Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,700,747	G→A	L84L (CTC→CTT)	acpS ←	holo‑[acyl‑carrier‑protein] synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,700,747	0	G	A	100.0%	89.8 / NA	28	L84L (CTC→CTT)	acpS	holo‑[acyl‑carrier‑protein] synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

TCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTA  >  NC_000913/2700505‑2700988                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  tCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCtcatc                                                                                                                                                                                                                                           >  1:354824/1‑251 (MQ=255)                  tgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGTGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCTCTCATCTGCCAGCGTTACATGCATATTTGCAACTCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCTTAACCGTGGTTTTCCAAGCTCATCATTGAATACTTCAAAt                                                                                                                                                                                                                           <  2:171780/250‑1 (MQ=255)                  tgAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTAGTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCGCATCTGCCAGCGTTACAGGCATATTTGCAACGCCCAGCTTTTCCGCCAGTGTTAATGCCGCGCGCCATAGCCGTTGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAAt                                                                                                                                                                                                                           <  1:141645/250‑1 (MQ=255)                         gCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTaaa                                                                                                                                                                                                                   <  2:290693/251‑1 (MQ=255)                           aTGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACg                                                                                                                                                                                                                 >  2:24392/1‑251 (MQ=255)                                         tAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTTCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCgg                                                                                                                                                                                                   >  1:203177/1‑251 (MQ=255)                                               tAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCg                                                                                                                                                                                             >  1:34908/1‑251 (MQ=255)                                                           cccccGTTCCTTACCAGTTCGGGGGTTTTACTTTTTACAGAGAACGGTATTATTTTTAACTTTCAATAATTACAGTGGCACAAGCATAGAGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACgtt                                                                                                                                                                                 >  1:208887/1‑249 (MQ=255)                                                              ccGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCtttt                                                                                                                                                                              <  1:27719/251‑1 (MQ=255)                                                                          aGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTTCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCCTTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGTTGCCAACCGCTTTTGCTGCGGcttct                                                                                                                                                                  >  2:232496/1‑251 (MQ=255)                                                                                ggggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACTCTTTTGCTTCGGCTTCTGTcaca                                                                                                                                                            <  2:51722/251‑1 (MQ=255)                                                                                           ttttAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCtt                                                                                                                                                 >  2:181322/1‑251 (MQ=255)                                                                                                aaGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGACAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGcc                                                                                                                                             >  1:264903/1‑250 (MQ=255)                                                                                                           attattTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCAcc                                                                                                                                 <  2:312077/251‑1 (MQ=255)                                                                                                                           aataatTACCGTGGCACAAGCATAGTGCCGCTCATCTGACAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCAGTAGCCGTGGTTTGCCAAGCTCATCATTGCATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCTCCGGCTGGTGGTGCGttt                                                                                                                 >  1:324458/1‑251 (MQ=255)                                                                                                                                            aaGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCg                                                                                                <  1:324117/251‑1 (MQ=255)                                                                                                                                               cATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTa                                                                                             <  2:354824/251‑1 (MQ=255)                                                                                                                                                    tGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGCGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCt                                                                                        >  1:207210/1‑251 (MQ=255)                                                                                                                                                          tCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATAc                                                                                  >  1:19056/1‑251 (MQ=255)                                                                                                                                                              cTGCCCGCGTTACATGCATATTTGCACCGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCACCATAGCCGTAGCCGTGGTTTCCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAACACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACgcgg                                                                              >  2:334560/1‑251 (MQ=255)                                                                                                                                                                  cAGCGTTACATGCATATTTGCACCGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTg                                                                          >  1:311184/1‑251 (MQ=255)                                                                                                                                                                     cGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCa                                                                       <  1:24392/251‑1 (MQ=255)                                                                                                                                                                                               gCTTTTCCGCCAGGTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGAGCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCATCGGCTGGTGGTGCGTTTTTCAGATAGCCCATTCGTTATCGCTTAATACGCTTCGGGCCAGTCTATCACCGGACCGGGCGATCAcc                                             <  2:334005/251‑1 (MQ=255)                                                                                                                                                                                                            ttttAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCATAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCTGCTTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcg                                  >  1:305196/1‑249 (MQ=255)                                                                                                                                                                                                             tttAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGAGCGGGCGATCACCGCCTCGATgcgagc                               >  1:30196/1‑251 (MQ=255)                                                                                                                                                                                                                 aTGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTTATTAAACGCCAGACCAGTGCGGATCCCGGTGCCAAACGCTGTTGTTGCGGCTTCTGTCACAGCAAAACGCTTCGTCAGAAAACGCACCGGCTGGTGGTTCGTTGTCCATATAGCTCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCAGCGGATCGGGCGATCACCGCTCCGATGCTAGCGAtc                           <  2:331686/251‑1 (MQ=255)                                                                                                                                                                                                                             aTAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATcc               <  2:118380/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTa  >  2:144659/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  TCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTA  >  NC_000913/2700505‑2700988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑