Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,861	G→A	V354I (GTC→ATC)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,861	0	G	A	91.2%	162.1 / 5.7	57	V354I (GTC→ATC)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base A (28/24);  total (31/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

TGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGT  >  NC_000913/3065616‑3066106                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      tGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGc                                                                                                                                                                                                                                                  >  2:266620/1‑251 (MQ=255) ggggggAAAACCCGGTCATTCCTCCGTTTATGATGTATTCTGAAGTGCTACAGGAAAC‑GGTGGTGCAGTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGAAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGc                                                                                                                                                                                                                                                  <  2:381678/250‑1 (MQ=255)             cGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGTAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACACTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGAATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGGACTCATCCGTCGTCTTGGCGTCATCGCTCTGTACATCGGCCTGGAGTTTgct                                                                                                                                                                                                                                      >  2:448976/1‑249 (MQ=255)                   ttCCTCCGTTTATGATGTATTCGGAAGTTCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTAc                                                                                                                                                                                                                                >  1:63231/1‑251 (MQ=255)                     cctccGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGCACATCGGCCTGGAGTTTGATATTTACgg                                                                                                                                                                                                                              >  1:515244/1‑251 (MQ=255)                     cctccGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACg                                                                                                                                                                                                                               <  1:557046/250‑1 (MQ=255)                       tccGTTTATGATGTATTCGGAAGTGCTCCAGGAATC‑GGTGGTGCATTTACTGGAAACCGGAAAAATCAGCGGGGCCAGCGCCTCCAGACTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTATGAACATCGCCCTGGAGTTTGATATTTACGGGc                                                                                                                                                                                                                            >  1:535316/1‑251 (MQ=255)                                 atgTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTc                                                                                                                                                                                                                  >  2:470771/1‑251 (MQ=255)                                        cGGAAGTGCTACAGGCATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAacacac                                                                                                                                                                                                           >  2:409444/1‑251 (MQ=255)                                          gAAGTGCTTCAGGAGTCTGGTG‑TGCTTTTACTGGTAACCGGCAAAATCAGCGGGGCCACCGCCTCCAGCCTGACAAGCGCGGCCGATTCCCTGCGCAAGATGTACGACAATA‑TGTATTACTTTGCCAGCCGCATTTTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCTTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAacacac                                                                                                                                                                                                           <  2:422757/249‑1 (MQ=255)                                                                        aCTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGGCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCTTCTGGGCGTCAGCGCTCTGAACGTCGGCCTGGAGTTTGAGATGTACGGGCATGCCAACTCAACACACGGAGCCGGGGTCGAGCTGATGAACGGCATc                                                                                                                                                                             <  2:24241/250‑1 (MQ=255)                                                                                       aaTCAGCGGGGCCAGCGCCACCGGCCTGACAATCTCGGCCGACTCCCTGCGCAAGATTTACGAAAAGA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGAtt                                                                                                                                                             <  2:84962/251‑1 (MQ=255)                                                                                            gCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGaa                                                                                                                                                        <  1:120649/251‑1 (MQ=255)                                                                                                     gcgcCTCCAGCCTGACAATCTCGGCCGATTCCCGGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAAcgcg                                                                                                                                               <  2:520687/251‑1 (MQ=255)                                                                                                         cTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGc                                                                                                                                                                                                                            >  1:368700/1‑170 (MQ=255)                                                                                                         cTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGc                                                                                                                                                                                                                            <  2:368700/170‑1 (MQ=255)                                                                                                               ccTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGGGATTTTTGACGCAACGCTTATCTGTCg                                                                                                                                      >  2:513064/1‑250 (MQ=255)                                                                                                                  gACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGGCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATc                                                                                                                                   <  2:205088/250‑1 (MQ=255)                                                                                                                     aaTCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTa                                                                                                                               <  1:574922/251‑1 (MQ=255)                                                                                                                          cGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTCCTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGcc                                                                                                                          <  2:438003/251‑1 (MQ=255)                                                                                                                          cGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGc                                                                                                                           <  1:425245/250‑1 (MQ=255)                                                                                                                               gATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCCTAGGCGGCAGCGGAGATTTTGAAAGCAACGCGTCTCTGTCGGTTTTTTTGGCCCCGTc                                                                                                                     >  2:495153/1‑251 (MQ=255)                                                                                                                                   cccTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTGTCTGTCGATCTTTATGGCCCCGTCGAtt                                                                                                                 <  1:470771/251‑1 (MQ=255)                                                                                                                                   cccTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGAtt                                                                                                                 <  2:469824/251‑1 (MQ=255)                                                                                                                                      tGCGCAAGCTTTTCGA‑AATAATGGATTACTTTGCCAGCGGCATTGTGTTGCGTCCGCAGGAGATTCCCCATAACCCGGAAATCATCCGTCGTCTGTGCGTCATCGCTCTGAACATCGGCCTGTAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGTGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTg                                                                                                                <  2:160296/249‑1 (MQ=255)                                                                                                                                        cgcAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTa                                                                                                             >  1:227823/1‑250 (MQ=255)                                                                                                                                                  aCGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCAAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGACCATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCACGTCGATTGCTAAAGAAGGCa                                                                                                    >  1:328764/1‑249 (MQ=255)                                                                                                                                                    gACAATA‑TGGATTCCTTTGCCAGACGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCTGCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCATCGCGTATCGGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGAt                                                                                                <  1:322525/251‑1 (MQ=255)                                                                                                                                                        ata‑tGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCa                                                                                            <  2:52261/251‑1 (MQ=255)                                                                                                                                                              gATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGGGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAAATCTCCACcgt                                                                                       >  2:138490/1‑251 (MQ=255)                                                                                                                                                                     ttGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTTTGGCCCCGTCGATTGCTAAAGAAGGCAAAATCTCCACCGCCGTGccc                                                                                >  2:164897/1‑250 (MQ=255)                                                                                                                                                                       gCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAAt                                                                              >  1:505905/1‑251 (MQ=255)                                                                                                                                                                       gCCAGCCGCATTGCGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGGGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCACCGTCGCTTGCTAAAGAAGGCAAGATCTCCACCGTCGGGCCAAt                                                                              >  2:206229/1‑251 (MQ=255)                                                                                                                                                                           gCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTTGTTTTGTACGCCACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGATAAAGAAGGCAAGCTCTCAACCGTCGAGCCAATGTGc                                                                          >  2:218068/1‑251 (MQ=255)                                                                                                                                                                             cGCATTGTGTTGCGTCCGCAGGAGATATCCAATAACCCGGAAATAATCCGTAGTCTGGGCGTCATCGCTCTGACCATCGGCCTGGAGTTTGATATTTACGAGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGCTGAACGGCATCGTCGGCCACGGTGATTTTGAACGCAACGCGTATCTGTCCATCTTTATGGCACCTTCGATTGCTAAAGAAGGCAAGATCTCAACCGACATGCCAAtgtta                                                                          >  1:257909/1‑247 (MQ=255)                                                                                                                                                                              gCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAACTCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGc                                                                       >  1:204986/1‑251 (MQ=255)                                                                                                                                                                                aTTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGccc                                                                     >  1:262196/1‑250 (MQ=255)                                                                                                                                                                                 ttGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGCAATCATCAGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTCGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGACTTTGAACGCACAGCGTATCTGTCGATATTTATTCCCCCCTCGGTTGCTAAGGAAGACAAGATTCCAAACGTCGTGACaacatgccgaca                                                                     >  2:400699/1‑240 (MQ=255)                                                                                                                                                                                  tgtgTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATg                                                                   >  1:17294/1‑251 (MQ=255)                                                                                                                                                                                       tGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGACGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAAATCTCAACCGTCGTGCCAATGTGCAGCCATGtttct                                                              >  2:49610/1‑248 (MQ=255)                                                                                                                                                                                         cGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATcc                                                            >  1:187708/1‑250 (MQ=255)                                                                                                                                                                                            ccGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTTGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCGCAACCGTCGTGCCAATGTGCAGCCATGTTGTTcaca                                                          <  2:105070/250‑1 (MQ=255)                                                                                                                                                                                             cGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGc                                                        >  1:245920/1‑251 (MQ=255)                                                                                                                                                                                                     attcccaaataCCCGGAAATCATCCGTCGGCTGGGCGTCATCGCTCCGAAAATCGGTCTGGAGTTTGATATTTACGGGCATGCCAACCCAACACACGTAGCCGGGGGCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGGCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCTAAcacat                                                <  2:44219/241‑2 (MQ=255)                                                                                                                                                                                                              aaCCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGt                                       <  1:454395/251‑1 (MQ=255)                                                                                                                                                                                                               aCCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCTATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCCGCCATGTTGATCACAGCGAACACAGCGTCAAAg                                        >  2:535617/1‑249 (MQ=255)                                                                                                                                                                                                                 ccGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCTTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGat                                    <  1:404849/251‑1 (MQ=255)                                                                                                                                                                                                                   ggAAATCATCCGTCGTCTGGGCGTCCTCGCTCTGAACATCGGCCTGGAGTTTTATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGAGGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGatca                                  <  2:541982/251‑1 (MQ=255)                                                                                                                                                                                                                    gAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGatcat                                 <  2:515244/251‑1 (MQ=255)                                                                                                                                                                                                                      aaTCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCa                                           <  2:410186/239‑1 (MQ=255)                                                                                                                                                                                                                      aaTCATCCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCa                                           >  1:410186/1‑239 (MQ=255)                                                                                                                                                                                                                           tcCGTCGTCTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATACCAGCGAAAACAGCGTCAAAGTGATCATAACCGa                           >  2:464878/1‑250 (MQ=255)                                                                                                                                                                                                                              gtcgtcTGGGCGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTTGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACaa                       <  2:334973/251‑1 (MQ=255)                                                                                                                                                                                                                                        cGTCATCGCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCCCCGAACAAAGGATCGCCg             >  2:116233/1‑251 (MQ=255)                                                                                                                                                                                                                                               gCTCTGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTgcg      >  1:25572/1‑251 (MQ=255)                                                                                                                                                                                                                                                ctctGAACATCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTgcgc     >  1:276276/1‑251 (MQ=255)                                                                                                                                                                                                                                                   ggAACATCGGCCTGGAGTTTGAGATGTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCGTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGGGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGt  <  2:266769/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      TGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATC‑GGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTACGACAATA‑TGGATTACTTTGCCAGCCGCATTGTGTTGCGTCCGCAGGAGATTTCCAATAACCCGGAAATCATCCGTCGTCTGGGCGTCATCGCTCTGAACGTCGGCCTGGAGTTTGATATTTACGGGCATGCCAACTCAACACACGTAGCCGGGGTCGATCTGATGAACGGCATCGGCGGCAGCGGTGATTTTGAACGCAACGCGTATCTGTCGATCTTTATGGCCCCGTCGATTGCTAAAGAAGGCAAGATCTCAACCGTCGTGCCAATGTGCAGCCATGTTGATCACAGCGAACACAGCGTCAAAGTGATCATCACCGAACAAGGGATCGCCGATCTGCGCGGT  >  NC_000913/3065616‑3066106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑