Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,598,172	T→G	intergenic (‑5730/+445)	hipB ← / ← lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,598,172	0	T	G	100.0%	69.2 / NA	21	intergenic (‑5730/+445)	hipB/lsrK	antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/7);  total (14/7)

CCAGCAAAACACCCAGCGTCAATCTACTGAATTTCGTTGTCAGTCCAGAGGATTTACGCAAATTAACCGTCGATGTTTTACCTGCCCTGCGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTG  >  NC_000913/1597927‑1598413                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ccAGCAAAACACCCATCTTCAATCTACTTAATTTCGTTGTCATTCCAGAGGATTTACGCAAATTATCCGGCGATGTTTTACCTGCCCTGCGAGTTAATTCCGGGCAGGCCTTAAATCCCTGTAGAGTGCAATTCCATATCACGCGATAGAATCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGt                                                                                                                                                                                                                                               <  2:426869/250‑1 (MQ=255)                          aCTGAATTTCGTTGTCAGTCCAGAGGATTTACGCAAATTAACCGTCGATGTTTTACCTGCCCTGCGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGa                                                                                                                                                                                                                       >  1:537409/1‑249 (MQ=255)                            tGAATTTAATTGTCAGTCCAGAGGATTTACGCAAATTAACCGTCGATGTTTTACCTGCCCTGCGAGTTAATTCCGAGCAGACCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATCGATTCTCTTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGGGTGTTAGATGGAGGAGGGAAggg                                                                                                                                                                                                                   >  2:333369/1‑250 (MQ=255)                                                gagGATTTACGCAAATTAACCGTCGATGTTTTACCTGCCCTGCGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCt                                                                                                                                                                                               >  1:283813/1‑251 (MQ=255)                                                                       cGATGTTTTTCCAGCCCTGCGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGAtt                                                                                                                                                                         <  1:172214/250‑1 (MQ=255)                                                                                     ccctgcGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATGTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGtt                                                                                                                                                          <  2:26035/251‑1 (MQ=255)                                                                                                             ccTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGAGGGAAGGTGTCACTTTGGCTAATTTGCTAATGATTTCGAATTGCTAGCTTAAAAATTGCATAGTTCTTCTTTTATTTAAATATGAACt                                                                                                                                   >  2:527644/1‑250 (MQ=255)                                                                                                                                      ccATATCACGCGATAGATTCTAGTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTTTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGaa                                                                                                          <  1:568354/250‑1 (MQ=255)                                                                                                                                                   tAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAAt                                                                                                                                                                                  >  2:431610/1‑165 (MQ=255)                                                                                                                                                   tAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAAt                                                                                                                                                                                  <  1:431610/165‑1 (MQ=255)                                                                                                                                                                       gCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTatcatcatc                                                                        >  2:155142/1‑251 (MQ=255)                                                                                                                                                                            agataCAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGAGAGTTGGCGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTAAGAAATGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGATTTATTGTTGCTTAATAAGAAAACTGTTTCTTTAAATAGGAAAATTATCTTCATCATAtt                                                                   >  2:333823/3‑251 (MQ=255)                                                                                                                                                                                              cACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACt                                                  >  2:310939/1‑250 (MQ=255)                                                                                                                                                                                                                  aGACATTAAAAATGAAACTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGttt                             >  2:372572/1‑251 (MQ=255)                                                                                                                                                                                                                        tAAAAATGAAACTTAATAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTCTCAATAGGAAAAGTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTaa                        <  2:193030/250‑1 (MQ=255)                                                                                                                                                                                                                                aaaCTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGaaaa                                                                                   >  2:2384/1‑183 (MQ=255)                                                                                                                                                                                                                                aaaCTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGaaaa                                                                                   <  1:2384/183‑1 (MQ=255)                                                                                                                                                                                                                                 aaCTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTg              >  1:390444/1‑251 (MQ=255)                                                                                                                                                                                                                                  aCTTATTAAATTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGc             >  1:243379/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCAtt      >  1:567157/1‑249 (MQ=255)                                                                                                                                                                                                                                             tGTCAGAGGGCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAACAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAgtg  >  1:449582/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  CCAGCAAAACACCCAGCGTCAATCTACTGAATTTCGTTGTCAGTCCAGAGGATTTACGCAAATTAACCGTCGATGTTTTACCTGCCCTGCGAGTTAATTCCGAGCAGGCCTGAAATACCTGTAGAGTGCAATTCCATATCACGCGATAGATTCTATTCATATAAACGCTCCATATACAAACAATACAGCCACGAAAGAGATGAGTCGTCAGACATTAAAAATGAAACTTATTAAATTGTCAGAGGTCTGTATTGAGTGTTAGTTGGAGGTGGGAAGGTGTCAATTTGGCTAATTTGCTAATGCTTTCGAATTGATAGATTAAAAATTGCATAGTTCTTATTTTATTTAAATATGAACTATGAGTTATTGTTGCTTAATAAGAAAACTGTTTCTTTCAATAGGAAAATTATCATCATCATATTTAACAAAGAATAGCACTAATTGCTAAAAATCGAAGTTTATTAAACCCCTTTTGCATCCATTAGTG  >  NC_000913/1597927‑1598413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑