Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,748,396	G→A	A145A (GCG→GCA)	ypjD →	cytochrome c assembly family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,748,396	0	G	A	94.4%	161.5 / 0.6	55	A145A (GCG→GCA)	ypjD	cytochrome c assembly family protein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base A (29/22);  total (31/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.52e-01

AGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACAT  >  NC_000913/2748151‑2748642                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       aGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCCCCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGcc                                                                                                                                                                                                                                                   >  2:159977/1‑250 (MQ=255)   ccTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGCTGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTCTCGCCGCCCTGTCCGCACTGccac                                                                                                                                                                                                                                                 >  2:148632/1‑248 (MQ=255)        tgAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGACTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCCACCTTCATGCCCAATGCATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CCCATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCg                                                                                                                                                                                                                                            >  2:539164/1‑251 (MQ=255)         gAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGc                                                                                                                                                                                                                                             <  1:154983/249‑1 (MQ=255)                   tCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCCCCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTCTGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGACGTGgattgattc                                                                                                                                                                                                                                 >  1:18160/1‑250 (MQ=255)                   tCATTGGGCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTTTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCTCCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGgattgatt                                                                                                                                                                                                                                  <  2:364505/250‑1 (MQ=255)                    cATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTa                                                                                                                                                                                                                                 <  2:455553/250‑1 (MQ=255)                         gTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACt                                                                                                                                                                                                                           <  2:530379/251‑1 (MQ=255)                           cAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTga                                                                                                                                                                                                                         <  1:576885/251‑1 (MQ=255)                            aGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTgaa                                                                                                                                                                                                                        <  1:239494/251‑1 (MQ=255)                                 gatgatCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAAc                                                                                                                                                                                                                    <  2:138685/250‑1 (MQ=255)                                     atCTGTACGGTAATGACCATTGTGGCTTCTCGCACTCGTGGCTGGATGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTAAGCACTTCAACTGGCGTGGCTTGATTCCAAACTGAAGAACaaca                                                                                                                                                                                                                >  2:201387/1‑248 (MQ=255)                                     atCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACaaga                                                                                                                                                                                                                >  1:291615/1‑250 (MQ=255)                                           aCGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCTCCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTgg                                                                                                                                                                                                          <  1:114277/250‑1 (MQ=255)                                           aCGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGc                                                                                                                                                                                                         >  1:422322/1‑251 (MQ=255)                                            cGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCg                                                                                                                                                                                                        >  2:233191/1‑251 (MQ=255)                                              gTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGtt                                                                                                                                                                                                      <  1:573022/251‑1 (MQ=255)                                              gTAATGACCAGTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGtt                                                                                                                                                                                                      <  2:85481/251‑1 (MQ=255)                                                   gtccagtgtgGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACAGCACCCATCTGGAAGCTACGCCTTGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTGCGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAAc                                                                                                                                                                                                  <  1:557184/244‑1 (MQ=255)                                                    aCCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTCTCAACCTGGCGCTGGCAACCTTCATGCCCACTGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACCGAAGAACAAGAAGCTGGCGTTTAAcc                                                                                                                                                                                                 >  2:387410/1‑250 (MQ=255)                                                          gtgGCTTCTAGCAATCGTGGCTGGCTGCTGCTACCCATTTTCTCTGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCGGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCCCCGCCCTGTACGCACTGCAACTGTCGTGTATTGATTACCCACTGAAAAACAAGAAGCGGGCGTTTATCCAGGaaa                                                                                                                                                                                           >  2:104610/1‑250 (MQ=255)                                                            ggCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACACGAAGCTGGCGTTTAACCAGGAAATgc                                                                                                                                                                                        >  1:64642/1‑251 (MQ=255)                                                                    gCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTACTTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATtg                                                                                                                                                                                >  2:123422/1‑251 (MQ=255)                                                                          gTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGt                                                                                                                                                                          <  1:385890/251‑1 (MQ=255)                                                                            ggctggctGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTAt                                                                                                                                                                        >  2:240849/1‑251 (MQ=255)                                                                             gctggctgCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATc                                                                                                                                                                       >  1:340510/1‑251 (MQ=255)                                                                                 gctgctgctACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGc                                                                                                                                                                   >  2:520881/1‑251 (MQ=255)                                                                                   tgctgctACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGACGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGt                                                                                                                                                                 >  1:210165/1‑251 (MQ=255)                                                                                             cATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTcc                                                                                                                                                       <  1:65876/251‑1 (MQ=255)                                                                                                 gTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACAt                                                                                                                                                   >  1:481552/1‑251 (MQ=255)                                                                                                        ccTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAg                                                                                                                                            <  2:199866/251‑1 (MQ=255)                                                                                                         cTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGACGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGc                                                                                                                                              >  2:497192/1‑248 (MQ=255)                                                                                                              cgcTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGc                                                                                                                                      <  1:123422/251‑1 (MQ=255)                                                                                                                      aaCCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTCCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGtgct                                                                                                                              <  2:233321/251‑1 (MQ=255)                                                                                                                                                     aaTACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTc                                                                                               >  1:318476/1‑251 (MQ=255)                                                                                                                                                       tACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCtc                                                                                             >  1:499796/1‑250 (MQ=255)                                                                                                                                                       tACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCt                                                                                              >  2:482504/1‑250 (MQ=255)                                                                                                                                                                    tGGAAGCTACGCCTGGGATGCTGGAG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGGTTGGCGTGGTGCTGCTCACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACACCtt                                                                                 >  2:437788/1‑250 (MQ=255)                                                                                                                                                                        aGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGGGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTAttt                                                                             >  2:209972/1‑250 (MQ=255)                                                                                                                                                                                cTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCCTGGa                                                                     >  2:442397/1‑250 (MQ=255)                                                                                                                                                                                  gggATGCTG‑TGTCACATTGGCTTACCGGTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTTCCAATTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGTCATTGATGAGTATCGATCGTAAAATGTTCCACATCACGCAGATTTGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCTCAACTTATTTAGCATGGaaaa                                                                  <  2:391216/251‑1 (MQ=255)                                                                                                                                                                                  gagATGCTGGCG‑CCCATCGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATGGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCCACTGAAGCACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGGTGAGTATCGAGCGTAAAATGTTCCACATCACGCAGTGTGGAGTGGTGCTGCTACCGCTTAAGCTTTGCACTGGCCTGTTCTCGATGCACAACTTATTTAGCATggcaac                                                                  >  2:245217/3‑247 (MQ=255)                                                                                                                                                                                             g‑CACAATGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAg                                                        <  2:122110/250‑1 (MQ=255)                                                                                                                                                                                                acaTTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCt                                                     <  1:433859/251‑1 (MQ=255)                                                                                                                                                                                                acaTTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCt                                                     >  1:108190/1‑251 (MQ=255)                                                                                                                                                                                                                ctTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtgg                                     >  1:387523/1‑251 (MQ=255)                                                                                                                                                                                                                  tttCCTATGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATcgtg                                      <  1:295435/248‑1 (MQ=255)                                                                                                                                                                                                                         tGCCACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTg                            >  1:176782/1‑251 (MQ=255)                                                                                                                                                                                                                           ccACGCTAATTATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGCAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTc                          >  2:192540/1‑251 (MQ=255)                                                                                                                                                                                                                               gttAATTATCGCCGCCCTGTCCGCCCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCtata                      <  2:181685/249‑1 (MQ=255)                                                                                                                                                                                                                                    ttATCGCCGCCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACTAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCATAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATtgtg                 >  2:74620/1‑251 (MQ=255)                                                                                                                                                                                                                                        cgccgcCCTGTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGtgctgc             >  1:137700/1‑251 (MQ=255)                                                                                                                                                                                                                                         cccgccCTGTACGCACTGCAACGGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTATATATTGTgctgct            <  2:499796/250‑1 (MQ=255)                                                                                                                                                                                                                                                 gTACGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGAc    <  1:466266/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aCGCACTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACAt  <  1:159977/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       AGCCTGCTGAACGTTGGTTCATTGGTCAGTTTGATGATCTGTACGGTAATGACCATTGTGGCTTCTCGCAATCGTGGCTGGCTGCTGCTACCCATTGTCTATGCCTTTGCGCTTATCAACCTGGCGCTGGCAACCTTCATGCCCAATGAATACATCACCCATCTGGAAGCTACGCCTGGGATGCTGGTG‑CACATTGGCTTATCGCTCTTTTCCTATGCCACGCTAATTATCGCCGCCCTGTACGCGCTGCAACTGGCGTGGATTGATTACCAACTGAAGAACAAGAAGCTGGCGTTTAACCAGGAAATGCCGCCATTGATGAGTATCGAGCGTAAAATGTTCCACATCACGCAGATTGGCGTGGTGCTGCTAACGCTCACGCTTTGCACTGGCCTGTTCTACATGCACAACTTATTTAGCATGGAAAATATCGACAAGGCTGTGCTCTCTATCGTGGCGTGGTTTGTCTATATTGTGCTGCTGTGGGGACAT  >  NC_000913/2748151‑2748642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑