Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,614,321	2 bp→GT	intergenic (+61/+358)	yjjV → / ← yjjW	putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,614,321	0	A	G	95.5%	41.8 / NA	22	intergenic (+61/+359)	yjjV/yjjW	putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (14/7);  minor base T (0/1);  total (14/8)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01
*	NC_000913	4,614,322	0	A	T	100.0%	48.7 / NA	22	intergenic (+62/+358)	yjjV/yjjW	putative metal‑dependent hydrolase YjjV/putative glycyl‑radical enzyme activating enzyme YjjW
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (14/8);  total (14/8)

GCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTA‑CAGGT‑TTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614078‑4614470                                                                                                                                                                                                                                                    ||                                                                                                                                                       gCATCGTTGTTTCTGGAACCCGAAGCGCCGGATATGCCGTTCTACGGTTTGAAGGGGAAGCCCACCCGCCCGGGGCAGGCTGCCCGTGTGTCCGCCGTGCTTTGCGAGTTGCGCCGGGAGCCGGCGGATGAGAGTTCGCAAGCGCTGCTTAATAACACGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGTCGTTCACGCCGCATCCGGCAGTTGGCGATCAATGCCTGATGCGACGCTTGTCGCGTc                                                                                                                                                  <  2:234852/251‑1 (MQ=255)            gcgggAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCTTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGTATAAGGCGTTCACGCCTCATCCGGCAGGTGGCTCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGcc                                                                                                                                        <  1:260647/247‑1 (MQ=38)             cTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGCGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCCGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATTCCt                                                                                                                                       >  2:5606/1‑250 (MQ=255)                                  atGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGt                                                                                                                 >  1:76310/1‑249 (MQ=255)                                    gCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGTCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTGTAACGTGCCGTAGGCCGGATAAGGCGGTCACGCCGCATCCGGCAGTTGGCGCACAATGCCGGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAgg                                                                                                              <  1:118731/250‑1 (MQ=37)                                     ccGCTCAACGGTTTTCAGGGGAAGCCTACCCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGCTGCACCGGGAACCGGCGGATGAGAATGCGCAACCGTTGCTTAATAACCCGTATACGTTGTTTAACGTGCCGTAGGACGGATAACGCGTTCACGCCGCCTCCGGCCGTAGGCGCACAATGCCTGATGCGACACTTGTCGAGGCTTATCATGACTAACAG‑C‑CTGTGCCGAACCGTAGGcc                                                                                                            >  2:267451/1‑251 (MQ=255)                                      cGCTCACCGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGCTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTTCCGTAAGCCGGATAAGGCGATCACGCAGCATCCGGCCGTTCGCGCACAATGCCTGATGCGACGCTTGTCGCATCTTATCATGCCTA‑CACGTCTTGT‑CCGAACCGTaacccc                                                                                                           >  2:272141/1‑246 (MQ=255)                                                                aaCCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGGCTTATCATGCCTA‑CAAGT‑CTGTGCCGAAACGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGc                                                                                 >  2:3025/1‑251 (MQ=25)                                                                      ccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGCGTTTCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGAAAAGGCGTTCACACCGCATCCGGCAGTTGGCGCCCCATGCCTGATGCGACGCTTGTCGCTTCTTATCATGCCTA‑CAAGT‑CTGTGCCGCACCGTAGGCCGGCTAAGGCATTccacacacatccgtcagccgg                                                                           >  2:271614/1‑231 (MQ=255)                                                                      ccGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTTTCGCGTCTTATCATGCCTA‑TAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                     <  1:303237/241‑1 (MQ=255)                                                                      ccGGAGCAGGCTACCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTACTAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTTGGACGGAACAGGCGTTCACGCCGCATc                                                                                     >  2:303237/1‑241 (MQ=21)                                                                          ggCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTGGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACCCGTATACGTTGTTTAACGGGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGca                                                                       <  1:3025/250‑1 (MQ=18)                                                                             gggCTGCCGGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGcataa                                                                    <  1:85209/250‑4 (MQ=18)                                                                                cTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGc                                                                 >  1:86459/1‑251 (MQ=18)                                                                                                cGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGAAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGATTGTCGAGTCTTATAATGCCTA‑CAAGT‑CTGTGCCGAAACGAAGGCAGGATAAGGCGTTCACGCCGCATCCCGAAGTCGGCGCATAATGCCTGATGCGACGCTTGt                                                 >  1:186872/1‑251 (MQ=17)                                                                                                 gTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTc                                                >  1:240063/1‑251 (MQ=255)                                                                                                  tGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcg                                               >  1:41747/1‑251 (MQ=255)                                                                                                   gCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTcg                                               <  1:5606/250‑1 (MQ=255)                                                                                                                         ccGGCGGATTAGATTGCGCACGCGTTGCTTAATAACTCGTAGACGTTGTTGAACGTGCCGGAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTAGCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACaggttt                        <  1:286458/251‑6 (MQ=11)                                                                                                                                aTGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCg                  >  1:178013/1‑250 (MQ=11)                                                                                                                                        gcgcAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAgg         >  1:287865/1‑251 (MQ=11)                                                                                                                                                 ttGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGAAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTA‑CAAGT‑CTGTGCCGCACCGTAGGCCGGATAAGGCGTTCACGCCGCCTCCGGCAGTCGGCGCATAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAGGTTTGTGCCGAACCGTAGGCCGGATa  >  1:277762/1‑249 (MQ=11)                                                                                                                                                                                                                                                    ||                                                                                                                                                       GCATCGTTATTGCTGGAAACCGACGCGCCGGATATGCCGCTCAACGGTTTTCAGGGGCAGCCTAACCGCCCGGAGCAGGCTGCCCGTGTGTTCGCCGTGCTTTGCGAGTTGCGCCGGGAACCGGCGGATGAGATTGCGCAAGCGTTGCTTAATAACACGTATACGTTGTTTAACGTGCCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTAACGCGTCTTATCATGCCTA‑CAGGT‑TTGTGCCGAACCGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTTGGCGCACAATGCCTGATGCGACGCTTGTCGCGTCTTATCATGCCTACAAGTCTGTGCCGAACCGTAGGCCGGATA  >  NC_000913/4614078‑4614470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑