| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 480,295 | 2 bp→AA | coding (13‑14/219 nt) | hha ← | hemolysin expression‑modulating protein Hha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 480,295 | 0 | G | A | 94.4% | 58.0 / NA | 18 | P5F (CCT→TTT) | hha | hemolysin expression‑modulating protein Hha |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/8); minor base C (1/0); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 480,296 | 0 | G | A | 100.0% | 59.6 / NA | 18 | P5F (CCT→TTT) | hha | hemolysin expression‑modulating protein Hha |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/8); total (10/8) | |||||||||||
ATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATA > NC_000913/480066‑480513 || ataGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCGCCAGCGTGTCAATTTTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTAc < 1:202618/251‑1 (MQ=255) ggAAGGGATCTTGTCGTACATTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTtc < 1:9286/251‑1 (MQ=255) ggATCTTGTCGTACAGTTTATTAATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGc > 1:239167/1‑251 (MQ=255) aTCTTGTCGTACAGTTTATTCATGGACAATTCGGCGAGGCGGTGATCTGCGGCTGCGTAAAATACCGCCAGTTCATTATCTGATAATTCGCATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTTGCAACGACGTAAACGCATTAAATAATCGGATTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTTTAACAAAATAAAATAGCAAGATTCTCTTTCGTCGcct > 2:259989/1‑249 (MQ=255) gtacattgtATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATCCCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTGCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACaaa < 1:200235/242‑1 (MQ=255) gTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGt < 2:54622/190‑1 (MQ=255) gTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGt > 1:54622/1‑190 (MQ=255) ttATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTATCGATAACCAGCTCCAGCGTGTCAATTGTCTTGCAAAGAAGTAAGCACATTAAATAATCGGATTTCGTTAAACATTTTTCGGACATACTTCTACCTATTGTTGTAATAATTCTATCAAGATAAAATCGAAGGGTTCTCTTTCGTCGCATTGACAAaacaaagg > 2:85567/1‑247 (MQ=255) aaaaTACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATACTCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATATTTCTAACAAGATAAACTCGCAGGCTTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGCCTTCCGCCATTTCTGAAGATCCTGCat > 2:57249/1‑251 (MQ=255) aTCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAATCGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTATCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTg > 1:261790/1‑251 (MQ=255) tttatttTTCTCGATAACACGCTAAAGCGTGTCAATTGTCAGGCAACGACGTAAACGCATTAAATACTCGGTTTTCGTTAAAAATTTTTCGGCCATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGgtg > 2:30482/1‑251 (MQ=255) gATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGatat > 1:27143/1‑251 (MQ=255) cGTGTCAATTGTCTGGCAACGACGTAATCGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTGTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCGGACTTCCGCCATTTCTGAAGAGCCTGCATATTAATACCATAACTACTGAACAACATAAATGTGTCATCCAGATAGTCGTCGATCTGCTCa < 1:119677/251‑1 (MQ=255) acgatgctctttcgatctAAACGCATTAAATAATCGGTTTTCTTTAAAAATTGTTCGGACATTCTTCTTCCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTTGTTCGTCGCCTTGACAAAACAACGGAATAGTCGATTACCTGACGTCCGCCATTTCTGAAGATCCTTCATATTAATACCATAACTACTGAACAACATAAAGGTGTCGTCCAGATATTCGTCGATCTGCTCAATGAGCt < 2:234647/234‑1 (MQ=255) gTAAACGCATTAAATAATCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATAGTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATtata < 1:116528/251‑1 (MQ=255) aaaCGCATTAAATACTCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATACTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCt > 1:234647/1‑233 (MQ=255) taatCGGTTTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAactac < 1:25723/170‑1 (MQ=255) taatCGGATTTCGTTAAAAATTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAAATCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAactac > 2:25723/1‑170 (MQ=255) || ATAGCTCCGAAAGCGAATTATTTATTAGCGAATAAATTTCCATACTGAGGAAGGGATCTTGTCGTACAGTTTATTCATGGTCAATTCGGCGAGGCGGTGATCTGCGGCTGAGTAAAATACCGCCAGTTCATTATCTGATAATTCGTATTTATTTTTCTCGATAACACGCTCCAGCGTGTCAATTGTCTGGCAACGACGTAAACGCATTAAATAATCGGTTTTCGTTAAAGGTTTTTCGGACATACTTCTACCTATGGTTGTAATAATTCTAACAAGATAAACTCGCAGGATTCTCTTTCGTCGCATTGACAAAACAACGGAATAGTCGATTACCTGACTTCCGCCATTTCTGAAGATCCTGCATATTAATACCATAACTACTGAACAACATAAAGGTGTCATCCAGATATTCGTCGATCTGCTCAATGAGCTTATTGTCTTCATTATA > NC_000913/480066‑480513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |