Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,905,825	C→T	Q39* (CAA→TAA)	ygcG →	TPM domain‑containing protein YgcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,905,825	0	C	T	100.0%	93.2 / NA	27	Q39* (CAA→TAA)	ygcG	TPM domain‑containing protein YgcG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

ATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAACCGATG  >  NC_000913/2905601‑2906068                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aTTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAg                                                                                                                                                                                                                           <  2:83942/251‑1 (MQ=255)     aCCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGAGCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCTCAAGTTAg                                                                                                                                                                                                                       <  2:333830/251‑1 (MQ=255)        aaaaTGATATTTATTTGTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAAt                                                                                                                                                                                                                    <  2:317679/251‑1 (MQ=255)                               aTTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGt                                                                                                                                                                                              <  1:388263/250‑1 (MQ=255)                                  aGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCa                                                                                                                                                                                           >  1:328463/1‑250 (MQ=255)                                   gTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTCCGAGTAGCTTAAACTCATAAGAACAGAAAGAACTGACGCACACGTTAGAATCTATTTTAAATAATCCCCAAGTGCac                                                                                                                                                                                          >  1:347177/1‑249 (MQ=255)                                            gATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTAt                                                                                                                                                                                <  1:400843/251‑1 (MQ=255)                                                 cGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAAtt                                                                                                                                                                           <  2:470179/251‑1 (MQ=255)                                                  gAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTg                                                                                                                                                                          >  1:269642/1‑251 (MQ=255)                                                                     ccgccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACg                                                                                                                                                        >  2:282209/1‑250 (MQ=255)                                                                      agccgTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACga                                                                                                                                                       >  1:402027/2‑250 (MQ=255)                                                                                      atCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAatat                                                                                                                                       <  1:395640/250‑1 (MQ=255)                                                                                          aGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTAc                                                                                                                                  >  1:149417/1‑251 (MQ=255)                                                                                                aaTGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAagag                                                                                                                             <  1:292883/250‑1 (MQ=255)                                                                                                   gagaAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATCGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGttt                                                                                                                          <  1:512792/250‑1 (MQ=255)                                                                                                       aaTTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGAc                                                                                                                     >  1:150840/1‑251 (MQ=255)                                                                                                            ataATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTg                                                                                                                >  1:446876/1‑251 (MQ=255)                                                                                                               aTGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCg                                                                                                             >  2:241753/1‑251 (MQ=255)                                                                                                                   gcTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTa                                                                                                         <  2:69290/251‑1 (MQ=255)                                                                                                                          cATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAACGAACTGACGCACAAGTTAGCACCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAgcagctg                                                                                                  >  2:348379/1‑245 (MQ=255)                                                                                                                                                                  caaccaacTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTc                                                          >  2:421217/1‑251 (MQ=255)                                                                                                                                                                   aaccaacTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCg                                                         >  2:445599/1‑251 (MQ=255)                                                                                                                                                                                                caaGTTACGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACACACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGAAGGttt                            >  2:377687/1‑250 (MQ=255)                                                                                                                                                                                                      aCGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGAc                                                                                                                                                    >  2:205941/1‑125 (MQ=255)                                                                                                                                                                                                      aCGGATCTTACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGAc                                                                                                                                                    <  1:205941/125‑1 (MQ=255)                                                                                                                                                                                                              tACGAGTAGCTTAAACTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCGGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGgaagaa               <  2:1463/250‑1 (MQ=255)                                                                                                                                                                                                                          aaaCTCATAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTCATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAACCGATg  >  1:247737/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    ATTCACCAAAATTATATTTATTTTTTCTGTATTAGTTTTTAATGATAACGAATATAAAATTACCGATGCCGCCGTCAATTTATTTATCCAGATTTAATGAGAAATTTATAATGCGCTATTTCATACTAATGTTCACTTTCGTATGTTCCTTTGTTGCAGCCCAACCAACTATTGTCCCACAATTACAGCAACAAGTTACGGATCTTACGAGTAGCTTAAACTCACAAGAAAAGAAAGAACTGACGCACAAGTTAGAATCTATTTTCAATAATACCCAAGTGCAAATTGCCGTATTAATTGTTCCCACAACCAAAGACGAGACAATTGAACAATATGCTACAAGAGTTTTTGACAATTGGCGTTTAGGAGATGCCAAACGTAATGATGGGATACTGATCGTTGTTGCCTGGTCGGATCGCACTGTCCGCATCCAGGTAGGTTATGGGCTGGAAGAAAAGGTAACCGATG  >  NC_000913/2905601‑2906068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑