Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,930,0470GT26.1% 79.4 / 11.9 46G158C (GGC→TGC) sdaBL‑serine deaminase II
Reads supporting (aligned to +/- strand):  ref base G (14/20);  new base T (12/0);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 3.64e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCT  >  NC_000913/2929806‑2930295
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          
cGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGGTGCTTTTTCGTTGTTGAAAAGCACTTTGGTcagcacg                                                                                                                                                                                                                                                   >  2:115498/1‑249 (MQ=255)
  tGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATTGTTGATGAAGAGCATTTTTGcccgccgga                                                                                                                                                                                                                                                  >  1:225398/1‑243 (MQ=255)
   ggATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGAt                                                                                                                                                                                                                                                 >  1:7140/1‑250 (MQ=255)
                aTCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGa                                                                                                                                                                                                                                   <  1:152684/251‑1 (MQ=255)
                       gTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTCGTTTGTGAAAAGCACTTTGGGCCGCAGGGTAGCGCACCgggtgttggtcct                                                                                                                                                                                                                            >  2:17739/1‑240 (MQ=255)
                                aGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTCTGTGTTGAAGAGCCTTTTTGCCCGCAGGAGAGCGCCCCGGGTGGAGGTCCTTTTcccta                                                                                                                                                                                                                    >  2:22250/1‑247 (MQ=255)
                                   aTGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGt                                                                                                                                                                                                                <  1:220260/251‑1 (MQ=255)
                                     gtgAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGtt                                                                                                                                                                                                               <  2:53274/250‑1 (MQ=255)
                                     ggtaataatcagggtctccgaaTGCTGGCAAACGGTAAGCATGAAGTGGAGTCCCCGGTTGAGCAGTGCATGAACTTCCACGCCGGCAACCGTTCTCTGCATGAAAACGGAATGCGCATTACCGCGCTGGCGGGCGATGAAGTCGTTGACAGCCAGACTTACTACTCTATGAGGGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGAGCACCGGTTGAAGTTCCTTATCCGTACAggtc                                                                                                                                                                                                              <  2:188289/230‑4 (MQ=255)
                                       gAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTTGTTTATGAAGAGCACTTTTGCCCGCAAGGTAGCGCACCGGTTGAAGTTCCTTATCCGTACACTTctc                                                                                                                                                                                                            >  2:204882/1‑249 (MQ=255)
                                              cATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGcc                                                                                                                                                                                                       <  1:199813/249‑1 (MQ=255)
                                                    cGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCa                                                                                                                                                                                               <  1:219574/251‑1 (MQ=255)
                                                     gCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTTATGAAGAGCATTTTGGCCAGCCGGATAGCGCACCGGGTGGAGTTCCTTATCCGTAAAGTTCAGCAGGCGATCttca                                                                                                                                                                                               >  1:286840/1‑247 (MQ=255)
                                                      ccTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCaaa                                                                                                                                                                                             <  1:275123/251‑1 (MQ=255)
                                                        tGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTTGCTTTTTCGTTGATGAAGAGCATTTTGGCCCGCAGGATAGCGCACCGGGTGAAGGTCCTTATCCGTACAGTTCAGCAGCCGATCTGCaaaa                                                                                                                                                                                            >  1:67502/1‑250 (MQ=255)
                                                            gcgggCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACAtt                                                                                                                                                                                       <  2:337954/248‑1 (MQ=255)
                                                                   aaCGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTCGTTGATGAAGAGCATTTTGGGCAGCAGGATAGCGCACCGGTTGAAGGTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAgg                                                                                                                                                                                 >  1:197242/1‑250 (MQ=255)
                                                                             aTGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGGGGCTTTATAGGTGGTTCAGAACATTTTGGCCAGCAGGGAAGCCCCCCGGTTTAAGTTCCTTTTCCGGACAAGTCACCACCCGAACTCCAAAAACAATGTTAGGGAA‑CCggggcg                                                                                                                                                                     >  2:307814/1‑248 (MQ=255)
                                                                                       gTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACCACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATTGTTGGTGGAAAACATTTTTGGCAGCAGGATAGCGCACCGGTTTGAGTTCCTTATTCGGACAGGTCCGCGGGCGGACTGCAAAAACATTGTCCAGAAA‑ACGGGcctgccacctctg                                                                                                                                                           >  2:205758/1‑239 (MQ=255)
                                                                                         tCCCGGTTGAGCAGTGCATGAACTGCCACGCCGACAACCTTGCTCTGCATGAAAACGGTAGGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGTCGGTGGCTTTATCGTTGATGAAGATCATTTTGGCCATCAGGATAGCGCACCGGTTGAAGTTCCTTATCCTTTCAGTTCAGCAGCCGATGTGTAAAAACATTGTCACGAAA‑CTGTGCGGTCACTCTC‑Tgg                                                                                                                                                         <  2:182863/250‑1 (MQ=255)
                                                                                          cccGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAAAGCATTTTTGCCAGCAGGATAGCGCACCGGTTGAAATTCCTTTTCCGTACAGTTCAGCAGCCGCACTGCAAAAACATTTTCAGGAAA‑ACGGGCTGTCACTCTC‑TGGcc                                                                                                                                                       >  1:216102/1‑251 (MQ=255)
                                                                                            cGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCtg                                                                                                                                                     <  1:171800/251‑1 (MQ=255)
                                                                                                         cATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACAGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACg                                                                                                                                        <  1:99468/251‑1 (MQ=255)
                                                                                                                              ccTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTTGCCAGCAGGATAGCGCACCCGGTGAAGTTTCTTTTCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TTGCCCGGTGATGAAAAACGAGCTGGCGCTGCACAGCaaa                                                                                                                    >  1:281140/1‑250 (MQ=255)
                                                                                                                                  tctcTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAaagaag                                                                                                                <  1:70635/250‑1 (MQ=255)
                                                                                                                                      tGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGGTTTTTTGTTTATGAAGAGCATTTTTGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTTAGCAGCCGCTCTGCAAAAACCTTTTCCGGAAA‑CCCGGCTTTCACTCTC‑TTGCCTGTTGGTGAAAAACGAGCTGGCGCTGCCCAGCCAAGAAAAGCTg                                                                                                           >  2:108555/1‑251 (MQ=255)
                                                                                                                                       gCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTCGTTGATGAAGGGGCTTTTGGCCAGCATGGTAGGCCACCGGTTTAAGTTCCTTATTCGTACAGATCAGCCGCCGATCTGCCAAAACATTGGCAGGAAA‑ACGGGCCGTCACTCCC‑TTGCCTGATGATGGAAAAAGAGGTGGAGCTGCCCAGCAACGCAGAGCTgg                                                                                                          >  1:1073/1‑251 (MQ=255)
                                                                                                                                              aaCGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGGTTTTTTGTTTCTGAAAAGCATTTTTGGCAGCAGGGTAGCGCCCCCGGTGGCGTTCCTTTTTCCTACAAGTCAGCAGCCGGTTTGCCAAAAAATTGTTAGGAAA‑CCCGGGTGTCACACTCCTGGCC‑GAAGGTGAAAAAAAAACCGGCGCTGcacacccaaaaaaagccggaacaac                                                                                                    >  2:340574/1‑229 (MQ=255)
                                                                                                                                                 ggtatgctgatgacggcgatggggggcgATAAAGTCGTTTACAACCAGACATACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCAGGCAGGATATCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGGCAGGACA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGCGCTGGCGCTGCACAGCAAAGAAGAGCTGGAAAAGCACCt                                                                                                <  2:229120/228‑1 (MQ=255)
                                                                                                                                                    aTGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTTTCGTTGATGAAGAGAATTTTTGCCAGCCGGGTAGCGCACCGGTTGGAGTTCCTTATCCGTACAGTTTAGCAGCCGATCTGCCAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCCCAGCaacacaaagctgggagtgcacctggg                                                                                             >  1:49371/1‑227 (MQ=255)
                                                                                                                                                        gcATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCCGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCCGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGCTGATGAAAAACGAGGTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCggac                                                                                         >  1:20742/1‑248 (MQ=255)
                                                                                                                                                               cgcgCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGGTTTATCGTTGATGAAGAGACTTTTTGCCAGCAGGATAACGCACCGGGTGGAGTTTCTTATTCGTACAGTTCAGGAGCCGGTCTGCAAAAACATTGTCAGGGAA‑CCGGGCTGTCACTCTC‑TGGCCCGATGATGAAAAACGAGCTGGCGCTGCACCGCAAAGAAAAGCTGGGAAAGCACCTGGCGGACggcttgg                                                                                  >  1:211743/1‑245 (MQ=255)
                                                                                                                                                                          gcgATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATgcgc                                                                       <  1:71466/251‑1 (MQ=255)
                                                                                                                                                                                      tttACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTTGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTTTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCCCAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTg                                                             >  1:86157/1‑249 (MQ=255)
                                                                                                                                                                                           aGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGGATTGAGCGCgg                                                      >  1:115209/1‑251 (MQ=255)
                                                                                                                                                                                            gCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATTAAGAGAATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTTTCCGTACAGTTCAGCAGCCGCTCTGCCAAAACATTGTCAGGAAA‑CCGGGCCGTCAATCTC‑TGGCCTGATGATGAAAAACGAGCCGGCGCTGCACAGCAAAGAAGAGCTGGGACAGCACCTGGAGGACGTCTGGGAAATCATGCGCGGGGGGTTTGAGCGCggg                                                     >  1:200707/1‑250 (MQ=255)
                                                                                                                                                                                               aGACTTACTACTCTATTGGCGGTGGCTTTATCGTTTATGAAGAGCATTTTGGGCAGCAGGATAGCGCACCGGGTGAAGTTCCTTATCCGTAAAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGGGCTGCACAGCAAAGAAGAGCTGGAACAGCAACTGGCGAACGTCTGGGAAGTCATGCGCGGCGGGATTGAGCGCGGGAtt                                                  >  1:360262/1‑251 (MQ=255)
                                                                                                                                                                                                   ttACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTTGCCAGCAGGATAGCGCCCCCGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCCCTCTGCAAAAACATTGTCCGGAAAACCGGG‑TGGCCCTCTC‑TGGCCTGATGATGAAAAACGAACTGGGGCTGCACCGCAAAGAAAAGCTGGGACAGCACCTGGCGAACGTCTGGGGAGTCCTGCGcggcgggttttagggcggggtttccc                                              >  2:258960/1‑231 (MQ=255)
                                                                                                                                                                                                     actgctctagttgCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCAcc                                            <  2:319277/239‑1 (MQ=255)
                                                                                                                                                                                                     actactCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCAc                                             <  1:251907/250‑1 (MQ=255)
                                                                                                                                                                                                                      ggCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTgg                           <  1:27251/251‑1 (MQ=255)
                                                                                                                                                                                                                       gCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGc                          <  1:115498/251‑1 (MQ=255)
                                                                                                                                                                                                                         tttATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCa                         >  1:190330/1‑250 (MQ=255)
                                                                                                                                                                                                                                ttGATGGAGAGCCTCTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTAACCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCCGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATGTCCCCCGAAGGCGTGTTGCCTGGCAAACTgcgc                 >  2:314356/1‑251 (MQ=255)
                                                                                                                                                                                                                                              tttGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGtg    <  1:109623/250‑1 (MQ=255)
                                                                                                                                                                                                                                                tGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGtgct  <  1:22250/250‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                          
CGTGGATATCGATTCCATCCCCAGTTTTATTCAGGATGTGAATACTCATGGTCGCCTGATGCTGGCAAACGGTCAGCATGAAGTGGAGTTCCCGGTTGATCAGTGCATGAACTTCCACGCCGACAACCTTTCTCTGCATGAAAACGGTATGCGCATTACCGCGCTGGCGGGCGATAAAGTCGTTTACAGCCAGACTTACTACTCTATTGGCGGTGGCTTTATCGTTGATGAAGAGCATTTTGGCCAGCAGGATAGCGCACCGGTTGAAGTTCCTTATCCGTACAGTTCAGCAGCCGATCTGCAAAAACATTGTCAGGAAA‑CCGGGCTGTCACTCTC‑TGGCCTGATGATGAAAAACGAGCTGGCGCTGCACAGCAAAGAAGAGCTGGAACAGCACCTGGCGAACGTCTGGGAAGTCATGCGCGGCGGTATTGAGCGCGGTATTTCCACCGAAGGCGTGTTGCCTGGCAAACTGCGCGTTCCACGCCGTGCT  >  NC_000913/2929806‑2930295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑