Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	366,534	0	C	T	20.3%	160.5 / 10.2	67	R326K (AGA→AAA)	lacI	DNA‑binding transcriptional repressor LacI
Reads supporting (aligned to +/- strand):  ref base C (6/45);  new base T (13/0);  total (22/45)
Fisher's exact test for biased strand distribution p-value = 2.07e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCG  >  NC_000913/366287‑366773                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     aaTCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTttttcttt                                                                                                                                                                                                                                                   <  1:45120/251‑1 (MQ=255)  aTCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTttttctttt                                                                                                                                                                                                                                                  <  1:214941/251‑1 (MQ=255)           cattgtaaTAGCTGTTGCCTGTGTAAAATTGTTAACCGCTCACAATTCCACACAACATAAGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACTCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑gggg                                                                                                                                                                                                                                       <  2:84553/244‑4 (MQ=255)                    aGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑TGGGGCCCGGG‑GGGGTTTTTTTTTTACC‑A‑GTgaagatcggg                                                                                                                                                                                                                               >  1:124138/1‑242 (MQ=255)                    aGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTga                                                                                                                                                                                                                                       <  2:124138/242‑1 (MQ=255)                        gTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTCG‑TTTT‑GGGGGCCCGGG‑GGGGTTTTCTTTTTAcccgggggacggggcaccgc                                                                                                                                                                                                                            >  2:93651/1‑233 (MQ=255)                           tCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTg                                                                                                                                                                                                                       <  1:56889/250‑1 (MQ=255)                                 gtgAAATTGTTATCCGCTCACAATTCCACACAAAATACGAGCCGGAAGCAGAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGcc                                                                                                                                                                                                                 <  2:102181/250‑1 (MQ=255)                                    aaaTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTAT‑TAAT‑TGGCGCCCCGG‑GGGTTTTTCTTTTTACC‑C‑CTGAGACGGGACACCA‑CCGATTGCCCTTc                                                                                                                                                                                                             >  1:80729/1‑251 (MQ=255)                                     aaTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCa                                                                                                                                                                                                            <  1:315668/251‑1 (MQ=255)                                     aaTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCa                                                                                                                                                                                                            <  1:321053/251‑1 (MQ=255)                                     aaTTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGTG‑GAAT‑GGGCGCCAGGG‑GGGGTTTTTTTTTTACC‑A‑GTGGGACGGG‑CAAACGCTGATTGACCTTct                                                                                                                                                                                                            >  1:97353/1‑250 (MQ=255)                                            aTCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑Tg                                                                                                                                                                                                    <  1:251195/251‑1 (MQ=255)                                            aTCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑Tg                                                                                                                                                                                                    <  1:302573/251‑1 (MQ=255)                                             tCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TTTTTGGGGCCCCGGG‑GGGTTTTT‑TTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGAGTTCCCTTCACCGcccgg                                                                                                                                                                                                    >  2:136289/1‑248 (MQ=255)                                             cccGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑Tgg                                                                                                                                                                                                   <  2:69857/250‑1 (MQ=255)                                               cGCTCACAATTCCACACAACATGCGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCTCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCTGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGGGTGGGTTT‑CTTTGCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGcc                                                                                                                                                                                                 <  1:346363/251‑1 (MQ=255)                                                gCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TATTTGGGGGCCGGGG‑GGGTTTTT‑TTTTTACC‑A‑GTGAGACGGG‑CAACCGCTGATTTcccctccccccccggccc                                                                                                                                                                                                 >  1:300727/1‑236 (MQ=255)                                                           cacacaACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑GGGGTTTTTTTTTTACC‑C‑GTGCGGCGGG‑GAACAGCGGGTTGGCCTTCCCCGCCCTGGcccggacgggttg                                                                                                                                                                                      >  2:51174/1‑241 (MQ=255)                                                                 acaTACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTTCG‑GATT‑GGGCGCCCGGG‑GGGGTTTTTTTTTCACC‑A‑GTGAGAAGGG‑CAACAGCTGGATGCCCTTCACCGCC‑TGGCCCTGCGAGAGGTgcagca                                                                                                                                                                                >  1:269261/1‑250 (MQ=255)                                                                  caTACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑GTTT‑GGGCGCCCGGG‑GGGGGTTTCTTTTCTCC‑C‑GTGAGACGGG‑CAACCACTGGTTGCCCTTCACCCCC‑TGGCCCTggggggggttgacgaa                                                                                                                                                                               >  2:150120/1‑235 (MQ=255)                                                                      cGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCG‑TATTTGGGGGCCGGGG‑GGGTTTTT‑TTTTCACCCATGTG‑GAGGGG‑CA‑CAGCTGGTTTCCCTTCACCCCC‑CGGCCCTGggagaggttgagcaagaggg                                                                                                                                                                          >  1:276102/1‑237 (MQ=255)                                                                       gAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTc                                                                                                                                                                         <  1:215934/251‑1 (MQ=255)                                                                        aGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑GATT‑TGGGGCCCGGG‑GGGGTTTTTTTTTTACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTTACCCCC‑CGGGCCTGCGAGAGTTGCAGCAAGCgggcc                                                                                                                                                                        >  1:280260/1‑248 (MQ=255)                                                                              aaGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTg                                                                                                                                                                  <  1:91035/251‑1 (MQ=255)                                                                               aGCATAAAGTGTAAAGCCTGGGGTGCCAAATGCGTGAGCTACCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTTTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGTAGAGGCGGTTTGGG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTACCCT‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCCCGCttg                                                                                                                                                                 <  2:324328/251‑3 (MQ=255)                                                                                   tAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGGTTGCG‑TATT‑TGGGGCCCGGG‑GGGGTTTTTTTTTCAAC‑A‑GTGAGAAGGG‑CGACAGCTGATTGGCCTTCCCCCCC‑CGGCCCTGGGAGGGGTGCAGCAAACGGGCCACGCTGGTTTg                                                                                                                                                             >  1:124942/1‑251 (MQ=255)                                                                                    aaaGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATGGCGTTGCGCTCACTGCCCGCTTTCCAGGCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGAAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGc                                                                                                                                                            <  2:253296/251‑1 (MQ=255)                                                                                      aGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑TGGGGCCCGGG‑GGGGTTTTTTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑CGGCCCTGGGAGGGGTGGAGCAAGcagaacgggagagcacccgc                                                                                                                                                          >  1:31053/1‑232 (MQ=255)                                                                                      aGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGc                                                                                                                                                                             <  2:31053/232‑1 (MQ=255)                                                                                         gtAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCca                                                                                                                                                        <  1:113392/250‑1 (MQ=255)                                                                                             aGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAgg                                                                                                                                                   <  1:91775/251‑1 (MQ=255)                                                                                                           aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGtt                                                                                                                                     <  2:192292/250‑1 (MQ=255)                                                                                                           aaTGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGt                                                                                                                                      <  1:347019/249‑1 (MQ=255)                                                                                                                  gagCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGAtggtg                                                                                                                             <  1:142416/251‑1 (MQ=255)                                                                                                                   agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTGG‑TGTT‑GGGGGCCCGGG‑GGGTTTTTTTTTTTACC‑A‑GTGGGGCGGG‑CGACAGCTGATTGCCCTTCCCCGCC‑CGGCCCCGTGAGAGTTGGCGCAAACGGGCCCCGCCGGTTTGTCCCCGCAGGCG‑GCAATCCTGTTtggtggggg                                                                                                                            >  2:42669/1‑248 (MQ=255)                                                                                                                   agCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TATT‑GGGCGCCCGGG‑GGGGTTTTTTTTTTACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCCCC‑TGGCCCTGAGAGAGGTGCAGCAAGCGGTCCCCGCTGGGTTTCCCCCGCAGGCG‑GAAAACCTGTTTGAtggggg                                                                                                                            >  1:113097/1‑248 (MQ=255)                                                                                                                        aCTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAAc                                                                                                                       <  2:113097/251‑1 (MQ=255)                                                                                                                         cTCACATTAATTGCGTTGCGCTCACTGCTCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACa                                                                                                                                                                                                                           <  1:19531/152‑1 (MQ=255)                                                                                                                         cTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑TGGGGCCCGGG‑GGGGTTTTTTTTTCCCC‑C‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCCCCGCC‑CGGCCCTGGGAGGGGTGCAGCAAGCGGGCCCCGCTGGGTTGCCCCAGCAGGCG‑GAAATCCCGTTTGGTGGGGGGTAAcc                                                                                                                      >  1:154507/1‑250 (MQ=255)                                                                                                                              atgaaTTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑GGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCggg                                                                                                                 <  1:65018/248‑1 (MQ=255)                                                                                                                              aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGGTTTT‑GGCGGCCGGGG‑GGGTTTTTTTTTTC‑CC‑C‑GTGAGGCGGG‑GAACAACTGGTTGCCCTTCACCCCC‑CGGCCCCGAGAGAGTTGCGGCAAGCGGTCCCCGCCGGTTTGCCCCCGCCGGCGGAAAATC‑TGTTTGATGGGGGttgacaggggg                                                                                                                 >  2:235860/1‑242 (MQ=255)                                                                                                                              aTTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCggg                                                                                                                 <  1:73796/251‑1 (MQ=255)                                                                                                                                 aatttctgtgcgCTCACGGGCCGCTGTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑GATT‑GGGCTGCAGGT‑TGGTTGTTCTTTTCCCC‑A‑GTGAGTCGGG‑CAACAGCGGATTGCCCGTCACCGCC‑GGGCCCTGAGAGAGTTGCAGCAAGCGGTCGACGCTGGTTTACCCCAGCAGGCG‑AAAAGCCGGGTTGATGGTGGTTAACTGCGGGata                                                                                                              <  2:335448/243‑1 (MQ=255)                                                                                                                                     gcgttgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATaa                                                                                                           <  2:292662/250‑1 (MQ=255)                                                                                                                                         tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TATT‑TGGCGCCAGGG‑GGGGTTTTTTTTTTACC‑A‑GTGAGACGGG‑CAACAACTGATTGCCCTTCACCCCC‑CGGCCCCGAGAGAAGTGCAGCAAACGGGCCACCCCGGTTTGCCCCCGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACTGCggggcaaaaaaaa                                                                                                       >  1:355794/1‑240 (MQ=255)                                                                                                                                         tgcgCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCCGGG‑GGGTTTTTCTTTTTACC‑C‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCCCC‑TGGCCCTGAGAGAGGTGCAGCAAGCGGGCCCCGCTGGTTTGCCCCCGCCGGCG‑AAAATCCTGTTTGTTGGTGGGTAACGGCGGGGTATAACAtt                                                                                                       >  1:154656/1‑249 (MQ=255)                                                                                                                                                   gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTTCG‑TTTT‑TGGCGCCCGGG‑TGGTTTTTTTTTTTACC‑C‑ATTAGAAGGG‑CCACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGGGAGAGTTTCAGCAAGCGGTCCACGCTGGTTTGCCCCCCCAGGCG‑AAAATCCTGTTTTATGGTGGTTTACGGCgggggataaaaaaaactggttttg                                                                                            >  1:294170/1‑230 (MQ=255)                                                                                                                                                   gCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTc                                                                                             <  1:193439/250‑1 (MQ=255)                                                                                                                                                   gCCCGCTTGCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCtt                                                                                              <  2:59789/249‑1 (MQ=255)                                                                                                                                                               gTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTAt                                                                                <  1:38709/251‑1 (MQ=255)                                                                                                                                                                 cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGTGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATcc                                                                              <  1:225855/251‑1 (MQ=255)                                                                                                                                                                 cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGCGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATc                                                                               <  1:17438/250‑1 (MQ=255)                                                                                                                                                                 cGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATcc                                                                              <  1:93651/251‑1 (MQ=255)                                                                                                                                                                   ggAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCa                                                                            <  2:99252/251‑1 (MQ=255)                                                                                                                                                                                         aTTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAAc                                                      <  2:289752/251‑1 (MQ=255)                                                                                                                                                                                                   cGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCg                                            <  1:336312/251‑1 (MQ=255)                                                                                                                                                                                                       cAACGCTCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACt                                        <  1:200461/251‑1 (MQ=255)                                                                                                                                                                                                        aaCGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTc                                       <  1:269686/251‑1 (MQ=255)                                                                                                                                                                                                         aCGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCg                                      <  1:348381/251‑1 (MQ=255)                                                                                                                                                                                                         aCGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCAGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCg                                      <  2:246999/251‑1 (MQ=255)                                                                                                                                                                                                           gcgcgGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGt                                    <  2:342876/251‑1 (MQ=255)                                                                                                                                                                                                            cgcgGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTa                                   <  1:125533/251‑1 (MQ=255)                                                                                                                                                                                                                                g‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAg               <  1:42669/251‑1 (MQ=255)                                                                                                                                                                                                                                   aTT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCTCGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAgcg             <  1:232606/251‑1 (MQ=255)                                                                                                                                                                                                                                       gggCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGcc           <  2:154507/250‑1 (MQ=255)                                                                                                                                                                                                                                        ggCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCAt         >  1:149712/1‑251 (MQ=255)                                                                                                                                                                                                                                            ccAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAAAATGAGCTGTCTTTGGTATTGGCGTATCCCACCACCCAGATATTCGCACCAACGCGCAGCCCGGACCCGGGAATGGCGCGCCTTTCGCCCCGCGccctccg      >  2:72729/1‑245 (MQ=255)                                                                                                                                                                                                                                               ggg‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCg  <  1:125188/251‑1 (MQ=255)                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                     AATCCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCG‑TATT‑GGGCGCCAGGG‑TGGTTTTTCTTTTCACC‑A‑GTGAGACGGG‑CAACAGCTGATTGCCCTTCACCGCC‑TGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCG‑AAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCG  >  NC_000913/366287‑366773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑