Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,700,747	G→A	L84L (CTC→CTT)	acpS ←	holo‑[acyl‑carrier‑protein] synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,700,747	0	G	A	97.9%	165.4 / ‑4.0	47	L84L (CTC→CTT)	acpS	holo‑[acyl‑carrier‑protein] synthase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (26/20);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

ACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATT  >  NC_000913/2700500‑2700993                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       acTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGc                                                                                                                                                                                                                                                     >  1:410891/1‑251 (MQ=255)    gATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCATCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCtca                                                                                                                                                                                                                                                  <  1:343146/251‑1 (MQ=255)     aTCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCtcat                                                                                                                                                                                                                                                 >  1:551860/1‑251 (MQ=255)         aTAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCAtt                                                                                                                                                                                                                                             <  2:423085/251‑1 (MQ=255)            aTCGCTGTTGTTGAGGGCTCATGCTGTACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTCACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAAGTACCGTGGCACAAGCATAGGGCCGCTCAGCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTCTTAATGCCTCGCCCCATAGCCGTCGCCGTTGTTTGCCAAGCTCATCATTGaa                                                                                                                                                                                                                                          <  2:300481/251‑1 (MQ=255)              cGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAAt                                                                                                                                                                                                                                         >  1:406797/1‑250 (MQ=255)                        gAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTg                                                                                                                                                                                                                              <  2:414153/251‑1 (MQ=255)                                        caAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGAcc                                                                                                                                                                                                               >  1:160688/1‑250 (MQ=255)                                          aaaTTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCAtt                                                                                                                                                                                                            <  1:57695/251‑1 (MQ=255)                                              tAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCg                                                                                                                                                                                                         <  2:406797/250‑1 (MQ=255)                                                            aaaaCCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTTCGAATCCCGGTGCCaa                                                                                                                                                                                           >  2:493124/1‑250 (MQ=255)                                                            aaaaCCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGATTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATACCGGTGCCaa                                                                                                                                                                                           >  2:213139/1‑250 (MQ=255)                                                              aaCCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAAcc                                                                                                                                                                                        >  2:29343/1‑250 (MQ=255)                                                                                    gggggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCa                                                                                                                                                                                            <  1:273619/225‑1 (MQ=255)                                                                                    gggggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCa                                                                                                                                                                                            >  2:273619/1‑225 (MQ=255)                                                                                     ggggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCt                                                                                                                                                                                      <  2:377023/230‑1 (MQ=255)                                                                                     ggggTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCt                                                                                                                                                                                      >  1:377023/1‑230 (MQ=255)                                                                                            tACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGGGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCaaaa                                                                                                                                                           <  2:34798/250‑1 (MQ=255)                                                                                                           aCGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAAc                                                                                                                                           >  2:212600/1‑251 (MQ=255)                                                                                                             ggTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCCGAAACCGc                                                                                                                                         >  2:271684/1‑251 (MQ=255)                                                                                                             ggTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGc                                                                                                                                         >  1:458290/1‑251 (MQ=255)                                                                                                                attattTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCAcc                                                                                                                                      >  1:453225/1‑251 (MQ=255)                                                                                                                           cTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGggggtg                                                                                                                           >  2:350126/1‑251 (MQ=255)                                                                                                                                           tGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGcc                                                                                                           >  1:211766/1‑251 (MQ=255)                                                                                                                                             gCACAAGCATAGTGCCGCTCATCTGCCATCGTTACATGCATATTTGCATCGCCCAGCGTTCCCGCCAGTTTTAATGCCTCGCCCCAGAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCTGCTTCTTACACAGCAAAACTCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCa                                                                                                         <  1:544165/251‑1 (MQ=255)                                                                                                                                               acaAGCATAGTGACGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCAtt                                                                                                       >  1:128773/1‑251 (MQ=255)                                                                                                                                                     aTAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGCGGGGCGTTTTCCAGATAGCCCATTccttct                                                                                                 >  2:106999/1‑246 (MQ=255)                                                                                                                                                       aGTGCCGCTCCTCTGCCAGCGTTACATGCATAGTTGCAACGCTCAGCTTTTCCGCCAGGTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTTCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCTCATCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATc                                                                                                <  2:501568/250‑1 (MQ=255)                                                                                                                                                                        aGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGc                                                                              >  2:356363/1‑251 (MQ=255)                                                                                                                                                                             tACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAgg                                                                          <  1:475354/250‑1 (MQ=255)                                                                                                                                                                                aTGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGAt                                                                      <  2:206074/251‑1 (MQ=255)                                                                                                                                                                                       tttGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGGGGGGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCgg                                                                >  1:357424/1‑250 (MQ=255)                                                                                                                                                                                          gCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATc                                                             <  1:212600/250‑1 (MQ=255)                                                                                                                                                                                                      ttttCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGc                                                >  1:309266/1‑251 (MQ=255)                                                                                                                                                                                                         tCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTACACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGGGCGTTTTCCAGATAGCCCATTCGTTATCGACTAATACGCTGAGTGCCAGGCGATCACCGGATCGGGCGAGCACCGCTTc                                             >  2:101747/1‑251 (MQ=255)                                                                                                                                                                                                                gTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCCCCGGATCGGGCGATCACCGCTTCGAttcg                                       >  2:449793/1‑247 (MQ=255)                                                                                                                                                                                                                   ttAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCCTTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgggcg                                   >  2:2111/1‑251 (MQ=255)                                                                                                                                                                                                                   ttAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagc                                    <  1:375850/250‑1 (MQ=255)                                                                                                                                                                                                                    tAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATgcgagcg                                   <  1:2111/250‑1 (MQ=255)                                                                                                                                                                                                                     aaTGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAt                                 >  2:17019/1‑251 (MQ=255)                                                                                                                                                                                                                      aTGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGAtc                                <  2:410891/251‑1 (MQ=255)                                                                                                                                                                                                                             gCCCCATAGCCGTAGCCGTGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACaa                         <  1:155639/251‑1 (MQ=255)                                                                                                                                                                                                                                        gtagccgtGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCt              >  1:330022/1‑251 (MQ=255)                                                                                                                                                                                                                                          agccgtGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTa             <  1:29343/250‑1 (MQ=255)                                                                                                                                                                                                                                              gtGGTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAACACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATCGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCCCCGGATCGGGCGATCACCGCTTCGATGCGAGGTATCCCCCCAATCTCCGTGCCTACAccc        >  2:4807/1‑250 (MQ=255)                                                                                                                                                                                                                                                ggTTTGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTa       <  1:356363/250‑1 (MQ=255)                                                                                                                                                                                                                                                    tGCCAAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGGGCCTAAACCTAATAtt  >  2:290057/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       ACTGATCCATAATCGCTGTTGTTGAGGGTGCATGCTGCACAAAATTAAAGTTAAAAAGTAAAACCCCCGTTCCTTACCAGTTCGGGGGTTTTACTTTTTAAAGAGAACGGTATTATTTTTAACTTTCAATAATTACCGTGGCACAAGCATAGTGCCGCTCATCTGCCAGCGTTACATGCATATTTGCAACGCCCAGCTTTTCCGCCAGTTTTAATGCCTCGCCCCATAGCCGTAGCCGTGGTTTGCCGAGCTCATCATTGAATACTTCAAATTGATTAAACGCCAGACCATTGCGGATCCCGGTGCCAAACGCTTTTGCTGCGGCTTCTTTCACAGCAAAACGCTTCGCCAGAAAACGCACCGGCTGGTGGTGCGTTTTCCAGATAGCCCATTCGTTATCGCTTAATACGCGGCGTGCCAGGCGATCACCGGATCGGGCGATCACCGCTTCGATGCGAGCGATCTCCACAATATCCGTGCCTAAACCTAATATT  >  NC_000913/2700500‑2700993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑