                                                                                                                                    gCTTATCTCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACACCTTATTTAGCATGGACAATATCGACAAGGCTGTGctc                             >  1:14505/1‑251 (MQ=255)                                                                                                                                                                                                       tATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGctctc                           <  2:306036/250‑1 (MQ=255)                                                                                                                                                                                                                  tCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACGTCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtggcgt                <  1:291079/250‑1 (MQ=255)                                                                                                                                                                                                                                aaTTATCGTCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCGTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATAtt  <  1:354869/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                     GCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTGCACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATT  >  NC_000913/2748152‑2748624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑