Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,276	A→C	S111A (TCT→GCT)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,276	0	A	C	100.0%	188.1 / NA	50	S111A (TCT→GCT)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (28/22);  total (28/22)

CAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGAT  >  NC_000913/2003042‑2003522                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       cAGTTACTGGAGCACGAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCa                                                                                                                                                                                                                                        <  1:307036/251‑1 (MQ=255)    ttACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGTTAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCCCGTCCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCaga                                                                                                                                                                                                                                      <  1:553219/250‑1 (MQ=255)         ggAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAACCCATCAAGGCCAAGAGTTTTAGCATCAATCGGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTa                                                                                                                                                                                                                                  <  2:310023/249‑1 (MQ=255)                  gTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGt                                                                                                                                                                                                                       >  1:361995/1‑251 (MQ=255)                              tCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGt                                                                                                                                                                                                           >  1:341477/1‑251 (MQ=255)                               cGTTATTTTTAACGCTAAACCCAGCAAGGCCAAGTGTTTTAGCATCAATCTGCTGCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTc                                                                                                                                                                                                          <  2:409684/251‑1 (MQ=255)                               cGTTATTTTTAACGCTAAAACCAGCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGGGATAGTCTGGTTATCAGTTGCGCCAACCTGGATTTTCAGGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGt                                                                                                                                                                                                           <  1:378920/250‑1 (MQ=255)                               cGTTATTGTTAACGCTAAAACTATCAAGGCCAAGAGGTTTAGCATCAAGCTGCGTCAGATCGATAGTGATAGTCTGGTTATCATTGGCGCCAACCTGGAGTTTAATGGAGCCATTTTTTGCCAGCACGTGCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACGCAGAGTTAGTACCGGTAGTGGCCTGTACCGt                                                                                                                                                                                                           <  2:507401/250‑1 (MQ=255)                                               aaaaCCATCAAGGCCAAGAGTTTTAGCATCAATCTTCTTCAGATCGATAGTGATAGTCTGGTTAGCATTTGCGCCAACCTGGAGTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACg                                                                                                                                                                                            <  2:119154/249‑1 (MQ=255)                                                  aCCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACCCTGt                                                                                                                                                                                        >  2:189056/1‑250 (MQ=255)                                                  aCCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACAGTCAGTTCACGCACACGCTGt                                                                                                                                                                                        >  2:512464/1‑250 (MQ=255)                                                     aTCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGCTTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCCGTTCACGCACACGCTGTAAgt                                                                                                                                                                                    >  1:211779/1‑251 (MQ=255)                                                     aTCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgt                                                                                                                                                                                    >  1:101763/1‑251 (MQ=255)                                                      tCAAGACCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgtt                                                                                                                                                                                   >  2:293353/1‑251 (MQ=255)                                                        aaGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgttgt                                                                                                                                                                                 >  1:7764/1‑251 (MQ=255)                                                         aGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAgttgtt                                                                                                                                                                                >  2:243906/1‑251 (MQ=255)                                                              aaGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGttgttgttg                                                                                                                                                                            >  2:543977/1‑250 (MQ=255)                                                                  gTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTc                                                                                                                                                                       <  1:543977/251‑1 (MQ=255)                                                                    ttgaGCATCAATCTGCTTCATATCGATAGTGATAGTCTGGTTAGCATTTGCGCCACCCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACAAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCg                                                                                                                                                                      <  1:86603/247‑1 (MQ=255)                                                                          aTCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAgcg                                                                                                                                                               >  1:358329/1‑251 (MQ=255)                                                                                  gCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCg                                                                                                                                                       >  1:546778/1‑251 (MQ=255)                                                                                     tCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGGTAGCAGACAGATCAGACTCTGAGTTAGTACCGGTAGTGGCATGTACCGTCAGTTAACGCACACGCTGTAAGTTGTTGTTGATTGCGGACAGCGCGCCTTCggtg                                                                                                                                                    >  1:532119/1‑251 (MQ=255)                                                                                       aGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCggtggt                                                                                                                                                  >  1:538151/1‑251 (MQ=255)                                                                                             atagtgatagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCTGTCAGCGCGCCTTCGGTGGTCTgcgc                                                                                                                                            >  2:135456/1‑251 (MQ=255)                                                                                             atagtgatagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTgcgc                                                                                                                                            >  1:54883/1‑251 (MQ=255)                                                                                                gtgatagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGGTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAAc                                                                                                                                         <  1:415219/251‑1 (MQ=255)                                                                                                  gatagtCTTGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTGGCCAGCACGTTGATGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACgg                                                                                                                                       <  1:67646/251‑1 (MQ=255)                                                                                                   atagtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGga                                                                                                                                      <  1:168219/251‑1 (MQ=255)                                                                                                     agtCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGgaga                                                                                                                                    <  2:421672/251‑1 (MQ=255)                                                                                                             tATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGAAACGGAGATACcgtcg                                                                                                                            >  1:83581/1‑251 (MQ=255)                                                                                                                cATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTg                                                                                                                         >  1:389320/1‑251 (MQ=255)                                                                                                                     gcgcCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACGGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTGCCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGt                                                                                                                     <  2:319673/250‑1 (MQ=255)                                                                                                                                  ttttCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTAAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGAACACGCTGTTAGTTGTTGTTGATTTCGGCCAGCGCGCCTTCGGTGGTCTGCGCAACGGGAAAACCGTTGGTGGCGGTAAGGGCCGCCTgg                                                                                                       >  2:515775/1‑250 (MQ=255)                                                                                                                                            gCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCtt                                                                                             <  2:386755/251‑1 (MQ=255)                                                                                                                                                          gCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAgggg                                                                               >  2:203293/1‑249 (MQ=255)                                                                                                                                                          gCACGTTCACGCCGTTGAACTGGGTCTGAACAGATACGCGGTCAATTTCATCCAGACGTGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTg                                                                               <  2:28037/251‑1 (MQ=255)                                                                                                                                                                   cGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACCCGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCACCGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTCATGTTAGCGGTGAAACGGttg                                                                      >  2:524628/1‑250 (MQ=255)                                                                                                                                                                    gCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGGAAGTTGTTGTTGATTTCGGACAGCCCGCCTTCGGTGGTCTGCGCCACGGAGGTACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGGGAAACGGtt                                                                       >  2:422888/1‑249 (MQ=255)                                                                                                                                                                       ggtGAACTGGGTATGACCAGATACGCGGTCAATTTCACCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTGCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGCGAAACGGTTAGCaa                                                                  <  2:237081/249‑1 (MQ=255)                                                                                                                                                                         tGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTGCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGGCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAAt                                                                 <  2:2000/250‑1 (MQ=255)                                                                                                                                                                           aaCTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCga                                                              <  1:161831/251‑2 (MQ=255)                                                                                                                                                                                     gACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGACTGACCCGcc                                                    >  2:152743/1‑250 (MQ=255)                                                                                                                                                                                       ccAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGCATCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTGACGGGCCGCCTGAGTCAGGCCTGTAATGTTAGAGGTGAAACGGTTAGCAATCGACTGACCCGCTGc                                                  <  2:211779/251‑1 (MQ=255)                                                                                                                                                                                                             ccAGACGGGATTTAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGCACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACgg                                                                         >  1:490666/1‑206 (MQ=255)                                                                                                                                                                                                             ccAGACGGGATTTAAGTTCGGCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGCACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACgg                                                                         <  2:490666/206‑1 (MQ=255)                                                                                                                                                                                                               aGACGGGATTTAATTTCGTCCTGGCTAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTAACGTCAGTTCACGCCCACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGACTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACg                          >  2:346374/1‑251 (MQ=255)                                                                                                                                                                                                                       tttAATTTCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAACCGGTTAGCAATCGACTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGcc                    >  2:548506/1‑249 (MQ=255)                                                                                                                                                                                                                             ttCGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGGGGGGAAACGGGTAGCAATCGACTGACCCGCTGCGTCCTCCTTCGCGCTGTTAATACGCAAGCCagaagaa             >  2:56598/1‑249 (MQ=255)                                                                                                                                                                                                                               cGTCCTGGATAGCAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGACTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAagacag           <  1:243906/250‑1 (MQ=255)                                                                                                                                                                                                                                       aTAGCAGACAGATCAGCCTCAGAGTTAGTACAGGTAGTAGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGCCAACGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTACTGGTAGAGGTGAAACGGTTAGCAATCGACTGACCCGCTGCGTCATCCTTCGCGCTGTTAATCCGCAAGCCAGAAGACAGACGCTCGAt  >  1:509141/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       CAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTTAATACGCAAGCCAGAAGACAGACGCTCGAT  >  NC_000913/2003042‑2003522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑