Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,993,531	T→C	R793R (CGT→CGC)	cyaA →	adenylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,993,531	0	T	C	100.0%	219.1 / NA	62	R793R (CGT→CGC)	cyaA	adenylate cyclase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/35);  total (27/35)

CATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTC  >  NC_000913/3993286‑3993775                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     cATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGACGGCTTTGCCCGCGGAGGGATCATCCAGTTCTTTTTTGAAGAAACGCAAGACGAGAATGGCTTTTATATCTACATTCTCGACGAAAACAACCCGGTTTAGGTATATTACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTCCAG‑CGCTTCTACTCGTCATCGCATGACCGcttttc                                                                                                                                                                                                                                                 >  1:549312/1‑249 (MQ=255)  aTAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTAcc                                                                                                                                                                                                                                                <  1:444998/251‑1 (MQ=255)       aaaCTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACgg                                                                                                                                                                                                                                           <  1:234855/251‑1 (MQ=255)                ggCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCtt                                                                                                                                                                                                                                  <  2:198706/251‑1 (MQ=255)                ggCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGGGGCCGGCTTTGCCAGCGAAGGGGTCAACCAGGTCTTTTTCGAAGAAACCCAAGACGAGAATAGCTTTAATATTTAACTTCTCCACGAAAGCAACCCGGGTGAGGGATATCCACACTGCGCAGGCCGCAAAAAGGAGATGGGACGTGATGTC‑AGTCGGCTCTCCTTCTCATCCGATGAACGCCTTACATACGGCTCAAGCtt                                                                                                                                                                                                                                  >  1:432030/1‑251 (MQ=255)                         gagcttgTTGAAATCAATCTCGTCAAATCACCGGCGGAGGTGGATGTCTCTTCCAGCGAAGGGTGCATCCTGTTCTTTTTCGAAGAAACGCAAGACGAGAAGGGCTTTAATATCTACATTCTCGACGAAAGAAACCGGGTTGAGTTAGAGCACCACAGAGGAGTGTGCAAATAGGAGCTGGTACGTGATGTC‑GGTCGCTTCTACTCGTCATCGCATGACCGCTTGACCTACGGCTCAAGCTtcattatctt                                                                                                                                                                                                                         <  2:154121/245‑7 (MQ=255)                         gTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCTCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACtt                                                                                                                                                                                                                         <  1:270140/251‑1 (MQ=255)                                     aCCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCa                                                                                                                                                                                                             <  1:152043/251‑1 (MQ=255)                                      ccAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAg                                                                                                                                                                                                            <  1:432287/251‑1 (MQ=255)                                                 aaaTTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGAt                                                                                                                                                                                                 <  1:395412/251‑1 (MQ=255)                                                   aTTACCGGCGGGGGTTGACGGCTTTGCCAGCGAAGGGATCATCCAGTACTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATtg                                                                                                                                                                                               <  2:456616/251‑1 (MQ=255)                                                         ggcggTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGTGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAgg                                                                                                                                                                                         <  2:367381/251‑1 (MQ=255)                                                           gggtggtGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTGTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGtt                                                                                                                                                                                       <  2:162682/250‑1 (MQ=255)                                                           cggTGGTGGACGGCTTTACCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGtt                                                                                                                                                                                       >  1:123526/1‑251 (MQ=255)                                                               ggtggACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATg                                                                                                                                                                                   >  2:77987/1‑251 (MQ=255)                                                                                       gATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGAttccgttcc                                                                                                                                                           <  1:77987/251‑1 (MQ=255)                                                                                           atcCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGcac                                                                                                                                                       >  1:480891/1‑251 (MQ=255)                                                                                                   cTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCt                                                                                                                                                >  1:510796/1‑250 (MQ=255)                                                                                                           aagaaACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAAc                                                                                                                                       >  1:80608/1‑251 (MQ=255)                                                                                                                  gCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATgccgc                                                                                                                                >  1:182353/1‑251 (MQ=255)                                                                                                                   cAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGAATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCAAAAAATCTATCGGTAACATgccgcc                                                                                                                               >  2:84769/1‑251 (MQ=255)                                                                                                                     aGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTTTCGGAAACCTGCCGCCTg                                                                                                                             >  2:49752/1‑251 (MQ=255)                                                                                                                               ggCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAggg                                                                                                                   >  2:34823/1‑250 (MQ=255)                                                                                                                                    tAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCAc                                                                                                               <  1:196275/250‑1 (MQ=255)                                                                                                                                    tAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACg                                                                                                              >  1:476875/1‑251 (MQ=255)                                                                                                                                    tAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACg                                                                                                              >  2:140674/1‑251 (MQ=255)                                                                                                                                     aaTATCTTCATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCCCTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTCCGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACg                                                                                                              <  2:432030/250‑1 (MQ=255)                                                                                                                                     aaTATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACg                                                                                                              >  2:8009/1‑250 (MQ=255)                                                                                                                                        atCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATAc                                                                                                          >  1:505113/1‑251 (MQ=255)                                                                                                                                             cATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGCTAcgccgc                                                                                                     >  2:564450/1‑251 (MQ=255)                                                                                                                                                 ctcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTAtt                                                                                                 >  1:103882/1‑251 (MQ=255)                                                                                                                                                 atcGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCCCGATACGCCGCTAtt                                                                                                 >  2:480394/2‑251 (MQ=255)                                                                                                                                                   cgacgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTAtt                                                                                                 <  1:34823/249‑1 (MQ=255)                                                                                                                                                   cgacgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTa                                                                                                <  1:485019/250‑1 (MQ=255)                                                                                                                                                      cgaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGc                                                                                            >  1:206373/1‑251 (MQ=255)                                                                                                                                                       gaAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGCGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACAAGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGc                                                                                            >  1:165886/1‑250 (MQ=255)                                                                                                                                                           acAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAatat                                                                                       <  2:261791/250‑1 (MQ=255)                                                                                                                                                               ccGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACAGGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAttttt                                                                                   <  1:170466/251‑1 (MQ=255)                                                                                                                                                                   gTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtg                                                                               <  1:578490/251‑1 (MQ=255)                                                                                                                                                                     tGAGGTATACCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtga                                                                              <  2:541245/250‑1 (MQ=255)                                                                                                                                                                      gAGGTATATCACCACTGCGAAGGCAGCAAAGAGGGGCTGGTACGTGACGTC‑AGTCGCTTCTACCCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTGTTCGtgatg                                                                            <  2:525438/251‑1 (MQ=255)                                                                                                                                                                      gAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatg                                                                            <  1:323548/251‑1 (MQ=255)                                                                                                                                                                       gggTATATCCCCTCGGCGAAGGCAGCAAAGAGGGGCTGGTTCGTTACGTC‑AGTCGCTTCTACTCGGCATCGCAGGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga                                                                           <  2:320768/250‑1 (MQ=255)                                                                                                                                                                           atatCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATAGTTTTCGTGATGAACg                                                                        <  1:414575/250‑1 (MQ=255)                                                                                                                                                                             atCACCACTTCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCGTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTg                                                                      <  2:206373/250‑1 (MQ=255)                                                                                                                                                                              tCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGcc                                                                    >  1:530795/1‑251 (MQ=255)                                                                                                                                                                                   acTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGaaa                                                               <  1:505781/251‑1 (MQ=255)                                                                                                                                                                                      gCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGCCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAg                                                              <  2:248786/249‑1 (MQ=255)                                                                                                                                                                                       cGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCg                                                            >  1:218236/1‑250 (MQ=255)                                                                                                                                                                                        gAAGGCAGCAAAGAGGAGCTGGTACGTGACGGC‑AGTCGCTTCTACGCTTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTTAATGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAg                                                          <  2:223752/251‑1 (MQ=255)                                                                                                                                                                                            gcagcaAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCtt                                                      <  1:564450/251‑1 (MQ=255)                                                                                                                                                                                            gcagcaAAGAGGAGCTGGTACGTGACGTC‑AGGCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCtt                                                      <  1:176677/251‑1 (MQ=255)                                                                                                                                                                                                caAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATcc                                                  <  2:510796/251‑1 (MQ=255)                                                                                                                                                                                                     aggagCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCAt                                             >  1:438857/1‑251 (MQ=255)                                                                                                                                                                                                      ggagCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACg                                                                        >  1:537308/1‑223 (MQ=255)                                                                                                                                                                                                      ggagCTGGTACGTGACGGC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACg                                                                        <  2:537308/223‑1 (MQ=255)                                                                                                                                                                                                                   gACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGATTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGAATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCgg                                >  1:573270/1‑250 (MQ=255)                                                                                                                                                                                                                     cGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGaa                              >  1:146/1‑250 (MQ=255)                                                                                                                                                                                                                        c‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga                                                                           >  2:330533/1‑202 (MQ=255)                                                                                                                                                                                                                        c‑AGTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGtgatga                                                                           <  1:330533/202‑1 (MQ=255)                                                                                                                                                                                                                           gTCGCTTCTACTCGTCATCGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAactgac                        <  2:549312/251‑1 (MQ=255)                                                                                                                                                                                                                                             cGCATGACCGCTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCCGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCctgctg      >  2:548201/1‑251 (MQ=255)                                                                                                                                                                                                                                                  gACCGCTTTACCTACGGCTCAAGCTGCAGCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCTCACAAAATCTATCGGTAACATGCCGCCGGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTc  <  1:22008/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     CATAACAAACTGCACGGCCTGTCAGTGCAGGTTGAAACCAATCACGTCAAATTACCGGCGGTGGTGGACGGCTTTGCCAGCGAAGGGATCATCCAGTTCTTTTTCGAAGAAACGCAAGACGAGAATGGCTTTAATATCTACATTCTCGACGAAAGCAACCGGGTTGAGGTATATCACCACTGCGAAGGCAGCAAAGAGGAGCTGGTACGTGACGTC‑AGTCGCTTCTACTCGTCATCGCATGACCGTTTTACCTACGGCTCAAGCTTCATCAACTTCAACCTGCCGCAGTTCTATCAGATTGTGAAGGTTGATGGTCGTGAACAGGTGATTCCGTTCCGCACAAAATCTATCGGTAACATGCCGCCTGCCAATCAGGATCACGATACGCCGCTATTACAGCAATATTTTTCGTGATGAACGTGCCGGAAAGCGAGGCTTATCCGGCATGCAATCTTAGCGGAAACTGACTGTTTCACCCGCCTGCTGCGTC  >  NC_000913/3993286‑3993775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑