Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 134,256 G→T intergenic (+44/‑132) acnB → / → yacL AcnB/PF06062 family protein YacL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,2560GT92.9% 120.5 / 1.3 42intergenic (+44/‑132)acnB/yacLAcnB/PF06062 family protein YacL
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (17/22);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 5.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.93e-01

GGCGCGAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGG  >  NC_000913/134012‑134496
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
ggCGCGAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATgag                                                                                                                                                                                                                                            <  1:85165/251‑1 (MQ=255)
                                   gacTGTTGCGGCGCTGATTGGCAACATGCCGAAGACGGAAGAGTACAAGATCTACGTGGCGCAGGTAGATAAAAAAGCCGTTGCTCCTTACCGTTATCTGAACTTCACCCAGCTTTCTAAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGAATGCGGATTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACCAAATGCCCGGTTAATGCGATTCACGGGAATTTTTTTATTTCTACACCATCGct                                                                                                                                                                                                         >  2:523361/3‑250 (MQ=255)
                                       gTTGCGGCGCTGATTGGCAATCTGCCGACGCCGGAAGAGTACCAGACCTACGTTGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGtt                                                                                                                                                                                                      <  1:296547/250‑1 (MQ=255)
                                       gTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGtt                                                                                                                                                                                                      <  1:219608/250‑1 (MQ=255)
                                         tGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcc                                                                                                                                                                                                    <  1:543805/250‑1 (MQ=255)
                                         tGCGGCGCTGATTGGCAAACTGCCGACGACGGAAGAGTACAAGACATACGTGGCGCAGGTAGATAAAACAGCAGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGGGATTTTCCAGACTGCGGCTTAAAAAGTCAGCGCAAGCGCTGCGAATAAACGACACAATGCCCGGTTAATGAGATTCACGGGCATTTTTTTATTTCTAAACCATAGCCGTTCCg                                                                                                                                                                                                   >  2:129067/1‑251 (MQ=255)
                                                      ggCAAACGGCCGACGCCGGAATATTACCAGCCCTACGTGGCGCAGGTAGATAAACCAGCCGTTGATACTTACCGTTATCGGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc                                                                                                                                                                                       <  2:303216/250‑1 (MQ=255)
                                                          aaCTGCCGACGCCGGAAGCGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa                                                                                                                                                                                   >  1:137621/1‑250 (MQ=255)
                                                             ggCCGAGGCCGGAAGAGTACAAGACCGACTGGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGACCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGGTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTATTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAggt                                                                                                                                                                               <  2:212852/250‑2 (MQ=255)
                                                                    gCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACCGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCgtt                                                                                                                                                                         >  2:84474/1‑248 (MQ=255)
                                                                     ccGGAAGAGTAACAGACCTACGGTGCGCAGGTAAATAAAACAGCCGGTGATTCCTACCGTTATCTGAACTTCAACCCTCTTTGTCAGTTCACCGAGAAAGCCGATGGGGTGAATTTCCAGACCGCGGTTGAAAAAATCAGCGCCCGAGCTTCGCATTAACGATCCAATGCCCGGTTAATGAGACGCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATaa                                                                                                                                                                       <  2:236465/251‑1 (MQ=255)
                                                                                ccAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATg                                                                                                                                                            >  1:285759/1‑251 (MQ=255)
                                                                                  aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGgc                                                                                                                                                          <  1:248917/251‑1 (MQ=255)
                                                                                  aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGgc                                                                                                                                                          >  1:288143/1‑251 (MQ=255)
                                                                                  aGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGACACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGAGATTTTCCAGACTGCGGTTTAAAAAGTCCGCGCACGCGCTGCGCATAAACGACCCAATGCCCGGTTAATGCGATTCCCGGGCATTTTTTTATTTCTAACCCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGGTAATTACATCAATGgc                                                                                                                                                          >  2:290925/1‑251 (MQ=255)
                                                                                         cGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGc                                                                                                                                                   <  1:235376/251‑1 (MQ=255)
                                                                                               gcAGGTAGATAAAACAGCAGTTGATACTTACCGTTATCTGAACTTAAACCAGCTTTATCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAACGTCAGAGCACGCGCTGCGCATAAACGAAACAATGCCCGGTTACTGAGATTCCCGGGCATTTTTTTATTTATAAACCATCGCCGTACCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCACTGCGATTTc                                                                                                                                             >  2:385422/1‑251 (MQ=255)
                                                                                                        aaaaaCAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTg                                                                                                                                    <  2:3239/250‑1 (MQ=255)
                                                                                                                  gtttaTACTTACCGGTATCTGAACTTCATCCAGCTTTCTCAGTACTCCGAGAAAGCCGATGGGGTGATTGTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTTCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTTCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCag                                                                                                                          <  1:543310/247‑1 (MQ=255)
                                                                                                                                 tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATgg                                                                                                           >  1:170587/1‑251 (MQ=255)
                                                                                                                                 tATCTGAACTTAAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATgg                                                                                                           >  1:394869/1‑251 (MQ=255)
                                                                                                                                      gAACTTCATCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGAGTTTCCAGACTGCTGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTAc                                                                                                      <  1:526349/251‑1 (MQ=255)
                                                                                                                                      gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTAc                                                                                                      <  1:434776/251‑1 (MQ=255)
                                                                                                                                       aaCTTCAACCAGCTTTCTCAGGACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACg                                                                                                     <  2:237589/251‑1 (MQ=255)
                                                                                                                                               ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGc                                                                                                                                                   <  2:400644/197‑1 (MQ=255)
                                                                                                                                               ccAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGc                                                                                                                                                   >  1:400644/1‑197 (MQ=255)
                                                                                                                                                                 cGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTg                                                                           >  2:403588/1‑251 (MQ=255)
                                                                                                                                                                   agaAAACCGATGGTGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCTCACGCTCTGCGCATAAACGACACAATGCCCGGTTAATTAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCCCCGTTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTTCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGt                                                                         <  1:37677/251‑1 (MQ=255)
                                                                                                                                                                    gaAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGt                                                                         >  2:562450/1‑250 (MQ=255)
                                                                                                                                                                         ccGATGGAGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACGATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGt                                                                   <  2:308041/251‑1 (MQ=255)
                                                                                                                                                                             tGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATACACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTATCCGGTAAGGCTGCGATACTTACATCAATGGCGCACTGCGATTTCGGTGCATTGCCGGGAGCAGTGGCACAGACTATGGATTACGAATTTCTGCGCGATCTTACCGGAGTGGTACAGGAGCGt                                                               >  2:485702/1‑251 (MQ=255)
                                                                                                                                                                                   gATTTTCCAGACTGCGTTTTAAAAAGTCAGCGCACGCGCTTCGCATAAACGACACAATGCCCGGTTAATGCGATTCCCGTGCATTTTTTGATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCAGCGATAATTACATCAATGGCGCAATGCGATTGCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTcc                                                         <  2:478773/251‑1 (MQ=255)
                                                                                                                                                                                            gACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGATATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTTAAGGTGCGTATGTCCATGGGGCAt                                                <  1:408721/251‑1 (MQ=255)
                                                                                                                                                                                                  tgTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTg                                          >  1:327516/2‑251 (MQ=255)
                                                                                                                                                                                                  ggTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTg                                          >  1:129881/1‑251 (MQ=255)
                                                                                                                                                                                                              cAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGatat                                                                                     <  2:151405/196‑1 (MQ=255)
                                                                                                                                                                                                              cAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGatat                                                                                     >  1:151405/1‑196 (MQ=255)
                                                                                                                                                                                                               aGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTGTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGt                             <  2:137621/251‑1 (MQ=255)
                                                                                                                                                                                                                             gcATAAACGACACAATGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTg               >  2:51338/1‑251 (MQ=255)
                                                                                                                                                                                                                                  aaCGACACAAGTCCCGGTTAATGAGAATCCCGGGCATTTGTTTATTTCTAACCCAACGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCTATTGCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGAGATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTTGTTTAATGAAGAGGTgaatgt          <  2:446727/251‑4 (MQ=255)
                                                                                                                                                                                                                                    cGACACAATTCCCGGTTAAGGAGATTCCCTGGCATTTTTTTATTTATAAACCATCGCCGTTCCGCTGTTTTGTGCCGGTAAGGCTGCGATAACTGCATCAATGGCGCAATGCGATTACGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCCGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCAAGGGGCAGGAAGTTGGCGGGCACTGGTTTAATGAAGAGGTgaaagaaa        <  2:378986/251‑1 (MQ=255)
                                                                                                                                                                                                                                          aaTGCCCGGTTAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGGTTAATGAAGAGGTGAAAGAAAACCTgg  >  2:389348/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                
GGCGCGAATGTCTTCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGGCTGCGATAATTACATCAATGGCGCAATGCGATTTCGGTGCATTGCCGGGAGCAGAGGAACACACTATGGATTACGAATTTCTGCGCGATATTACCGGAGTGGTAAAGGTGCGTATGTCCATGGGGCATGAAGTGGTCGGGCACTGGTTTAATGAAGAGGTGAAAGAAAACCTGG  >  NC_000913/134012‑134496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑