Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	157.0 / NA	48	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/26);  total (22/26)

ATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCA  >  NC_000913/3387797‑3388276                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               aTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcggc                                                                                                                                                                                                                                          <  1:318318/251‑1 (MQ=255) aTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcgg                                                                                                                                                                                                                                           >  1:4638/1‑250 (MQ=255)               atacccGGCCAGCCCCCACGCATACGAGTTTTCTATGCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATcgccg                                                                                                                                                                                                                            <  2:357591/247‑1 (MQ=255)                taACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGCGGATAGCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAGTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGAGGCAACAAAcgccgc                                                                                                                                                                                                                           >  1:1888/1‑251 (MQ=255)                 aaCCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCt                                                                                                                                                                                                                          <  1:490234/251‑1 (MQ=255)                 aaCCGGCAAGACCCAACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATAGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGAGCCGCTGATGCGCAAAAAGCAACGCTAGCGAATAGCGCCAGAATACGGTTCACATCCCGCAGCAAAGGCAGGACAGGCGGCAACAATcgccgcc                                                                                                                                                                                                                          >  2:380463/1‑250 (MQ=255)                        cAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGt                                                                                                                                                                                                                    >  1:175445/1‑250 (MQ=255)                        cAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGAAACAATCGCCGCTGTCAGt                                                                                                                                                                                                                    >  2:6976/1‑250 (MQ=255)                             cccACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCCATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATAAGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTAccccc                                                                                                                                                                                                              >  2:434284/1‑248 (MQ=255)                                                   tCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTgg                                                                                                                                                                                         <  2:431997/250‑1 (MQ=255)                                                           gCGAGGATAGCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAAGCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAg                                                                                                                                                                                <  1:224576/251‑1 (MQ=255)                                                               ggATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGGGGCGTTTCCAGCTGGAAGTGAAAGCcaa                                                                                                                                                                            <  1:212351/251‑1 (MQ=255)                                                                gATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaac                                                                                                                                                                           <  1:347735/251‑1 (MQ=255)                                                                gATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCCGCTGGAAGTGAAAGCcaac                                                                                                                                                                           >  2:157567/1‑251 (MQ=255)                                                                     ccAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaaca                                                                                                                                                                      <  1:65918/251‑1 (MQ=255)                                                                     ccAGGTACAAAAACCGGCCCAGAGACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAAGACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCTAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaaca                                                                                                                                                                      <  1:350417/251‑1 (MQ=255)                                                                                        cAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAGGCCAACAAACAGCGCCAGTACAATGGCggg                                                                                                                                                   >  1:193764/1‑250 (MQ=255)                                                                                               aGCACCCCAGAATCAACAATAATGGTGCGCGGATCATCGCAAAGATGACCACCAGTCCGGCAATCCAGCCAATAAATGGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGt                                                                                                                                           <  2:310308/251‑1 (MQ=255)                                                                                               aGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCCAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACCATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGACACCCAACACACAgcccccgtccactggccgtctccctt                                                                                                                                            >  2:272149/1‑227 (MQ=255)                                                                                                            catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGCCCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGGTTCCAGCTGGAAGGGAAAGCCAACAAACAGAACCAGTACAATGGCggacacccggtttatcgcaa                                                                                                                                  >  1:535982/1‑230 (MQ=255)                                                                                                            catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaa                                                                                                                                                                            <  2:238155/206‑1 (MQ=255)                                                                                                            catcaATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCcaa                                                                                                                                                                            >  1:238155/1‑206 (MQ=255)                                                                                                                aataatGGGGCGCGGATAACCGAAATGAGGAGCACAAGGCCGGAAATACAGCCAATAAATGGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAATGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTCTACCGCAAAGCGAat                                                                                                                          <  2:1888/251‑1 (MQ=255)                                                                                                                      ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCAGGAAGTGAAAGCCAAGAATCAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTg                                                                                                                   <  1:48123/251‑1 (MQ=255)                                                                                                                       gcgcgcGGATCATCGCAAGGATGATCACAGGCCCGGAAATACAGCCAATAAATTGGCCGATGATGCGCAAAAAGCCACGATAGCGAAGAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTgg                                                                                                                  <  2:338294/249‑1 (MQ=255)                                                                                                                              gaccatcGCAATGATGACCACCAGGCCGGCAAAACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGGACCGCCCAGCGGGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGTTTTACCGCAAAGCGAAGAT‑GTTGGTTAGCaa                                                                                                           <  2:535982/248‑1 (MQ=255)                                                                                                                                       aaTGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGaaaa                                                                                                  <  1:177277/251‑1 (MQ=255)                                                                                                                                           atgacCACCAGGCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATAcc                                                                                              <  2:126546/251‑1 (MQ=255)                                                                                                                                               cccccAGGCCGGCAATACAGCCAAGAAATGTGCTGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAAGACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATc                                                                                          <  2:314244/248‑1 (MQ=255)                                                                                                                                                cACCAGTCCGGCAATACCGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAACCAGGGCCAGTACAATGGCGGTCGCC‑AGCTTTACCCCAAAACGGAAAT‑GTTCGTTaaaaattggggaaatacacatcg                                                                                         >  1:242363/1‑229 (MQ=255)                                                                                                                                                  ccAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGATGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTg                                                                                       <  1:434284/251‑1 (MQ=255)                                                                                                                                                       ccGGCAAGACAGCCAATAAATTCGCCGATTATGCCCAAAAAGCCACGATAGCGAATTGCACCAGAATACGGTTCACCTCCCGCAGCAAGGGCCGGACCGGCGGCTTCTATCGTCGCTGTCTGTACCGCCCAGCGTGTCGTTTCCAGCTGGAAGTGAAATCCAACAAACAGCGCCAGTACTATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTa                                                                                   <  1:560138/250‑1 (MQ=255)                                                                                                                                                             aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCACCAAACAGCGCCAGTACAATGGCGGGCGCC‑AGGTTTACCGCAAAGCGAAATT‑GTTGGATAGCAATGGAGAAAATACCCATCGGGAtttaacaaa                                                                             >  1:406377/1‑243 (MQ=255)                                                                                                                                                                 aGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCCAAGGAGAAAATACCCATCGTGATTAACCAAAATCAc                                                                        >  1:287975/1‑251 (MQ=255)                                                                                                                                                                  gCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACCATGGCGGTCGCC‑CGTTTTACCGCAAAGCGAATAG‑GTTGGTTCGCAATGGAGAAAAGACCCATCGGGATTAAACAAACTCaac                                                                       >  1:104085/1‑249 (MQ=255)                                                                                                                                                                    cAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCa                                                                    <  1:42431/251‑1 (MQ=255)                                                                                                                                                                            gtgCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATg                                                             <  1:232433/250‑1 (MQ=255)                                                                                                                                                                             tgCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGGTGCC‑AGGTTTACCCCCAAACGGACATTGTGGGTT‑GCCATTGGGAAAATACCCATCATGATTAACCacaatccccccgggggtggg                                                            >  2:368200/1‑232 (MQ=255)                                                                                                                                                                                  atgatgCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTGCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCAtt                                                      <  2:231356/251‑1 (MQ=255)                                                                                                                                                                                         gcAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGGGAAAATACCCATCGTGATTAACCAAA‑ACACCGCAGGCGATGGGCCATTTTACggg                                               >  2:205893/1‑250 (MQ=255)                                                                                                                                                                                           aaaaaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACACTGGCGGTCGCC‑CGTTTTACCGCAAAGCGAATAT‑GGTGGGTAGACATGGAGAAAATACCCATCGGGATTAACCAAACTCAC‑CCCGGCGGAGGGCCATTTTAAGga                                               >  1:103346/1‑249 (MQ=255)                                                                                                                                                                                                  cACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCATTTTTACCCCAAA‑CGAATAT‑TTTGGTTTGCAATGGGGAAAATACCCATCCTGGTTTACCAAAACCAC‑GCCAGCGGTGGGGCATTTTTCGggagaaagca                                       >  2:122017/1‑246 (MQ=255)                                                                                                                                                                                                          cGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑CGTTTTACCGCAAAGCGAATAT‑GTTGGTTTGCAATGGAGAAAATACCCATCGTGATTAACCAAAACCAC‑GCAGGCGGTGGGCCCTTTTACGGGAGAACGAATCCTGGCtt                              >  1:2168/1‑251 (MQ=255)                                                                                                                                                                                                          cGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGcgt                              <  2:116995/251‑3 (MQ=255)                                                                                                                                                                                                               aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtc                         <  2:273873/251‑1 (MQ=255)                                                                                                                                                                                                                          aCGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGt               <  1:478023/250‑1 (MQ=255)                                                                                                                                                                                                                                   cTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTTCCGCCAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATAACCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGGATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTg      >  1:353100/1‑250 (MQ=255)                                                                                                                                                                                                                                      ccGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGGGaccc  >  1:241528/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               ATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCC‑AGTTTTACCGCAAAGCGAATAT‑GTTGGTTAGCAATGGAGAAAATACCCATCGTGATTAACCAAACTCAC‑GCAGGCGATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCA  >  NC_000913/3387797‑3388276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑