Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,375,354 G→A P102S (CCT→TCT)  epmB ← lysine 2,3‑aminomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,375,3540GA98.0% 161.0 / ‑4.1 50P102S (CCT→TCT) epmBlysine 2,3‑aminomutase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (24/25);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.56e-01

GAGCAACCAGTCCAGCTCGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTC  >  NC_000913/4375121‑4375589
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
gcgCAACCAGGCCAGCTCGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTGTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGtt                                                                                                                                                                                                                            <  2:190372/249‑1 (MQ=255)
gAGCAACCAGTCCAGCTCGTGATCGTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAATCCAGACACTACGCTGTGCTGtt                                                                                                                                                                                                                            <  1:274573/251‑1 (MQ=255)
         gTCCAGCTCGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTg                                                                                                                                                                                                                   >  1:576051/1‑251 (MQ=255)
          tCCAGCTCGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTgg                                                                                                                                                                                                                  >  1:190601/1‑251 (MQ=255)
                 cGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCgg                                                                                                                                                                                                             <  1:49559/249‑1 (MQ=255)
                   tGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATAATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCCTAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACAAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCggagg                                                                                                                                                                                                          >  2:478441/1‑247 (MQ=255)
                       cTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAACCCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAAt                                                                                                                                                                                                     >  2:462179/1‑251 (MQ=255)
                         ttCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATc                                                                                                                                                                                                    <  1:169610/250‑1 (MQ=255)
                              cATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGAAGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTCTTTATGCCACAAACCAGACACTACGCTGAGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGgcc                                                                                                                                                                                              >  2:166858/1‑250 (MQ=255)
                                               cgGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCt                                                                                                                                                                             >  2:246573/1‑251 (MQ=255)
                                                         catcTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTaa                                                                                                                                                                   <  2:512660/251‑1 (MQ=255)
                                                            cgcGTCCAGTGCCGGATGAGCAGAAACATACTTAAGTGCAGTTGGACAGTTTCGCTTGTTGCACTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGAAATTTACCGCGCAGCCGCCTTTGACAAGCAAAAGCGCCCGGTTGGGGTATTGATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCAAGTGGGTCGGTGGAGAAGCCGGGCGCGATGACAAACTCAGCTTGCGAGGTAAGt                                                                                                                                                                 <  2:370094/248‑1 (MQ=255)
                                                              cGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGgcg                                                                                                                                                                                              <  2:228550/219‑1 (MQ=255)
                                                              cGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGgcg                                                                                                                                                                                              >  1:228550/1‑219 (MQ=255)
                                                                                 agcaACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGAGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGAAAGTACCTGACGCAAAAGAGGATCGt                                                                                                                                           >  2:114491/1‑251 (MQ=255)
                                                                                     aCATACTAAAGTGCAGTTTGCCAGTTCCGCTTGTTGCCCTGATTTACGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCTTcctg                                                                                                                                       <  2:236857/251‑3 (MQ=255)
                                                                                         aCTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGGGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTg                                                                                                                                   >  2:468260/1‑251 (MQ=255)
                                                                                                 gCAGTTGGCCAGTGACGCTTGTTGCCCTGATTTTCGGCTTAGGGGAAGTGACGAAGGAAGCAATAGCGGCAATTTACCGCGCAGCCTCCTGTGACCAGCAAAAGCGCCCGGTTGTGGTATTTAGGCAACAAACCAGACACTTCGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTtctc                                                                                                                           <  2:397936/251‑1 (MQ=255)
                                                                                                  cAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTcc                                                                                                                          >  1:464746/1‑251 (MQ=255)
                                                                                                        gCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGcg                                                                                                                     >  1:418127/1‑250 (MQ=255)
                                                                                                                cGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATaaa                                                                                                            <  1:468260/251‑1 (MQ=255)
                                                                                                                 gctggttgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTGACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACGACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAag                                                                                                                                                   <  2:421835/207‑1 (MQ=255)
                                                                                                                 gCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAag                                                                                                                                                   >  1:421835/1‑211 (MQ=255)
                                                                                                                     gttgCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAg                                                                                                        <  2:264213/250‑1 (MQ=255)
                                                                                                                             gATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACACGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAATAGGATCGTCCGGATTGCCTTTCTCCATGCGCTCGATAAATGAGCGGGGcac                                                                                                >  2:236083/1‑250 (MQ=255)
                                                                                                                               ttttCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACg                                                                                             >  1:567011/1‑251 (MQ=255)
                                                                                                                                           gggAAGTGACGACGGAAGAAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTGTGCCACAAACCCGACACTCCGCTGAGCTGTTCTTCCAGTGGGTCGGTGGAAAATCCGGGCGCGATGACACACTCCTCTTGCGCGGTAGGTACCTGACGCACAAGAGGATCGTCCGGATTGCCTTTCTCCATTCGATCGATAAATGAGCGGGGCACACGCAGGGCcaaa                                                                                   >  2:349952/1‑249 (MQ=255)
                                                                                                                                                 ggacgacGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCttttt                                                                           <  1:366583/250‑1 (MQ=255)
                                                                                                                                                        ggAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTg                                                                    >  2:77985/1‑251 (MQ=255)
                                                                                                                                                         gAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGc                                                                   <  2:44770/251‑1 (MQ=255)
                                                                                                                                                                    ggCAATTTCCCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGGGGTATTTATGCAACAAACTAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCGCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAAc                                                        <  2:111545/251‑1 (MQ=255)
                                                                                                                                                                           tACCGCGCAGCCGCCTTTGACCAGCAAACGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTCAGTACCTGACGCACAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCGGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTCACAGttttt                                                 >  2:571330/1‑251 (MQ=255)
                                                                                                                                                                               gcgcAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTtcctc                                             <  1:246573/251‑1 (MQ=255)
                                                                                                                                                                                cgcAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGGGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTtcctcc                                            <  2:418127/251‑1 (MQ=255)
                                                                                                                                                                                 gcAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCcg                                           <  1:120367/251‑1 (MQ=255)
                                                                                                                                                                                      cgccTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCt                                      <  1:52385/251‑1 (MQ=255)
                                                                                                                                                                                        ccTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCtat                                    >  2:160398/1‑251 (MQ=255)
                                                                                                                                                                                          tttGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTatat                                  >  1:300435/1‑251 (MQ=255)
                                                                                                                                                                                           ttGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATAtt                                 >  1:428894/1‑251 (MQ=255)
                                                                                                                                                                                               ccAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGTTCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCTCACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCTTCTATATTCaaa                             <  2:563145/251‑1 (MQ=255)
                                                                                                                                                                                                   cAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGAc                         <  1:349952/251‑1 (MQ=255)
                                                                                                                                                                                                    aaaaGCGCCCGTTTGTGGTATTTTTGCAACAACCCAGACGCTTCGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTTCCCGACGCAAAAGAGGTGCGTCCGGATTTACTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTTGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGGCTATATTCAAAAGACg                        <  2:314820/251‑1 (MQ=255)
                                                                                                                                                                                                    aaaaGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACg                        >  1:191448/1‑251 (MQ=255)
                                                                                                                                                                                                       aGCGCCCGGTTGTGGTATTTATGCAACAAACCAGACACTCCGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAAGCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAg                     <  2:297122/251‑1 (MQ=255)
                                                                                                                                                                                                                  gtgGTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTtcatc            >  1:61743/1‑249 (MQ=255)
                                                                                                                                                                                                                    ggTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGaa                   >  1:477015/1‑240 (MQ=255)
                                                                                                                                                                                                                    ggTATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGaa                   <  2:477015/240‑1 (MQ=255)
                                                                                                                                                                                                                      tATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATc      >  1:283332/1‑251 (MQ=255)
                                                                                                                                                                                                                      tATTTATGCAACAAACCAGACACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATAGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATc      >  1:526722/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTGCAACAAACCAGACACATCGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCGGGGCGCGAGGACAAACTCATCTTGCGAGGTAAGGACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGTTCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCGGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTc  <  2:76723/250‑1 (MQ=255)
                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                           
GAGCAACCAGTCCAGCTCGTGATCTTTCGCCATCAGCGGATCGCCGCCGGAGAAAATCATCTCGTCCAGTTCCGGATGCGCAGCAACATACTCAAGTGCAGTTTGCCAGTTACGCTTGTTGCCCTGATTTTCGGCATAGGGGAAGTGACGACGGAAGCAATAGCGGCAATTTACCGCGCAGCCGCCTTTGACCAGCAAAAGCGCCCGGTTGTGGTATTTATGCAACAAACCAGGCACTACGCTGTGCTGTTCTTCCAGTGGGTCGGTGGAGAATCCGGGCGCGATGACAAACTCATCTTGCGAGGTAAGTACCTGACGCAAAAGAGGATCGTCCGGATTGCCTTTCTCCATGCGATCGATAAATGAGCGGGGCACACGCAGGGCAAAAAGCTTTTTGGCGCTGCGTCCGGCTAACAGTTTTTCCTCCGCGTCTATATTCAAAAGACGCAGAAGTTCATCAGGATCGGTC  >  NC_000913/4375121‑4375589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑