Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,069,379	IS1 (+) +9 bp	intergenic (‑103/+88)	yihQ ← / ← yihR	sulfoquinovosidase/putative sulfoquinovose mutarotase YihR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	21 (0.600)	21/490	0.6	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	4069379 =	0 (0.000)					intergenic (‑103/+96)	yihQ/yihR	sulfoquinovosidase/putative sulfoquinovose mutarotase YihR
*	?	NC_000913	3583428 =	NA (NA)	25 (0.720)	24/490	0.5	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 4069387	0 (0.000)					intergenic (‑111/+88)	yihQ/yihR	sulfoquinovosidase/putative sulfoquinovose mutarotase YihR

TGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/1978501‑1978751   |                                                                                                                                                                                                                                                         tGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGGGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGTCAGCCATCCGTCATCCATCTCACCACGTCAAAgggggg  >  2:320513/1‑248 (MQ=2)   |                                                                                                                                                                                                                                                         TGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/1978501‑1978751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.