Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	89,570	A→C	intergenic (+538/‑64)	cra → / → mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	89,570	0	A	C	100.0%	148.9 / NA	43	intergenic (+538/‑64)	cra/mraZ	DNA‑binding transcriptional dual regulator Cra/DNA‑binding transcriptional repressor MraZ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (27/16);  total (27/16)

CGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAG  >  NC_000913/89333‑89804                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            cGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGTCCAGCTTTTCCTCCCCTCCGTAAACTc                                                                                                                                                                                                                                >  2:427509/1‑251 (MQ=255)                             cgcgTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGt                                                                                                                                                                                                    <  1:141598/251‑1 (MQ=255)                                     aaaCTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATa                                                                                                                                                                                             >  1:421785/1‑250 (MQ=255)                                       aCTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGACATTGTGGGGCAAAGTGGGAATAAgg                                                                                                                                                                                          >  1:401778/1‑251 (MQ=255)                                                 tataTGTGCTGTGGATAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTGTATCGTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCGCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCt                                                                                                                                                                                 <  2:125175/250‑1 (MQ=255)                                                               aaaaTAGTGAGTCATTTTAAAACGGTGATGACGCTGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCCGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGCTGGC‑CTGTTCCggggg                                                                                                                                                                 >  2:331827/1‑250 (MQ=255)                                                                             ttttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCACTCCGTAAACTCCTTTCCGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGT‑ATGTTCCggggggaaccggtagtca                                                                                                                                                    >  2:361702/1‑236 (MQ=255)                                                                              tttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTTCTCCGCTCCGTAAACTACTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGGGAGGCTGGC‑ATGTTTCGGGGAGCAACGTTAGTCAAt                                                                                                                                                  >  2:333908/1‑251 (MQ=255)                                                                               ttAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGGAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATACGGGGTGAGGCTGGC‑ATGTTCCGGGGAGAAACGTTAGACAAtc                                                                                                                                                 >  1:147013/1‑251 (MQ=255)                                                                                  aaaCGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCg                                                                                                                                              >  1:482500/1‑251 (MQ=255)                                                                                     gggTGATGCTGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGTTGCGCCCGTTGGACGTAAAATAATCAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCGTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGTC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACa                                                                                                                                           <  2:505972/250‑1 (MQ=255)                                                                                          atgaCGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCaaa                                                                                                                                      <  1:440626/251‑1 (MQ=255)                                                                                                      gATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTGCACACGTTGGACGTAAAATAAACAACGCTGATATTAGACGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCATAGTGGGAATAAGGGGTGAGGCTGGC‑ATGGTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATc                                                                                                                           >  1:207936/1‑250 (MQ=255)                                                                                                         tttttCTTACAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGt                                                                                                                        <  1:14333/250‑1 (MQ=255)                                                                                                                 aCAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGATTATCAGTGCCTAcccc                                                                                                               >  2:275061/1‑250 (MQ=255)                                                                                                                 aCAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATATCGACCGCAAAGGGCGCTTATCAGAGCCTAcccc                                                                                                               >  2:37010/1‑250 (MQ=255)                                                                                                                  cAGCTATTCATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGt                                                                                                              >  1:406374/1‑251 (MQ=255)                                                                                                                          cATAACGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCggg                                                                                                       >  1:380029/1‑250 (MQ=255)                                                                                                                           aTAACGTTAATTTGCTGCGCAAGTTGGACGTAAAATAAAAAACGCTGAGATTAGCCGTAAACATTGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTGGACAAGATTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTGCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGaa                                                                                                     <  2:421785/251‑1 (MQ=255)                                                                                                                             aaCGTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAAc                                                                                                    <  1:207538/250‑1 (MQ=255)                                                                                                                                gTTAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAgct                                                                                                >  1:467289/1‑251 (MQ=255)                                                                                                                                 ttAATTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGGGGGAATAAGGGGTGAGGATGGCCAGGTACCGGG‑AGCAACGGTAGGCAATCTCGCAAGCAACGGGCGGTTATCCATGTCTACCCGTTACCGGGAACAgcgg                                                                                               >  2:222934/1‑249 (MQ=255)                                                                                                                                    aTTTGCTGCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCtt                                                                                            >  1:448887/1‑251 (MQ=255)                                                                                                                                           gcGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAATCTTTTCCTTCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAAc                                                                                      <  1:90189/249‑1 (MQ=255)                                                                                                                                                  ttGGACGTAAAATAAACAACGCTGATATTAGCCGTAACCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCCAAGTGGGAATAAGGGCTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCACCCCGTTACCGGGCAAAGCGGCTTGGGAACGCTGCCgg                                                                              >  2:28254/1‑251 (MQ=255)                                                                                                                                                   tGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAACTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGCGAACCGCTGCTTGAGAACCCTGCCGGt                                                                             >  2:390942/1‑251 (MQ=255)                                                                                                                                                          aaaaTAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATg                                                                       <  1:361702/250‑1 (MQ=255)                                                                                                                                                               aaaCAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTg                                                                  <  1:331827/250‑1 (MQ=255)                                                                                                                                                                         gATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGCATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGGGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGAc                                                        >  1:542389/1‑250 (MQ=255)                                                                                                                                                                           tatTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTTTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAGTTGTGAGGCAAGGTGGGAATAAGGGGTTAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCACTCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTCCCGGGCACAGATGCTTGAGACAGCTGCCAGTCAAATGGCTTGCCCCATTGACAtt                                                     >  2:89352/1‑251 (MQ=255)                                                                                                                                                                            atTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACAtt                                                     <  1:79696/250‑1 (MQ=255)                                                                                                                                                                                 ccGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCAc                                               >  1:484140/1‑251 (MQ=255)                                                                                                                                                                                   gTAAACATCGTGTTGTTTACCTCGGTAGGCCTTGGGACTGGCTTGCTAAGCGTTTCCTCCGCTCCGTAAACTCCGTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGTAAAGTGCGCTTATCAGGGCCTACCCGTTTCCGGGAACAGCTGTTTGAGAACGCCGCAGGTCAAAGGGTTTGCACCATTGACATTCATAAccc                                             <  2:31424/251‑1 (MQ=255)                                                                                                                                                                                   gTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCAccc                                             <  1:525989/251‑1 (MQ=255)                                                                                                                                                                                       aCATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCCACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGGGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATccaccgttc                                         >  2:316991/1‑243 (MQ=255)                                                                                                                                                                                           cGGGTTTTTTACCTCGGTATGCCTTGAGACTGGCTTGACAAGCTTTTCCTCCGCACCGTAAACTCCCTTCCGTGGGCAATTGTGGGCCAAAGAGAGAACAAGGGGTGACGCTGGC‑ATGTTCCGGCGAGCAACGGTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTCCCTACCCGTTACCGGTAACAGCTGCTTGCGAACGCTGCCCGTCCAATGTATTGCACCCTTGACATTCCTCACCCGTGCctgc                                     >  1:552882/1‑251 (MQ=255)                                                                                                                                                                                                       cTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATATCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATGCATCACCCGTGCCTGCTGCTTTAccccc                         >  1:213481/1‑251 (MQ=255)                                                                                                                                                                                                          ggTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTTCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCcctgc                      >  2:283320/1‑251 (MQ=255)                                                                                                                                                                                                                          ggCTTGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTAt      <  2:448887/251‑1 (MQ=255)                                                                                                                                                                                                                              tGACAAGCTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCg    <  1:91977/249‑1 (MQ=255)                                                                                                                                                                                                                              tGACAAGCTTTTCCTCCGATCCGTAAACTAACTTCCGTGGGACATTGTGGCGCACAGTGGGAATACGGGGACAGGCTGGC‑ATGTTCCGGGGAGCAACGCTAGTCACTCTCGCCAGCAAAGGGCGCTTATCAGTGCCTACCCGTTAACGGGAACAGCTGCTTGAGAACGCTTCCGGTCAAATGGTTTGCACCATTGACATTCATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGGTTGGGAAATTATCgtg  >  2:246295/1‑249 (MQ=255)                                                                                                                                                                                                                                     cTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCACATGGTTTGCa                                                                >  1:298039/1‑182 (MQ=255)                                                                                                                                                                                                                                     cTTTTCCTCCGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTACCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCa                                                                <  2:298039/182‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            CGGGGAAGATATGTCCTAAAATGCCGCTCGCGTCGCAAACTGACACTTTATATTTGCTGTGGAAAATAGTGAGTCATTTTAAAACGGTGATGACGATGAGGGATTTTTTCTTACAGCTATTCATAACGTTAATTTGCTTCGCACGTTGGACGTAAAATAAACAACGCTGATATTAGCCGTAAACATCGGGTTTTTTACCTCGGTATGCCTTGTGACTGGCTTGACAAGCTTTTCCTCAGCTCCGTAAACTCCTTTCAGTGGGAAATTGTGGGGCAAAGTGGGAATAAGGGGTGAGGCTGGC‑ATGTTCCGGGGAGCAACGTTAGTCAATCTCGACAGCAAAGGGCGCTTATCAGTGCCTACCCGTTATCGGGAACAGCTGCTTGAGAACGCTGCCGGTCAAATGGTTTGCACCATTGACATTTATCACCCGTGCCTGCTGCTTTACCCCCTGCCTGAATGGGAAATTATCGAG  >  NC_000913/89333‑89804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑