Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,434,343 IS5 (+) +4 bp coding (61‑64/114 nt) ynaM ← protein YnaM

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1396044 =NA (NA)23 (0.660) 23/490 0.5 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 1434346 0 (0.000)coding (61/114 nt) ynaM protein YnaM
* ? NC_000913 = 1397238NA (NA)16 (0.460) 14/490 1.2 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 1434343 = 0 (0.000)coding (64/114 nt) ynaM protein YnaM

AGCAATGTTCTGGTATTGATAGGTCTTCCCTGTTTTGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTTATGGCGCAAGCTGAAGTGGCAACTGCAAACTATCTTATGC  >  NC_000913/1434103‑1434584
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
aGCAATGTTCTGGTATTGATAGGTCTTCCCTGTTTTGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggt                                                                                                                                                                                                                                         <  1:123665‑M2/251‑8 (MQ=255)
   gaaaaacgataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACt                                                                                                                                                                                                                                       <  1:55234‑M2/13‑1 (MQ=255)
           ataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttcccggctgattcgcaccttccTCAGAGCATGACAAATAATgca                                                                                                                                                                                                                              <  2:235544‑M2/22‑2 (MQ=255)
                  actggcgatgttattcacgctggccaacctgtttcgggcggcccaaatgatacgtccgtgggagagatctccctaaaaactggggataacgccttaaatggcgaagaaccggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATg                                                                                                                                                                                                                       >  2:24687‑M2/223‑251 (MQ=255)
                      gTCTTCCCTGTTTTGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgag                                                                                                                                                                                                                   >  1:222111‑M2/1‑222 (MQ=255)
                           cccTGTTTTGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatca                                                                                                                                                                                                               >  2:132225‑M2/1‑217 (MQ=255)
                             tattcacgctggccaacctgtttcgggagacccaaatgatacgtcagtgggagagctctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaactaggctgattcaaggcatttacgggagcaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCg                                                                                                                                                                                                             >  2:321569‑M2/212‑250 (MQ=255)
                                  ttGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgt                                                                                                                                                                                                        >  1:88152‑M2/1‑210 (MQ=255)
                                          caacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaatctggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaagtccgctcaaccatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCTTCACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTAtcatt                                                                                                                                                                                               <  2:245368‑M2/53‑3 (MQ=255)
                                               tgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgctcattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCa                                                                                                                                                                                                 <  2:116638‑M2/51‑1 (MQ=255)
                                               tgtttcgggcggaccaaatgatacggcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgctcattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCa                                                                                                                                                                                                 >  1:116638‑M2/194‑244 (MQ=255)
                                               tACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgcgatcatgtttgtcatctggagccat                                                                                                                                                                                           <  2:269761‑M2/250‑54 (MQ=255)
                                                    cgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTc                                                                                                                                                                                      <  1:88290‑M2/62‑1 (MQ=255)
                                                       ccccTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggg                                                                                                                                                                                  <  1:185960‑M2/251‑63 (MQ=255)
                                                        cccTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggt                                                                                                                                                                                 <  1:314964‑M2/251‑64 (MQ=255)
                                                         ccTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggtt                                                                                                                                                                                >  1:175634‑M2/1‑187 (MQ=255)
                                                                         cagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAAt                                                                                                                                                                 <  2:126379‑M2/83‑1 (MQ=255)
                                                                                     gcgcAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGTTATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcg                                                                                                                                                      >  2:4736‑M2/1‑159 (MQ=255)
                                                                                          gggAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgac                                                                                                                                                 <  1:231405‑M2/249‑96 (MQ=255)
                                                                                             aaCCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtg                                                                                                                                             <  1:24316‑M2/250‑100 (MQ=255)
                                                                                                      gataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAAttttt                                                                                                                                   >  1:173201‑M2/139‑251 (MQ=255)
                                                                                                         gtgtCTTTTGATTCCATGAACATATTCACTAACATACTATTGATTTTCATTGAGATATTGTATGTCTGATTCAGGATATGTTGACTTCTACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggcgccatagaccagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagta                                                                                                                                 <  2:359088‑M2/250‑112 (MQ=255)
                                                                                                           cgccttaaatggcgacgaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTCATCCt                                                                                                                              >  2:354129‑M2/134‑251 (MQ=255)
                                                                                                                                          atatTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc                                                                                               >  2:123665‑M2/1‑106 (MQ=255)
                                                                                                                                           tattatTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcc                                                                                              <  2:4950‑M2/251‑147 (MQ=255)
                                                                                                                                            attattGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtccc                                                                                             <  1:197061‑M2/251‑148 (MQ=255)
                                                                                                                                                  gATTTTCATTGCGATATTGTATGACTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttctggtcccgcatgg                                                                                       <  2:11947‑M2/251‑154 (MQ=255)
                                                                                                                                                      gcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGt                                                                                    >  1:124533‑M2/91‑250 (MQ=255)
                                                                                                                                                          ttGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagatt                                                                               <  2:336150‑M2/251‑162 (MQ=255)
                                                                                                                                                           tGCGATCTTGTATGTCTGGTTCAGGATATGTTGACTTATACCTCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattc                                                                              <  2:231547‑M2/251‑163 (MQ=255)
                                                                                                                                                                      aaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACa                                                                   <  1:210801‑M2/177‑1 (MQ=255)
                                                                                                                                                                       tGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaa                                                                   <  1:263486‑M2/250‑174 (MQ=255)
                                                                                                                                                                                                         ttttGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttacccca                                 <  2:193380‑M2/250‑208 (MQ=255)
                                                                                                                                                                                                                tGGGTTATTGGATATGCCAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggt                         <  2:305306‑M2/251‑216 (MQ=255)
                                                                                                                                                                                                                           ccccgcttattcgcaccttccTTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTTATGGCGCAAGCTGAAGTGGCAACTGCaa              <  1:64379‑M2/230‑1 (MQ=255)
                                                                                                                                                                                                                                 cAATCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttt        <  2:292891‑M2/251‑233 (MQ=255)
                                                                                                                                                                                                                                   aTCCCTAATTTTATTAGggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatc      >  2:103877‑M2/1‑17 (MQ=255)
                                                                                                                                                                                                                                       gcaccttccTTAGCGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTTATGGCGCAAGCTGAAGTGGCAACTGCAAACTATCTTATGc  >  1:257336‑M2/10‑251 (MQ=255)
                                                                                                                                                                                                                                         accttccTTAGAGCATGACTAAAAATGCTGAATATGATACGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTTATGGCGCAAGCTGAAGTGGCAACTGCAAACTATCTTATGc  >  1:29015‑M2/8‑249 (MQ=255)
                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 
AGCAATGTTCTGGTATTGATAGGTCTTCCCTGTTTTGCTACTTCCACTACAGCATCCCCTAGGATAATTCTTACTTCAGGAAGCTGCGCAGGGAACCACTTTAGGGTGTCTTTTGATTTCATGAAGATATTCCTTAAAATATTATTGATTTTCATTGCGATATTGTATGTCTGATTCAGGATATGTTGACTTATACATCGGTTTTGTCTGGGTTATTGGATATGCCAATCCCTAATTTTATTAGAGCATGACTAAAAATGCTGAATATGATAAGGAGCGAAGTGATTATCAGTATGCTGTTCATATAGCCTCGAATTAGTAATGTGTTATATATGATATAGTTGACAATTTTTATCCTGGGTGTTCTTAAAGTTCGTAGATAAACATTGTCGTTTCAGGTATACAGGAATGCTAACAGGTGGCGGCAAAAATCAGGCGGTTTATGGCGCAAGCTGAAGTGGCAACTGCAAACTATCTTATGC  >  NC_000913/1434103‑1434584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.