Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,780,060 G→A H889H (CAC→CAT)  ypjA ← adhesin‑like autotransporter YpjA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,780,0600GA91.7% 132.7 / 2.7 48H889H (CAC→CAT) ypjAadhesin‑like autotransporter YpjA
Reads supporting (aligned to +/- strand):  ref base G (3/1);  new base A (25/19);  total (28/20)
Fisher's exact test for biased strand distribution p-value = 6.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

CAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCT  >  NC_000913/2779819‑2780306
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
cAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTAGAGTCGCGAGCTTC‑GGTAAC‑TGCTAATACCCATAAATTACCGCCATTTTCCAGCAACATATTGGTTGCTAAATTGCCGGAAATGGCAAAAGTACCGTACTGATGAGTACCg                                                                                                                                                                                                                                               >  1:225746/1‑251 (MQ=255)
   cGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCCACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTg                                                                                                                                                                                                                                            >  1:356378/1‑251 (MQ=255)
    gcctgggggacttcccgccgggtccAGAGCTGGAAACTCATCGTTTGCCCCCCCTGGGGTATTTTC‑CCCGCCAGATTTACCCTTTGGGGGGTAGTCCTGCCCCGGGTTTTGTATGGCCCCACCCTTCCCAACCGTGGTGTCGCGTGTTGC‑GGTCCT‑TGCTAATACCAATAAATTGCCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATTGAAAAAGTACCGTACTGATGAGTACCGCTGa                                                                                                                                                                                                                                           <  2:568474/228‑1 (MQ=255)
                     cccGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCAAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGCCCCAGGTTTTGCATTGCCCCCCCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGcc                                                                                                                                                                                                                          <  1:427920/251‑1 (MQ=255)
                           ccAGAGCCTGAACCTCATCTTTTGCCCGCCCAAGGGTATATTG‑CCCGCCAGATTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTGGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAAAAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTc                                                                                                                                                                                                                    <  1:46375/251‑1 (MQ=255)
                                gcttGAAACTCACTTTTTGCCCCCCAAAGGGTATTTTG‑CCCGCCAGAGTTAACCTTTGGGGGGGGGTCCTGACCCAGGTTTTGAATTGCCCCCCCCTTGCCAACCGGGGAGGCGCGGGCTTC‑GGTCCC‑TGATAAAACCAAAAAATTACCGCCCATTTCCAGCCACCTAATGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAAAACCGTTAGCCGTGCTCGTCTg                                                                                                                                                                                                               <  2:214743/248‑1 (MQ=255)
                                        cTCATCTTTTGACCGCCCAAGGGTATATTG‑CCCGCCAGAGCTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGCGAGTACCGCTGATTTCAATACCGTTAGCCGGGCTCGTCTTGAGAGCgg                                                                                                                                                                                                       >  2:132763/1‑251 (MQ=255)
                                         tCATCTTTTGACCGCCCAAGGGGATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGCGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATCTTTGTCGCTAAATTGCCGGAAATTGAAAAAGTCCCGTACTGATTAGTACCGCTGCTTTCCATACCGTTAGCCGTCATCGTCTggggtggggc                                                                                                                                                                                                      >  2:253579/1‑243 (MQ=255)
                                                     ccccaaaGGGT‑TATTGGCCCGCAAGAGCTACCCTTGGTGGCGGGGTCCTGACCCGGGTTTTGCATTGCCCCCCCCGTGCAAACCGTGGAGTCGCGAGCTTC‑GGTCCC‑TGCTAATACCAAAAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTg                                                                                                                                                                                          <  2:395450/246‑1 (MQ=255)
                                                      ccccAAGTGTATATTG‑CCCGCCGTATTTACCTTTTGTGGCGGAGTCCTGACCAAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTgg                                                                                                                                                                                         <  1:124628/250‑1 (MQ=255)
                                                        ccAAGGGTATATTC‑CCCGCCAGAGTTACCCTTTGTGGCGGAGCCCTTACCAAGTTTTTGCATTCCCCCACCCTTCCCCACCGGGGATTCGCGAGCTCC‑GGTCCC‑TGCAAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGAc                                                                                                                                                                                       <  2:356378/251‑1 (MQ=255)
                                                         cAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCCACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACg                                                                                                                                                                                      >  2:282515/1‑251 (MQ=255)
                                                                   ttG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGAACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCg                                                                                                                                                                            >  1:273493/1‑251 (MQ=255)
                                                                            cAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGcc                                                                                                                                                                     >  1:294594/1‑250 (MQ=255)
                                                                                  tAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATc                                                                                                                                                               <  1:308532/250‑1 (MQ=255)
                                                                                             gCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCACCCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGCGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAg                                                                                                                                                   <  1:132763/251‑1 (MQ=255)
                                                                                                       gACCCGGGTTTTGCATGGCCCCCCCCTGCCCAACCGGGGTGTCGCGGGCTTC‑GGTACC‑TGCAAATACAAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCt                                                                                                                                         <  2:413693/251‑1 (MQ=255)
                                                                                                       gACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCt                                                                                                                                         >  1:128615/1‑251 (MQ=255)
                                                                                                                tttGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCCTTGATc                                                                                                                                >  1:353483/1‑251 (MQ=255)
                                                                                                                     aTTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATCTTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTCTCGTTTCCTGTCAGCGTACCGCCTTCATTTATCTTTGt                                                                                                                           >  2:136651/1‑251 (MQ=255)
                                                                                                                          cccaccccgcccAACCGTGGAGTCGGGA‑CGTCTGATACC‑TGTTAATCCCAAAAAATAACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTa                                                                                                                      <  2:39414/241‑1 (MQ=255)
                                                                                                                          cccACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCATACCGCCTTCATTGATCTTTGtttttt                                                                                                                      >  2:390586/1‑250 (MQ=255)
                                                                                                                            cACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTAtt                                                                                                                    >  2:298541/1‑251 (MQ=255)
                                                                                                                                 ttGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCctc                                                                                                               >  2:10549/1‑251 (MQ=255)
                                                                                                                                  tgcacaCCGTGGATCCGCGAGCTTC‑GGTACT‑TGCTAACACCAAAAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCct                                                                                                                <  2:351735/244‑1 (MQ=255)
                                                                                                                                         ggTGGAGTAGCGCGCTTC‑G‑TACCCTGCAAATACCAAAAAAGTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAg                                                                                                       <  2:216685/250‑1 (MQ=255)
                                                                                                                                         cGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAA‑GTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGc                                                                                                      >  1:16433/1‑251 (MQ=255)
                                                                                                                                                                      tAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCcgtcgt                                                                            >  2:173592/1‑251 (MQ=255)
                                                                                                                                                                                 aaTTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCACTACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCCTTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGtc                                                                 >  2:166025/1‑251 (MQ=255)
                                                                                                                                                                                  aTTACCGCCATTTTCCAGCTACATATTGGTCGCTAAATTGCCGGAAATGGCAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGCCGATATCTGTCGCTTTGCCATTATCGTTAACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTACGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATtgttgtgt                                                                >  2:364433/1‑249 (MQ=255)
                                                                                                                                                                                    tACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGtctc                                                               >  1:540219/1‑250 (MQ=255)
                                                                                                                                                                                           aTTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCCTTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGccc                                                       >  2:493747/1‑250 (MQ=255)
                                                                                                                                                                                                 cAGCAACAGATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGGACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTGCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCt                                                 <  2:534938/251‑1 (MQ=255)
                                                                                                                                                                                                          atTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAAtt                                         <  1:298541/250‑1 (MQ=255)
                                                                                                                                                                                                            tGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGt                                      >  1:231368/1‑251 (MQ=255)
                                                                                                                                                                                                                   tAAATTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCAccgc                               >  1:260171/1‑251 (MQ=255)
                                                                                                                                                                                                                    aaaTTGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCAccg                                <  2:294594/249‑1 (MQ=255)
                                                                                                                                                                                                                        tGCCGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTg                          >  1:473741/1‑251 (MQ=255)
                                                                                                                                                                                                                           cGGAAATGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGc                       <  1:282515/251‑1 (MQ=255)
                                                                                                                                                                                                                            ggAAATGTAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGGGCTCGTCTGGAGAGCGGCCCCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTCCCGCTTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCTTTAATTGTGTTCTCATCCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGcg                      <  1:253579/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaTGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGcgtcg                   >  2:201296/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACACTTTGTTCACCGCCGGTGAGCGTCGTg                 >  1:577846/1‑251 (MQ=255)
                                                                                                                                                                                                                                 tGGAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTg                 >  2:375956/1‑251 (MQ=255)
                                                                                                                                                                                                                                   gAAAAAGTACCGTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGcc               >  2:54992/1‑251 (MQ=255)
                                                                                                                                                                                                                                    aaaaaGTACCGTACTGATGAGTACCGCTGATTTCAATCCCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCTTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAACCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCCCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCt              <  1:173592/251‑1 (MQ=255)
                                                                                                                                                                                                                                    aaaaaGTACCGTACTGAGGAGTACCGCTGATTTCAATCCCGTTAGCCGTGCTCGTCTGGAGAGCGGCCCCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCCCCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCt              <  1:390586/251‑1 (MQ=255)
                                                                                                                                                                                                                                              gTACTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACAGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTAAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGAGCCTGTCGCCGTgg    >  1:538310/1‑251 (MQ=255)
                                                                                                                                                                                                                                                aCTGATGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGCGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCt  >  1:41553/1‑251 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      
CAGCGACCTGGAGATCTTCTGCCCGGGCCAGAGCCTGAAACTCATCTTTTGACCGCCCAAGGGTATATTG‑CCCGCCAGAGTTAACCTTTGTGGCGGAGTCCTGACCCAGGTTTTGCATTGCCCCACCCTTGCCAACCGTGGAGTCGCGAGCTTC‑GGTACC‑TGCTAATACCAATAAATTACCGCCATTTTCCAGCAACATATTGGTCGCTAAATTGCCGGAAATGGAAAAAGTACCGTACTGGTGAGTACCGCTGATTTCAATACCGTTAGCCGTGCTCGTCTGGAGAGCGGCACCGCTGTTCTGGACGATATCTGTCGCTTTGCCATTATCGTTGACTGTCAGCGTACCGCCTTCATTGATCTTTGTTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCT  >  NC_000913/2779819‑2780306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑