Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,817,850	G→A	noncoding (11/77 nt)	argQ ←	tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,817,850	0	G	A	87.5%	114.8 / 6.1	49	noncoding (11/77 nt)	argQ	tRNA‑Arg
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (17/25);  total (21/28)
Fisher's exact test for biased strand distribution p-value = 6.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.51e-01

GAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAT  >  NC_000913/2817603‑2818066                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                         gAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGTATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATACGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                                                                                                                                                                       <  1:146838/251‑1 (MQ=255)  aGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCCAGGTTCGAAGCCGAGTACTCAATCCAGCTGAACTAc                                                                                                                                                                                                                      >  2:329107/1‑251 (MQ=255)   gTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCTGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACg                                                                                                                                                                                                                     >  1:535506/1‑251 (MQ=255)   gTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACg                                                                                                                                                                                                                     >  1:407774/1‑251 (MQ=255)     tATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCAGCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACgg                                                                                                                                                                                                                    <  2:77844/250‑1 (MQ=255)      aTTTAGCAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGTGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCCCTATCTCTTCGATCCCTTCATTGCTGAAGGTTACGGAGAATATTGTGCATCCGGGAGGATTCGAACCTCCGACCTCTCGGTTCGTATCCGGGTACTCTATCCATCTGAGCTACTGAt                                                                                                                                                                                                                  >  1:411475/1‑251 (MQ=255)             aaTGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCgg                                                                                                                                                                                                           >  1:371465/1‑251 (MQ=255)               tGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTCCGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGCGTACTCTATCCAGCTGAACTACGGATGCATCggg                                                                                                                                                                                                          >  2:151755/1‑250 (MQ=255)                 agaTATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCAATGACGTTCGCCCTTAGGGCCGTTGCTGGTGCACCGTTATTCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATCTGGAGCATCCGGGAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAACCTATCCAGCTGACCTACGGATGCATAGGGaa                                                                                                                                                                                                        >  1:277942/1‑250 (MQ=255)                       ttttGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaacttac                                                                                                                                                                                                  <  2:174812/250‑6 (MQ=255)                        tttGAAGGAAATTTTTTGGAGAAGATGGTGCTTCGGGGAGGATGACTCACTGACTTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCGTCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTCCGCTATCCAGCTGAACTACGGATGCATCGGTAAAGCTTAt                                                                                                                                                                                                 <  2:536662/250‑1 (MQ=21)                          tGAAGGAAATTTTTTGGCGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCCTTGCTGAAGGTTACGGGGAATATGGTGCATCCGGGAGGATTCGGCCCTCCGCGCGATCGGTTCGTAGCAGAGTCATCTAGCCAGCTGAACCACGGATGCATCGGGAACACTTATTcc                                                                                                                                                                                              >  2:534103/1‑250 (MQ=255)                          tGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTc                                                                                                                                                                                               <  2:182824/250‑1 (MQ=255)                                      tttGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATATATTCGCTTCCTTCATTGCTGAGGGTTACGGGGAATATGGAGCATCCGTGAGGATTCAAACCCTCGACCACTCGGTTCGTAGCCGCTGCCTATACCCAGCTGAGCTACGGCTGCATCGGGaaattttatttatcgtaagttttt                                                                                                                                                                                  >  2:496619/1‑230 (MQ=255)                                      tgtggggaagaTGGTGCATCCGGGAGGAGGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCGTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGattt                                                                                                                                                                                   <  2:324571/244‑3 (MQ=255)                                        gggAGAAGATGGGGCATCCGGGAGGATGACTCACTTACGTTCGCCCTCCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGa                                                                                                                                                                                <  2:32369/250‑1 (MQ=255)                                         ggAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTTGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGACGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCTCGCTGAACTACGGATGCATCGGGACAACtgtttcactgtctatttttgt                                                                                                                                                                                >  2:503552/1‑230 (MQ=18)                                            gaagaTGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATAcc                                                                                                                                                                            <  1:52217/251‑1 (MQ=255)                                                 tGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTTTTCTACTGCTGATATTGATACCGctac                                                                                                                                                                       >  2:390278/1‑251 (MQ=255)                                                      cagacGGGAGGATGACTCACTGGCGTTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTATCCTGCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTTGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAg                                                                                                                                                                  <  2:16925/247‑1 (MQ=255)                                                         ccGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCGCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCAGCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCa                                                                                                                                                               <  2:30096/251‑1 (MQ=255)                                                                 aTGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTAATAAAGCCATATCAAtt                                                                                                                                                       >  2:353496/1‑251 (MQ=255)                                                                   gACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTa                                                                                                                                                      >  1:374345/1‑250 (MQ=255)                                                                   gACTCACTGACGTGCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATCCCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTaa                                                                                                                                                     <  2:112873/251‑1 (MQ=255)                                                                           gccGTTCTCCCTTAGGGCCGTTGCTGGCTCACCTTTATCCTTCCTGGTGCCTGCTGTTAATACCGCTATTTCTTCGATACCATCATTGCTGAAGGTTAAGGAGAATATGGTGCATCCGGTAGGATTCGACCCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATGCCGCTACTAAAGCCATATCAATTAAGAGATGGt                                                                                                                                             <  2:208284/249‑1 (MQ=17)                                                                                        cGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGggagg                                                                                                                                 <  2:263595/250‑1 (MQ=255)                                                                                                    ggCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTg                                                                                                                    >  1:26108/1‑251 (MQ=255)                                                                                                        cAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGc                                                                                                                >  1:201161/1‑251 (MQ=255)                                                                                                         aaCGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTAATTTTACTGCTGATATTGATACCGCTACTAAAGCCTAATCAATTAAGAGATGGTGAATCCGGGAGGATTAccagccttcgggcc                                                                                                               >  2:123848/1‑238 (MQ=17)                                                                                                           cGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGATCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATCTTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTAGCCTTGCGGCTcc                                                                                                             >  2:579053/1‑250 (MQ=255)                                                                                                              tATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGccc                                                                                                          >  1:186090/1‑251 (MQ=255)                                                                                                                    tcctGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCgg                                                                                                     <  2:186090/250‑1 (MQ=255)                                                                                                                          tGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTCTCGAGCTGAACTACGGATGCCTCGGGAACACTAATTCTACTCCTGATATGGATACCGCTACTAAAGCCATATCAATTAAGAGCTGGTGCAACCGGGCGGATTACTCGCCTGGCGGCTccgcctgcatgcggttg                                                                                              >  2:413820/1‑235 (MQ=17)                                                                                                                                               tctcTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATccc                                                                         >  1:251238/1‑251 (MQ=255)                                                                                                                                               tctcTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATcc                                                                          <  2:251238/250‑1 (MQ=255)                                                                                                                                                     cGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTg                                                                    <  2:374345/250‑1 (MQ=255)                                                                                                                                                             tCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGt                                                            <  1:151755/250‑1 (MQ=255)                                                                                                                                                             gcATTGCTGAAGGTTACGGGGAATATGGTGCTTCCGGGAGGATGCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAATTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCGACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGTATTACTCGCCTTGCGGCGCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGtt                                                           <  2:555093/250‑1 (MQ=18)                                                                                                                                                              cATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTa                                                          <  1:390278/251‑1 (MQ=255)                                                                                                                                                                            tcggatagtttGGTGTATACGGAAGGATTCGAACCACCGACCGCGCGGGTCGTAGACGAGTACTCTATCCAGCTAAACTAAGGATGCTGCGGAAAAATTTATTCTACTGCTGATAGTGAGACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTActtc                                             <  2:66435/240‑4 (MQ=17)                                                                                                                                                                               gagaATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCCCTTCg                                         >  1:173568/1‑251 (MQ=255)                                                                                                                                                                               gagaATATGGGGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCGGAGTACTCTATCCAGCTGAACTACGGATGCAACGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCg                                         <  2:407774/251‑1 (MQ=255)                                                                                                                                                                                   atatGGTGCCTCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGATTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATAc                                     <  1:318947/251‑1 (MQ=255)                                                                                                                                                                                          ggaTCCGGGAGGATGCGAACCTCCGACCGCTGGGTTCGTAGCCGAGTTCTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                              <  1:143107/249‑1 (MQ=255)                                                                                                                                                                                          gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAtt                              <  1:329107/251‑1 (MQ=255)                                                                                                                                                                                          gCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCAt                               <  1:212760/250‑1 (MQ=255)                                                                                                                                                                                                   aggaTTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAt                     <  1:353496/251‑1 (MQ=255)                                                                                                                                                                                                                    ccGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGc    <  1:579053/251‑1 (MQ=255)                                                                                                                                                                                                                      gCTCGGTTCGTAGCCGAGTACTCTATCCAGCGGAACTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAt  <  1:534103/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                         GAGTTATTTAGAAATGAGATATTTTTGAAGGAAATTTTTTGGAGAAGATGGTGCATCCGGGAGGATGACTCACTGACGTTCGCCCTTCGGGCCGTTGCTGGCGCAACGTTATCCTTCCTGGTGCCTGCGGTTAATACCGCTATCTCTTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAACCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAACTTATTCTACTGCTGATATTGATACCGCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCAT  >  NC_000913/2817603‑2818066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑