Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,123,556	C→T	E167K (GAA→AAA)	grxB ←	reduced glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,123,556	0	C	T	97.1%	115.9 / ‑3.8	35	E167K (GAA→AAA)	grxB	reduced glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (14/20);  total (14/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.77e-01

GCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGC  >  NC_000913/1123317‑1123802                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gcagcaTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACgg                                                                                                                                                                                                                                             <  2:416507/251‑1 (MQ=255)  cagcaTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGc                                                                                                                                                                                                                                            <  1:93487/251‑1 (MQ=255)  cagcaTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGc                                                                                                                                                                                                                                            <  1:57776/251‑1 (MQ=255)      aTGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGGCAGGATTAAATCGCCATTGATGATAACAAATTGGTTTGTGTCTGTTTCGCCATATTATCGCGGGAATCAGCAACGCGGCTTTGCCAGTTAATTCCGGCTACCAGCGGCAGATTACGCAGGAGCGGGAATAGATGAATATCATCTTCCGAAAGTTTGCCATTCACGGCggtt                                                                                                                                                                                                                                        >  1:254676/1‑248 (MQ=255)                 gCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCTCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGAt                                                                                                                                                                                                                             <  2:279507/251‑1 (MQ=255)                    aTAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCCTTGATGATAACAAATTGATTTGTGTCTGTTTCGCCCTATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTTCGGCTACCAGCGTCAGATTACGCAGTAGCGGGGATAGCTGAATATCATCTTCCGAAAGTTTGGCATTCACCGCGTTCGGTTTTACGATCa                                                                                                                                                                                                                           >  1:120883/1‑250 (MQ=255)                                      aaaaCTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGGCAGGATTAAATCGCCATTTATGATAACAAATTTATTTTTGTCTTTTTCGCCATATTTTCCCGGTCATCATCAACCCGGCTTTGCCAGTTTATTTCCGCTACCAACGTCCGGTTTCCCCGTAGCGGGAATAGCTTAATATCATCTTCCGAAAGTTTTCCCTTCCCCGCGTTCGGTTTTACGATCAGttttttccgcgcccgta                                                                                                                                                                                                         >  1:159297/1‑236 (MQ=255)                                       aaaCGGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAAGCGCCATTGATGATAACAAATTGATTTGTGTCTGGTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCTCGTaaa                                                                                                                                                                                                       <  2:290163/251‑1 (MQ=255)                                         aCTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTTAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAatc                                                                                                                                                                                                     <  1:47397/251‑1 (MQ=255)                                             aaaaCGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAAGCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCg                                                                                                                                                                                                 <  1:436923/251‑1 (MQ=255)                                                        aaCCCGCTAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCAGTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCtt                                                                                                                                                                                      <  1:319002/251‑1 (MQ=255)                                                              ggAATTCAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAg                                                                                                                                                                                <  2:558699/250‑1 (MQ=255)                                                               gAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGa                                                                                                                                                                               <  2:49158/251‑1 (MQ=255)                                                                 aTACAGGCTGCCGCGGCGGGTTAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGAcc                                                                                                                                                                             <  2:290202/251‑1 (MQ=255)                                                                                                     aTTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCCGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGATTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATAATCGCTGATATTCTTCATCCGACCGTCAGAGTGGGCCAGCAGGTCGGAAAAATTACCcgc                                                                                                                                         >  2:364716/1‑251 (MQ=255)                                                                                                             tAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCct                                                                                                                                 >  1:2477/1‑251 (MQ=255)                                                                                                              aaCAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCCCGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCCCGTAAATCATCGCTGCTATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTCCCCTCGCTGGCctc                                                                                                                                >  2:426495/1‑251 (MQ=255)                                                                                                                  aaTTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCcgcagataggaagagg                                                                                                                            >  2:2572/1‑238 (MQ=255)                                                                                                                  aaTTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCcgc                                                                                                                                         <  1:2572/238‑1 (MQ=255)                                                                                                                     tGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCtctttctt                                                                                                                          <  1:443678/250‑1 (MQ=255)                                                                                                                               cTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTACGGCTACCAACGTCAGATTACGCAGTAGCGGGAATAGCTCAATATCATATTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCCCGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCCGGTCGGCAAAATTACACGCGCTGGCCTCTTTCTTGTcgacggaa                                                                                                                >  2:276274/1‑247 (MQ=255)                                                                                                                                      gCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTgcg                                                                                                        >  2:215230/1‑251 (MQ=255)                                                                                                                                                      aaTCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGa                                                                                         >  1:420415/1‑250 (MQ=255)                                                                                                                                                          aGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTa                                                                                           <  2:124085/244‑1 (MQ=255)                                                                                                                                                          aGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTa                                                                                           >  1:124085/1‑244 (MQ=255)                                                                                                                                                             aaCGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACtcat                                                                                 <  1:84981/251‑1 (MQ=255)                                                                                                                                                                        gCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATAGTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGAt                                                                      <  1:24138/251‑1 (MQ=255)                                                                                                                                                                          cAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGAttt                                                                    <  1:311690/251‑1 (MQ=255)                                                                                                                                                                              tAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTGCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGc                                                                 <  1:462208/250‑1 (MQ=255)                                                                                                                                                                                          ccAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAAc                                                    <  2:254676/251‑1 (MQ=255)                                                                                                                                                                                                       aCGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTttgttg                                        <  2:161055/250‑1 (MQ=255)                                                                                                                                                                                                                 gggAATAGCTGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCAtt                             <  1:426495/251‑1 (MQ=255)                                                                                                                                                                                                                          tGAATATCATCTTCCGAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTgcg                    >  1:114185/1‑251 (MQ=255)                                                                                                                                                                                                                                         gAAAGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAAAAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAAt     >  2:498550/1‑251 (MQ=255)                                                                                                                                                                                                                                            aGTTTGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGc  >  2:284446/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       GCAGCATGTGTCACCAGCGATAAACTCGCCAGCAGAAAAAACTGAAAACGGCGGCAACCCGCGAATACAGGCTGCCGCGGCGGGTCAGGATTAAATCGCCATTGATGATAACAAATTGATTTGTGTCTGTTTCGCCATATTATCGCGGTAATCAGCAACGCGGCTTGGCCAGTTAATTCCGGCTACCAGCGTCAGATTACGCAGTAGCGGGAATAGCTGAATATCATCTTCCGAAAGTTCGCCATTCACGGCGTTCGGTTTGACGATCAGTTTGTCCAGCGCACGTAAATCATCGCTGATATTCTTAATCAGACCGTCAGAGTGGGCCAGCAGGTCGGCAAAATTACCCGCGCTGGCCTCTTTCTTGTCGACGAAATATTTGCGCGCGGCGGGAGTAGAAAACTCATCAAATGCCGATTTGGCAAAACGCGGCAACAGCAGTTTGTTGGCGTAGCCATTGACCTTGCGCAGCCACTCTTCAATGGC  >  NC_000913/1123317‑1123802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑