Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,175,991	T→A	K111* (AAG→TAG)	gatZ ←	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,175,991	0	T	A	100.0%	175.6 / NA	53	K111* (AAG→TAG)	gatZ	putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (29/24);  total (29/24)

CAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATC  >  NC_000913/2175751‑2176222                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        cAACATGGGTGATGTGTACTGACTGAATCGCGCTGGCCTCACCGCCCAGAGCCGGAACTTCGGTGCCAATGAACTAGCTCAGTTGCCCACACTGGCAGTCTGTCGCCACAATTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATACCCCTCGCAGGACATTGACGCATCCAGATGAATTTTACT‑GGAGCCGGA‑CCGAACATCTTCCTATCCCAGCTcc                                                                                                                                                                                                                               >  1:551799/1‑250 (MQ=255)  aaCATGGGTGATGTGTACTGCCTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGAGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGATAACGGTATGGGATCCACCGCGCAGGACCTTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACTAAAATATTCCTATACCAGCTCg                                                                                                                                                                                                                               >  1:383912/1‑250 (MQ=255)                      actgaATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGccgca                                                                                                                                                                                                          <  1:201576/251‑1 (MQ=255)                       ctgaATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGccgcat                                                                                                                                                                                                         >  1:283460/1‑251 (MQ=255)                       ctgaATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGAAGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCTGCTCGACGGATTTTTCCATCGccgcat                                                                                                                                                                                                         >  2:393296/1‑251 (MQ=255)                          aaTGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGccgcatccg                                                                                                                                                                                                      <  1:531281/251‑1 (MQ=255)                             ggCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGccgcatccgcat                                                                                                                                                                                                   >  1:419207/1‑251 (MQ=255)                               cgcTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCAttt                                                                                                                                                                                                 <  2:52267/251‑1 (MQ=255)                                   ggCCGCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCtt                                                                                                                                                                                             <  2:418892/251‑1 (MQ=255)                                   ggCCCCACCGCCCGGAACCTGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCtt                                                                                                                                                                                             <  2:546202/251‑1 (MQ=255)                                                            tCGGGGCCAATGACATAGCTCAGTTGCTCACGCGGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTGTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCAGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAgat                                                                                                                                                                     <  2:17200/250‑1 (MQ=255)                                                                    aaTGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATcgccgc                                                                                                                                                            >  2:163066/1‑251 (MQ=255)                                                                      tGACGTAGCACAGTTGCCCACGCTGGCAATCTGTCGCCTCTCTTTCCGCAGCAAAGCAAAGCACAGCTGCTCGTTCCGCCACCGTTTCTGGTGCTAAAGGTGTGGGATCCCCCGCGCGGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCCGCAGTTTGGCCCAAGATGATCGccgccg                                                                                                                                                          >  2:236492/1‑251 (MQ=255)                                                                        aCATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACAGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCACCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGccgccg                                                                                                                                                          >  1:473023/1‑249 (MQ=255)                                                                            aGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaat                                                                                                                                                     >  2:88066/1‑250 (MQ=255)                                                                              cTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaataat                                                                                                                                                  >  2:240949/1‑251 (MQ=255)                                                                                cAGTTGCTCCCGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGGTTCTGGTGCTAACGTTATGGGATCCCACGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaataat                                                                                                                                                  <  2:517152/249‑1 (MQ=255)                                                                                cAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCACAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACCTTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGaataat                                                                                                                                                  >  1:517152/1‑249 (MQ=255)                                                                                cAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGc                                                                                                                                                <  1:252008/251‑1 (MQ=255)                                                                                    tGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTc                                                                                                                                            >  1:556918/1‑251 (MQ=255)                                                                                     gCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTcg                                                                                                                                           >  1:26984/1‑251 (MQ=255)                                                                                        cACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCATAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGGTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTcgcg                                                                                                                                         <  2:293893/250‑1 (MQ=255)                                                                                            cTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCa                                                                                                                                     >  1:395573/1‑250 (MQ=255)                                                                                                  aTCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGCTCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCa                                                                                                                               >  1:540997/1‑250 (MQ=255)                                                                                                      gTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACtt                                                                                                                           <  1:358652/250‑1 (MQ=255)                                                                                                          ccACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCg                                                                                                                      >  2:359205/1‑251 (MQ=255)                                                                                                            acacTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGc                                                                                                                    >  1:145208/1‑251 (MQ=255)                                                                                                              acTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCa                                                                                                                   >  1:443809/1‑250 (MQ=255)                                                                                                               cTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAAt                                                                                                                 >  1:512795/1‑251 (MQ=255)                                                                                                                   ccGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCAGCAAGATGAATTTTTCT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCAGCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGt                                                                                                              <  2:368470/250‑1 (MQ=255)                                                                                                                         caaagcaaagCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACa                                                                                                       <  1:239732/251‑1 (MQ=255)                                                                                                                               aaagcaCAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTcg                                                                                                 >  2:412127/1‑251 (MQ=255)                                                                                                                                          gcTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGc                                                                                      >  1:313960/1‑251 (MQ=255)                                                                                                                                                 ccGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCAGCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCGCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGgtgt                                                                                <  2:45694/250‑1 (MQ=255)                                                                                                                                                         gTTTCTGGTGCTACCGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTCAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGt                                                                       >  1:446575/1‑251 (MQ=255)                                                                                                                                                           ttCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGta                                                                      <  2:556918/250‑1 (MQ=255)                                                                                                                                                            tCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGtat                                                                     <  2:288901/250‑1 (MQ=255)                                                                                                                                                                gTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCAAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCg                                                                >  2:340966/1‑251 (MQ=255)                                                                                                                                                                              ggATCCCCCGCGCAGGACATTGACCCATCAAGATGCATTTTCCT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTAGCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTTTCATTCCGGTATAACCGCCAAATTGATTGAc                                                  <  2:110296/251‑1 (MQ=255)                                                                                                                                                                                  cccccGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACCTATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGCGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTgg                                              >  1:530234/1‑251 (MQ=255)                                                                                                                                                                                     ccGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGttt                                           >  2:2145/1‑251 (MQ=255)                                                                                                                                                                                     ccGCCCAGGACTTTGACGCCTCAAGAGAAATTTTCCT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGTATTTTTCCATCGCCGCACCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGGGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGttt                                           <  2:192127/251‑1 (MQ=255)                                                                                                                                                                                      cgcgCAGGAAATTTACGCACCAAGATGCATTTTACT‑GAAGCCGGCCAC‑AACATATTCCTATACCAGCCCGCCGGATTTTTCCACCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTTCCGGTGTCATTCCGGTATACCCGCCAAATTGATTGACCTGGTTTg                                          <  2:384249/251‑1 (MQ=255)                                                                                                                                                                                         gcAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACg                                       <  1:412127/251‑1 (MQ=255)                                                                                                                                                                                          cAGGACATTGACGCAGAAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCGCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAACCCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGt                                      <  2:283460/251‑1 (MQ=255)                                                                                                                                                                                           aGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGACCATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAACTTGATTGACCGGGTTTGCCGtt                                     >  2:464924/1‑251 (MQ=255)                                                                                                                                                                                           aGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTAGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGACAGTCTGCCGGGGTCATTCCGTTATAACCACAAAATTGATTTACCTGGTTTgcggtt                                     >  2:401745/1‑246 (MQ=255)                                                                                                                                                                                            ggACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTCCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCACCTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTg                                    <  2:89366/251‑1 (MQ=255)                                                                                                                                                                                                aTTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTc                                <  1:240949/251‑1 (MQ=255)                                                                                                                                                                                                                  aatttACTTGAAGCCGAC‑ACAAACATATCCCCATACCAGCCCGTGGGATTTGTCCACCGCCGCACCCGCATTTTTGTGCTTCCAGCAGTTTGGCCCCATATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAATCCCAACTTTATCGGCAATCGGAAAAACAAATTCGCAAAAGTCTGCCGGTGTCATTCGGGTATAACCGCCAAATTGAGTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGcgcg               <  2:325701/249‑1 (MQ=255)                                                                                                                                                                                                                     ttACT‑GAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCt           >  1:411770/1‑251 (MQ=255)                                                                                                                                                                                                                           gAAGCCGGC‑ACGAACATATTCCTATACCAGCTCGACGGATTTTTCAATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAAACCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACATGGTTTGACGTTGCTTCAATCAGCCCTTTGCGCGCGCTGTTGc      >  1:44832/1‑251 (MQ=255)                                                                                                                                                                                                                               ccgcc‑ACGAACATATTCCTAGCCCAGCTACACGGATTTTCCCGTCCCCGCATCCGCATTTGCTTGCTGCCAGCAGTTGGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGGGTGCAAACCCAACTTTATCGGCAATCTTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATc  <  2:395573/247‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        CAACATGGGTGATGTGTACTGACTGAATGGCGCTGGCCTCACCGCCCGGAACCGGAACTTCGGTGCCAATGACATAGCTCAGTTGCTCACGCTGGCAATCTGTCGCCACACTTTCCGCAGCAAAGCAAAGCACAGCAGCTCGTTCCGCAACCGTTTCTGGTGCTAACGGTATGGGATCCCCCGCGCAGGACATTGACGCATCAAGATGAATTTTACT‑GAAGCCGGC‑ACGAACATATTCCTTTACCAGCTCGACGGATTTTTCCATCGCCGCATCCGCATTTTCTTGCTGCCAGCAGTTTGGCCCCAGATGATCGCCGCCGAGAATAATGCGTTCGCGTGCAAACCCAACTTTATCGGCAATCGTAAAAACAAATTCGCGAAAGTCTGCCGGTGTCATTCCGGTATAACCGCCAAATTGATTGACCTGGTTTGACGTTGCTTCAATCAGCACTTTGCGCGTGCTGTTGCGATC  >  NC_000913/2175751‑2176222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑