| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 91,922 | G→A | P170P (CCG→CCA) | ftsI → | peptidoglycan DD‑transpeptidase FtsI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 91,922 | 0 | G | A | 95.4% | 205.9 / ‑0.8 | 65 | P170P (CCG→CCA) | ftsI | peptidoglycan DD‑transpeptidase FtsI |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (28/34); total (28/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01 | |||||||||||
AAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTG > NC_000913/91676‑92168 | aaaaGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTTTTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCCTCTGCGTGAAGAATCTCGCCGTTAATAtccatccg > 1:146220/1‑251 (MQ=255) aaaGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGCATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTCTTTATCTGGCGCGTCAGGTGAACCCTGACCTGGCCGAATACATTCAAAAACTGAAACTG‑CCGGTGATTCCTCTGACTGAAGAATCTCGCCGGTATTATCCATCCgg > 2:189311/1‑251 (MQ=255) aaaGCGATTTAGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGGCGGTGGCCGCTGGGTGGCGCTGGCTAACGCGCCCCATATTCCGCTGGAACAAGCTT‑CAGCCCGCATTAACTCCAACCCCGAAGGGCGCTTTATTTCTCTGGCGCGGCAGGGGAACCCTGGCCTGGCGGACTGCATACAAAAACTGAAACTG‑GCGGGGGTTCATCTGCGGGAAAAATCTCCCCGTTCTTATCCATCCgg > 2:297977/1‑251 (MQ=255) aaaGCGATTGGGGCGGACCGGAAAGAAGGGCATGAGGCTGGCGGTATCAGCGGCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCgg < 2:84945/251‑1 (MQ=255) aGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGCATCCGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGGCTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTAGCCGTTTTTATCCATCCGGCg > 1:234444/1‑251 (MQ=255) gATTTGGGCTGACCCGAAAGAAGTGCATGCCGCTGGCGGTACCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGTATCA‑GCTTTCAGCCCGAATTAACGCCAACCCGAAAGGGCGCTTTATTTATCGGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAg < 2:353624/251‑1 (MQ=255) gACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAGACTG‑CCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTc < 2:230161/250‑1 (MQ=255) ccgaaagaattgcatatcgcgttcGGTACCAGCGTAGGTGCCCCCTGGAAGGCGCTGGCTAACTCGCTCGAGATTCCGCTGGATCA‑GCTTTCACCCCCCATTAACGCCAACCCGAAAGGGCCCTTTATTTATCTGGCGCGTCAGGTTAACCCTGACATGGCGGACTACAGCAAAATACTGAAACTG‑CCGGGGATACATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCt < 2:565596/228‑1 (MQ=255) cATGACGCTGGCGGTATCAGCGTAGGTGACCGCTGGAAGGCGCTGGCTAAAGCGCTCAATATTCCGCTGGATCC‑ACTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTTTCTGGCGCGTCAGGTGAACCCTGACATGGCGGAATACATCAAAAAACTGAAACGG‑CCGGGGATTCATCTGCGTGTAGAATCTCGCCGTTATTATCCATCCGGCGAAGGGACTGCTCCCCCCATTGGCTTTa > 2:562122/1‑250 (MQ=255) acggtggtggTAACAGCGGCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAAc < 2:63005/243‑1 (MQ=255) aaggtGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCTCTGGCTAACGCTCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAAc < 2:397174/247‑1 (MQ=255) ggCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCg < 1:220349/250‑1 (MQ=255) aTCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTc < 1:312145/250‑1 (MQ=255) ggTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTgg > 1:218932/1‑250 (MQ=255) aCCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGc > 1:221321/1‑251 (MQ=255) gAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTgaga < 1:145685/251‑1 (MQ=255) gAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTgaga < 2:404633/251‑1 (MQ=255) gcTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGtt > 1:258757/1‑251 (MQ=255) ggCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGAGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCg > 1:531397/1‑251 (MQ=255) aaCGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATa < 2:221321/250‑1 (MQ=255) cgcgCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAAt < 1:53678/251‑1 (MQ=255) gcTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACAGATCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGc > 1:498136/1‑251 (MQ=255) cTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCt > 1:421208/1‑251 (MQ=255) tCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCtt > 2:471981/1‑251 (MQ=255) cAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTAAGGTGAACCCTGACAGGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCGGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCAGTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCtt < 2:211395/250‑1 (MQ=255) cGCTGGAGCA‑GCTTGCAGCCCGCATTAACGCCAACCAGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGGGAACCCTGACATGGCGTACGACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAg < 2:218932/251‑1 (MQ=255) gCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGc < 1:521073/251‑1 (MQ=255) tGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCg > 1:184360/1‑251 (MQ=255) gCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAgc < 1:401958/251‑1 (MQ=255) cAGCCCGCATTAACGCCAACCCGAAAGGCCGTTTTATTTATCTGGCGCGTCAGGGGAACCCTGACATGTCGGACTACATCAAAAAACTGAATCTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCTTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAgcgcttt < 2:360016/251‑4 (MQ=255) aGCCCGCATTAACGCCATCCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATtg < 2:473114/251‑1 (MQ=255) gcaaccccGAAAGGGCCCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAACCTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGATGAGTTTCGAGAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTTTGCGTAAAGCCCg < 2:124161/246‑1 (MQ=255) gCCATCCCGAAAGGGCTCTTTATTTAGCTGGTGCGTCAGTTGACCCCGGACATGGCGGACTACAGCAAAAAACTGAAACTG‑CCTGGGATTCATCTGCGTGAATAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCg < 2:151940/251‑1 (MQ=255) cGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGt > 1:552613/1‑251 (MQ=255) aaaGGCCGCTTTATTTACCTGGCTCGTCAGGGGAACCCTGACATGGCGGACTACATCAAAAAACTGAACCTT‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcg < 2:106108/251‑1 (MQ=255) aGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg > 2:274620/1‑251 (MQ=255) aGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTcgcg < 1:183132/251‑1 (MQ=255) ggCGCTTTATTTATCGGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTa < 2:258757/251‑1 (MQ=255) cgcTTGATTTATCTGGCGCGTGAGGTCAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTCTCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGAAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAAt < 2:46733/251‑1 (MQ=255) tttatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACACCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGa < 2:184360/250‑1 (MQ=255) ttatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGat > 1:501778/1‑250 (MQ=255) tatCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGatat > 1:319532/1‑251 (MQ=255) tcagtggggtatggTGACCCCTGACATGCGGGACTCCAACAAAAA‑CTGTAACCGTCCGGGTATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCTCCTCAATGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAACCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATAtt < 2:115636/238‑1 (MQ=255) cTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATttc > 1:139255/1‑251 (MQ=255) gttcagcccTGACAGGGCGGATTACACTAAAAAACGCCAACTG‑CCGTGGTTTCTTCGACGTGAAGATTGGCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGATAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACa < 2:305676/245‑1 (MQ=255) aCCCTGACATGGCGGACTACATAAAAAAACGGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAg < 2:146220/251‑1 (MQ=255) aCATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTgcg > 2:206906/1‑251 (MQ=255) aTGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGCGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAGGATATTTCTTCTACTGACAGCCAGGCTgcgcc > 2:185969/1‑250 (MQ=255) cGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAAc > 1:436966/1‑251 (MQ=255) ggACTACAGCAAAAAACTGAAACTT‑CCGGGTATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCGCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAAcc < 1:376435/251‑1 (MQ=255) cATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGgcgc > 2:24728/1‑251 (MQ=255) actgaaactg‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATtg > 1:561786/1‑251 (MQ=255) ggaaacgg‑CCGGGGATTCATCTGCTGGAAGAATCTCGCCGTTATTATCCAGCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATtgat < 2:112821/250‑1 (MQ=255) aaactg‑CCGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATtgatga < 2:207250/251‑1 (MQ=255) cGGGGATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGCTTAACGCCTg > 2:44450/1‑251 (MQ=255) ggggATTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGc > 1:173583/1‑251 (MQ=255) ggATTCATCTGCGTGAAGAAGCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAg < 2:499346/251‑1 (MQ=255) aTTCATCTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGgc < 1:297977/251‑1 (MQ=255) catccgcgggaagaaactcGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTGTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCt < 2:118154/235‑1 (MQ=255) gCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGGCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTTCCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGCCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGtt < 2:234444/250‑1 (MQ=255) gTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCAACTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTAt > 1:162506/1‑251 (MQ=255) agaatctcGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGCTTGAGGGCGTTGAGAAGAGTTTCGATACATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACAGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGAAAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGCTGAACGCCTGCCGGCGCTGGTTTATcgcg > 1:394413/5‑251 (MQ=255) cGCCGTTATTATCCATCCGGCGAAGTGACTGCTCTCCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGATTTGCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACt < 1:41876/248‑1 (MQ=255) cGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaa < 1:472070/251‑1 (MQ=255) ccGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGATAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaac > 1:493470/1‑250 (MQ=255) ccGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGaac > 2:94608/1‑250 (MQ=255) atccatccGGCGAAGTGACTGCTCACCTCATTGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCTGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAATGCGGTg > 1:270520/1‑251 (MQ=255) | AAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGGTGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCA‑GCTTTCAGCCCGCATTAACGCCAACCCGAAAGGGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTG‑CCGGGGATTCATCTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCAAGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCTATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTGCAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTG > NC_000913/91676‑92168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |