Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	917,966	G→A	M498I (ATG→ATA)	ybjD →	DUF2813 domain‑containing protein YbjD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	917,966	0	G	A	100.0%	246.8 / NA	67	M498I (ATG→ATA)	ybjD	DUF2813 domain‑containing protein YbjD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/32);  total (35/32)

AAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTCAGCCCGTTTT  >  NC_000913/917729‑918206                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 aaaGACACTGGTTAAATTTGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATCTGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGCTATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGAGTTCCACCGCATGGCGCCAATCACGAAACATGTACCGATAAATCTACGccaa                                                                                                                                                                                                                                      >  2:333119/1‑247 (MQ=255)    gCCACTGGTTAAATTGGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTa                                                                                                                                                                                                                                  <  1:192293/251‑1 (MQ=255)          ggTTAAATTTGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCg                                                                                                                                                                                                                             >  1:70163/1‑250 (MQ=255)             tAAATTTTCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGaaa                                                                                                                                                                                                                          <  2:506557/250‑1 (MQ=255)             aaaaTTTGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAg                                                                                                                                                                                                                         <  2:514299/250‑1 (MQ=255)                  tggCCCGCCGAAGGGGGATTGAATGGCATGTACTGGGCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATGTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGAt                                                                                                                                                                                                                     <  2:93776/248‑1 (MQ=255)                   tGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATc                                                                                                                                                                                                                    <  1:486452/250‑1 (MQ=255)                    gCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTCCCGATAAATCTACGCAAAATTATCTCGAAAGCGATcc                                                                                                                                                                                                                   <  1:200912/250‑1 (MQ=255)                    gCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATcc                                                                                                                                                                                                                   >  1:381705/1‑250 (MQ=255)                                         tGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATc                                                                                                                                                                                                                                              <  1:257486/202‑1 (MQ=255)                                         tGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATc                                                                                                                                                                                                                                              >  2:257486/1‑202 (MQ=255)                                             aggtaccggccGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCTCCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGcc                                                                                                                                                                                         <  1:534576/241‑1 (MQ=255)                                              tGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGcc                                                                                                                                                                                         >  1:368843/1‑250 (MQ=255)                                                    ggTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAAACCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGCAAGCGATCCATAGCTCTTCCAAACCCGATCTTGCCATTGa                                                                                                                                                                                    >  2:380315/1‑249 (MQ=255)                                                              gATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGCTAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATgg                                                                                                                                                                         >  2:315943/1‑250 (MQ=255)                                                                  aaGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGACGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGCTAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGcc                                                                                                                                                                    >  1:239014/1‑250 (MQ=255)                                                                        ggAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGAcgt                                                                                                                                                              <  1:72336/251‑1 (MQ=255)                                                                          aagaaATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGAcgtcg                                                                                                                                                            >  1:52973/1‑251 (MQ=255)                                                                              aaTATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGTACGTCgtggt                                                                                                                                                        <  2:577762/251‑1 (MQ=255)                                                                                tatGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATTTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGgtgt                                                                                                                                                      >  1:238269/1‑251 (MQ=255)                                                                                            gTACGCAGCCTGTTGAATAACGATCGGTAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTAcc                                                                                                                                          <  2:400540/251‑1 (MQ=255)                                                                                                 cAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCaa                                                                                                                                                                                                      >  2:216170/1‑186 (MQ=255)                                                                                                 cAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCaa                                                                                                                                                                                                      <  1:216170/186‑1 (MQ=255)                                                                                                  aGCCTGGTGAAGAACGATCGGGAAGCCGAACGAGAACATGTAACGGCGTTTCCGGCGCTGGATATGGAACATTTTATGTAGCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGGCCCGAGAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCGCGTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTCCCGACgcg                                                                                                                                    <  2:85139/251‑2 (MQ=255)                                                                                                    ccTGTTGAATAACGATCGGGAAGCCGAACGAGAACCTTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACgcggc                                                                                                                                  >  2:571140/1‑248 (MQ=255)                                                                                                         tGAAGAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGAGATGGATATGGAACAGTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAAGACCGGAAAATGTACCGATAAATCTTCGCAAAATTATCTCGAAAGCGAACCAGCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGaa                                                                                                                              <  2:151363/250‑1 (MQ=255)                                                                                                               aCGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATg                                                                                                                       >  1:143936/1‑251 (MQ=255)                                                                                                                 gATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGATGCTGAAAAAAATGtt                                                                                                                     >  2:577213/1‑251 (MQ=255)                                                                                                                  aTCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCCTTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGttt                                                                                                                    >  2:543949/1‑250 (MQ=255)                                                                                                                     gggAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTtctc                                                                                                                  >  2:550355/1‑250 (MQ=255)                                                                                                                     gggAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTtctc                                                                                                                  >  1:418477/1‑250 (MQ=255)                                                                                                                      ggAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCAc                                                                                                                >  1:482985/1‑251 (MQ=255)                                                                                                                         aGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCAcgcg                                                                                                             >  1:271514/1‑251 (MQ=255)                                                                                                                                 gagaACATTTAACGGCGTTACCGGCGCTGGAGATGGAACAGTTTATGGATCGCCAGGGATTTTCCGGTGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGACCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGTACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGGTCTGtgg                                                                                                     <  2:498696/251‑1 (MQ=255)                                                                                                                                  agaACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGtggc                                                                                                    >  1:177154/1‑251 (MQ=255)                                                                                                                                    aaCATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCCCCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTACCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAACAAAATGTTCTCACGCGTGCTGtggctg                                                                                                  >  1:50304/1‑251 (MQ=255)                                                                                                                                      cATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCAGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGCCGCTGCTGAAAAAAATGTTCTCACGCGTGCTGtggctggg                                                                                                >  2:220582/1‑250 (MQ=255)                                                                                                                                       aTTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTTGTCCACCTCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGgcg                                                                                               <  1:360552/251‑1 (MQ=255)                                                                                                                                                 gTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCgggcccg                                                                                     >  2:173149/1‑246 (MQ=255)                                                                                                                                                                tatGGAACATTTTATGTATCGCCAGGGCTTTTCCGATGTGTTCCACCGCATGGCGCAGATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACcgcgc                                                                       >  1:113861/1‑249 (MQ=255)                                                                                                                                                                 atGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTc                                                                                        <  1:433826/232‑1 (MQ=255)                                                                                                                                                                 atGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGGGTGGACTCCGTACCGCAGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTc                                                                                        >  2:433826/1‑232 (MQ=255)                                                                                                                                                                  tGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAAc                                                                    >  1:453442/1‑251 (MQ=255)                                                                                                                                                                       cATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGt                                                               <  1:564444/251‑1 (MQ=255)                                                                                                                                                                       cATTTTATGTAACGCCAGGGATTTCCCGATGTGGTCCACCGCATGGCGCAAATCCCGGAAAAGGTACAGATAAAGCGACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCCAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGGCGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGt                                                               <  2:166684/251‑1 (MQ=255)                                                                                                                                                                         ttttATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTgg                                                             >  1:331547/1‑251 (MQ=255)                                                                                                                                                                                 aTCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTtg                                                      <  2:381705/250‑1 (MQ=255)                                                                                                                                                                                   cGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTgg                                                   >  1:307344/1‑251 (MQ=255)                                                                                                                                                                                     ccAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCgg                                                                                          <  2:246919/210‑1 (MQ=255)                                                                                                                                                                                     ccAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCgg                                                                                          >  1:246919/1‑210 (MQ=255)                                                                                                                                                                                          gATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGTAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCTCGAAACATCGTGGCCATTTGTGGCTGAATa                                            <  1:189677/251‑1 (MQ=255)                                                                                                                                                                                           atgttcggATGTGTTCCACCGCATGGAGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCAGCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCTTGGTGTGGACACCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCTGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAg                                           <  2:256845/244‑1 (MQ=255)                                                                                                                                                                                           aTTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTcgcgcg                                                                                   >  1:480360/1‑211 (MQ=255)                                                                                                                                                                                           aTTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTcgcgcg                                                                                   <  2:480360/211‑1 (MQ=255)                                                                                                                                                                                               tCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTc                                       >  1:480586/1‑251 (MQ=255)                                                                                                                                                                                                ccGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTc                                       <  2:544218/250‑1 (MQ=255)                                                                                                                                                                                                  gATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTtgt                                                     <  1:428385/234‑1 (MQ=255)                                                                                                                                                                                                  gATGAGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGAATAACCGCGAAACATCGTGGCCATTtgtagatgggacgggaggcg                                    >  2:428385/1‑234 (MQ=255)                                                                                                                                                                                                     gtgtTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCAt                                 <  2:143936/251‑1 (MQ=255)                                                                                                                                                                                                           ccccGCATGGCGCAAATCCCGGAAAATGGACCGATAAATCTACGCAAAATTATACCGAAAGCGATCCATCGCTCGTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGGCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATa                           <  2:340801/249‑1 (MQ=255)                                                                                                                                                                                                             ccGCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCCTTTGTGGCTGACAAGCGGCGAGcctccccaagc                         >  2:417040/1‑241 (MQ=255)                                                                                                                                                                                                               gCATGGCGCAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCgg                                                                                  <  1:156743/192‑1 (MQ=255)                                                                                                                                                                                                                      gcAAATCCCGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGt                <  1:577213/251‑1 (MQ=255)                                                                                                                                                                                                                            cccGGAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTc            >  1:50422/1‑249 (MQ=255)                                                                                                                                                                                                                               ggAAAATGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTTAGccc       <  1:550355/251‑1 (MQ=255)                                                                                                                                                                                                                                gAAAATGTACCGATAAATCTACGCAAAAGTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTCAGCCCg      <  1:253515/251‑1 (MQ=255)                                                                                                                                                                                                                                    aTGTACCGATAAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTACTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTCAGCCCGtttt  >  1:427201/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                 AAAGCCACTGGTTAAATTTGCCCGCCGAATGGGGATTGAATGGCATGTACTGGTCGATGGCGATGAAGCAGGGAAGAAATATGCCGCTACGGTACGCAGCCTGTTGAATAACGATCGGGAAGCCGAACGAGAACATTTAACGGCGTTACCGGCGCTGGATATGGAACATTTTATGTATCGCCAGGGATTTTCCGATGTGTTCCACCGCATGGCGCAAATCCCGGAAAATGTACCGATGAATCTACGCAAAATTATCTCGAAAGCGATCCATCGCTCTTCCAAACCCGATCTTGCCATTGAAGTGGCAATGGAGGCAGGACGTCGTGGTGTGGACTCCGTACCGACGCTGCTGAAAAAAATGTTCTCACGCGTGCTGTGGCTGGCGCGCGGTCGCGCGGATTAACCGCGAAACATCGTGGCCATTTGTGGCTGAATAGCGTCGAGCATCTCATAGCGCCGACGGTATTCAGCCCGTTTT  >  NC_000913/917729‑918206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑