Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	94,278	G→A	A371A (GCG→GCA)	murE →	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	94,278	0	G	A	100.0%	239.2 / NA	68	A371A (GCG→GCA)	murE	UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/37);  total (31/37)

TTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTG‑ACTTGCGCCGTTATG  >  NC_000913/94034‑94524                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        ttAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGt                                                                                                                                                                                                                                                   >  1:302134/1‑251 (MQ=255)       cAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGATAAAGCCTTACAGGCAGCGCGTCTGCAc                                                                                                                                                                                                                                             >  2:394039/1‑251 (MQ=255)         aGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACtg                                                                                                                                                                                                                                           >  1:300967/1‑251 (MQ=255)           gtgtggCGGTGGCGAAATTGAAGTCCGTCTGATGGCCGCTTTTAACGTCAGCAACCTGCTGTTCGCGCGGGGGACACTGTGGGCACTCGG‑ATAACCACTGGCTGATCGTCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACtgtg                                                                                                                                                                                                                                         <  2:452524/247‑1 (MQ=255)                         aaaTTGAAAGCCATCTGATGGGCGCGTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTgtgg                                                                                                                                                                                                                           <  1:552126/251‑1 (MQ=255)                              gAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGgtgtg                                                                                                                                                                                                                       <  1:168021/250‑1 (MQ=255)                                aaGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCt                                                                                                                                                                                                                    >  1:291812/1‑251 (MQ=255)                                         gATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCtgtg                                                                                                                                                                                                           <  1:225764/251‑1 (MQ=255)                                         gATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCtg                                                                                                                                                                                                             <  1:394039/249‑1 (MQ=255)                                            gggCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTgtggtg                                                                                                                                                                                                        <  1:254859/251‑1 (MQ=255)                                              gcgcTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGGTCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGCAGGGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCCCTGTGCGGGCAGGCTGGGGGGGGGCTTTGGCGGtggtggg                                                                                                                                                                                                      >  2:130210/1‑250 (MQ=255)                                               cgcTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGGGGTGTGTCTTTGGCTGGGGTGGCg                                                                                                                                                                                                     >  2:76999/1‑251 (MQ=255)                                                   ttgaaCGTCATCACCCTGCTGCTCTCGTTGGCGA‑ACTCTTTGCCCCCGGGCTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGGTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATc                                                                                                                                                                                                  <  2:151701/247‑1 (MQ=255)                                                    ttAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCGGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATcg                                                                                                                                                                                                 <  1:503954/250‑1 (MQ=255)                                                         gTCATCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATaa                                                                                                                                                                                           <  1:272199/251‑1 (MQ=255)                                                         gTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATa                                                                                                                                                                                            >  2:239245/1‑250 (MQ=255)                                                          tCAGCAACCTGCTGCTCGAGATGGCGACCCTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGACGTGTTCACTGCGCCAGGCACACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCCCTGGAAAAAGCCTTCCAGGCAGCGCGTCTGCGCTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTTGTGGGGCTcgcggtca                                                                                                                                                                                           >  2:258215/1‑246 (MQ=255)                                                           aggcagccTGCTGCTCGCGCTGGCGTCACTGTTGGACCTCGG‑CTGACACCTGGATGATCTGCGTAAAACCGCCGCGCGTCTGCATCCGGTTTGCGGTCGTATGGAAGTGTGCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATaaa                                                                                                                                                                                          <  2:480234/244‑1 (MQ=255)                                                               aaCCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTa                                                                                                                                                                                      >  1:282710/1‑250 (MQ=255)                                                               aaCCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTa                                                                                                                                                                                      >  1:138621/1‑250 (MQ=255)                                                                   tgctgcTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGGTAAAGGTAAGCGt                                                                                                                                                                                 >  2:148883/1‑251 (MQ=255)                                                                   tgctgcTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGCGGCGATCGCGATAAAGGTAAGCGt                                                                                                                                                                                 >  1:386256/1‑251 (MQ=255)                                                                    gctgctCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTc                                                                                                                                                                                <  1:397829/251‑1 (MQ=255)                                                                      gtcccgcgcgGGCGACCCGGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAACCCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCa                                                                                                                                                                              <  2:284744/247‑1 (MQ=255)                                                                       gctCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTcc                                                                                                                                                                               <  1:287391/249‑1 (MQ=255)                                                                        ctCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACt                                                                                                                                                                            >  1:190781/1‑251 (MQ=255)                                                                          cgcgcgGGGGACCCTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGa                                                                                                                                                                          <  2:529771/251‑1 (MQ=255)                                                                           gcgcTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGAt                                                                                                                                                                         <  1:1337/251‑1 (MQ=255)                                                                               tGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGCTTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACGGATgggg                                                                                                                                                                     >  2:328259/1‑250 (MQ=255)                                                                                       cTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGc                                                                                                                                                             >  1:439791/1‑251 (MQ=255)                                                                                                    g‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCt                                                                                                                                                <  1:328259/251‑1 (MQ=255)                                                                                                        aTCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTg                                                                                                                                               <  1:399289/249‑1 (MQ=255)                                                                                                        aTCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTg                                                                                                                                               <  2:289848/249‑1 (MQ=255)                                                                                                        aTCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTg                                                                                                                                               >  2:238008/1‑249 (MQ=255)                                                                                                        aTCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGa                                                                                                                                              <  1:250506/250‑1 (MQ=255)                                                                                                                  tGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCggtgg                                                                                                                                   >  1:30300/1‑251 (MQ=255)                                                                                                                  tGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGATGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAATTTGCTGGCGTGGCgggtg                                                                                                                                   >  2:523689/1‑248 (MQ=255)                                                                                                                   gATCGGCTGAAAACCGCCTCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGGTTGCTGACGTGGCggtggt                                                                                                                                  <  1:356723/251‑1 (MQ=255)                                                                                                                           gAAAACCGCCGCGCGTCTGCAACCGGTCTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTgacggacg                                                                                                                          <  2:386256/251‑1 (MQ=255)                                                                                                                            aaaaCCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCggtgggggcgggggg                                                                                                                         >  2:413154/1‑241 (MQ=255)                                                                                                                                ccgccgCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGACGGACGATaa                                                                                                                      >  1:174685/1‑250 (MQ=255)                                                                                                                                         gTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGCAGAGTTTGCTGACGGGGCGGTGGTggcggagggaaaaccagggacc                                                                                                            >  2:275316/1‑235 (MQ=255)                                                                                                                                         gTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGttt                                                                                                                                                   <  1:200979/212‑1 (MQ=255)                                                                                                                                         gTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGttt                                                                                                                                                   >  2:200979/1‑212 (MQ=255)                                                                                                                                                      ttGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGt                                                                                                <  2:473426/250‑1 (MQ=255)                                                                                                                                                                aTGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGGGGGGACGGACGATAAACCGCGTACCGAAGAACCCCGTGCCAtcatca                                                                                      >  1:468248/1‑250 (MQ=255)                                                                                                                                                                     aGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCCGTGGTGGCGATCGCGCTAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGAAGATAAACCGCGTACCGAAGAAACGAGTGCCATCATCACCGat                                                                                 >  1:412616/1‑250 (MQ=255)                                                                                                                                                                      gtgtTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGatat                                                                               <  1:148883/251‑1 (MQ=255)                                                                                                                                                                         gtCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCt                                                                            <  2:30300/250‑1 (MQ=255)                                                                                                                                                                            ccTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGc                                                                         <  1:522298/250‑1 (MQ=255)                                                                                                                                                                            aCTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATaaa                                                                                                                                                                                          >  2:249538/1‑138 (MQ=255)                                                                                                                                                                                  ccAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGGCGGACGATAAACCGCGTACCGCAGAAACGCGAGCCATCATCAACGATATTCTGGCgggg                                                                     >  1:521557/1‑248 (MQ=255)                                                                                                                                                                                   cAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGGCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATg                                                                  <  2:300967/251‑1 (MQ=255)                                                                                                                                                                                      gCAAACCGGCGGTGGTGGTGGATTCCGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTa                                                               <  2:296216/251‑1 (MQ=255)                                                                                                                                                                                        aaaCCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGa                                                             <  2:138621/251‑1 (MQ=255)                                                                                                                                                                                          aCCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATg                                                           <  1:371087/251‑1 (MQ=255)                                                                                                                                                                                             gACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCAGCAACGATATTCTGGCGGGAATGTTAGATGCCg                                                        <  1:130210/251‑1 (MQ=255)                                                                                                                                                                                                    gtggtggATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGCTGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGCCGGACGATAAACCGCGTACCGCAGAAACGCGGGCCATCATCCATGATATTCTGGCggggagtttagattgcgggaatac                                                 >  1:408570/1‑230 (MQ=255)                                                                                                                                                                                                          gATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAAACCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGGATGTTAGATGGCGGGCATGCCAAAgg                                           >  1:30018/1‑250 (MQ=255)                                                                                                                                                                                                             tACGCGCATACGCCGGATGCACTGGAAACAGCCTGACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTTCCGAAGAACCGCGTGCCATCATCAACGATAGTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGa                                         <  1:56136/250‑1 (MQ=255)                                                                                                                                                                                                                       cGCCGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGGGCCATCATCAACGATATTCTGGCGGGGATGTTTGATGCCGGACATGCCacaaggagggaaaggcgg                              >  1:511062/1‑234 (MQ=255)                                                                                                                                                                                                                          cGGATGCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGCGGGGACGGGAGATAAACCGCGTACCGAAGAAACCCGTGCCATCATCAACGGTATTTTTGCGGGGAGGTTAGGTGCAGGACACGccccaaacgttgaagggcggggt                           >  2:223640/1‑230 (MQ=255)                                                                                                                                                                                                                              ggCACTGGAAAAAGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAg                       <  2:261277/250‑1 (MQ=255)                                                                                                                                                                                                                                       aaaaGCCGTACAGGCAGCGCGGCTGCCCTGTGTGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTG‑ACtt             <  2:6737/251‑1 (MQ=255)                                                                                                                                                                                                                                        aaaGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTG‑ACTTg            <  1:350038/251‑1 (MQ=255)                                                                                                                                                                                                                                          aGCCTTACAGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTG‑ACTTgcg          <  1:377652/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aGGCAGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGGGGCGGACGATAAACCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGGATGTTAGAAGCCGGGCCAGCCCAAGAGATGGGAGGCCGGGCTGAAGGGGGGGACT‑GCGCCGTTATg  >  1:367105/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aGGCAGCGCGGCTGCACTGTGCGGGGAAGCGGTGGGGGTTCTTTGGCTGTGGTGGCGATCGCGAGAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTGCCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTTAAGCGGTG‑ACTTGCGCCGTTATg  <  2:54640/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                        TTAGCTCAAGTTGGGGCGATGGCGAAATTGAAAGCCATCTGATGGGCGCTTTTAACGTCAGCAACCTGCTGCTCGCGCTGGCGACACTGTTGGCACTCGG‑CTATCCACTGGCTGATCTGCTGAAAACCGCCGCGCGTCTGCAACCGGTTTGCGGACGTATGGAAGTGTTCACTGCGCCAGGCAAACCGACGGTGGTGGTGGATTACGCGCATACGCCGGATGCACTGGAAAAAGCCTTACAGGCGGCGCGTCTGCACTGTGCGGGCAAGCTGTGGTGTGTCTTTGGCTGTGGTGGCGATCGCGATAAAGGTAAGCGTCCACTGATGGGCGCAATTGCCGAAGAGTTTGCTGACGTGGCGGTGGTGACGGACGATAACCCGCGTACCGAAGAACCGCGTGCCATCATCAACGATATTCTGGCGGGAATGTTAGATGCCGGACATGCCAAAGTGATGGAAGGCCGTGCTGAAGCGGTG‑ACTTGCGCCGTTATG  >  NC_000913/94034‑94524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑