| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,834,224 | C→G | L105V (CTG→GTG) | norW → | NADH:flavorubredoxin reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,834,224 | 0 | C | G | 97.1% | 110.8 / ‑5.7 | 34 | L105V (CTG→GTG) | norW | NADH:flavorubredoxin reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (19/14); minor base T (0/1); total (19/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGCCAGTATTCTGGCGTCGTTAATGCCACCGG > NC_000913/2833981‑2834461 | cAGGAAGCCACTATTCCATTAACCCGGATTGCCGACGACAGAATGGAGGAGTCCAACCAACACGCCCCCAGCCATGTTATCAGTAAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCAGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCGGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGAAATACGACAAGCTAGTAGTGGCacc < 2:274790/251‑3 (MQ=255) cTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGAAGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGt > 1:271373/1‑251 (MQ=255) aTTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGTGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTTGCAAGACGACAAGCTAGTAGTGGCAACCGGTGCCAGt < 1:303931/249‑1 (MQ=255) aTTCCATTAACCCTGATTGCCGCCGACAGCATGGAGGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGc < 2:27910/251‑1 (MQ=255) ccgACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTggg < 1:173461/251‑1 (MQ=255) aGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTgg > 1:201364/1‑250 (MQ=255) aGCATGGATGAGTACAACAAACCGGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGCCCTTCCCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGa < 1:2354/251‑1 (MQ=255) aTGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGCAGCCCGTGTGGTGAAAAGCCAGAATAATAAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGAGTg > 2:294956/1‑247 (MQ=255) aTGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGtt > 1:22285/1‑251 (MQ=255) gggaggatgacaacaATCCTGCCCTCCGCCCTGCTAGCAGTCGGGGGCAACGGGCCGATGACCTTCCCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCCCCGGTTTCCACAAACCGGGGTGACTGATACCGATGCCGAATCCCTTGTGGTGAAAATCCTGAATAAGCAGTGGCATTCCGACAATCTAGTAGTGGCTACCGGGGCCAGTGCCTTTGTCCCGCCTTTGCCTGTGCGTGAGTTa < 2:135317/242‑1 (MQ=255) gATGAGTAAAACAACCCTGCCCCCGTCCATGTAATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAAt < 2:226656/251‑1 (MQ=255) aacaaACCTGAACTCAGCAATGTTATCAGTCAGGGGCACCGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGACTAATCAGGGGCAATACGACACGCTAGTAGAGGCAACCGGTGCCCGAGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGtt > 2:280717/1‑251 (MQ=255) tGTTATCAGTCAGGGGCAAAGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAACTTGCCGAGCAGTTTACTCTGCACCTGTTTCCACAAACCTGGGTGACGGCTATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTCGTGGCAACCGGTCCCAGAGCCTTTGTCCCGCCTGTGCCGGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGCATc > 2:223558/1‑251 (MQ=255) cccaacctcccTTCCCCGCCAGACGGCGGTGGAATTTGCCGAGCAGTTTAATCTGCCCCTGTTTCCACAAACCTGGGTGACGGATATCGAGGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGCCAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGc < 2:178716/242‑1 (MQ=255) aTGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGCGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGAt > 2:111247/1‑251 (MQ=255) aCCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGc > 1:178716/1‑243 (MQ=255) aCCCGCAAGACGGCGGTTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTCCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCATCCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTGAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGAc < 2:128109/250‑1 (MQ=255) cccGCCAGACGGCGGGTGAATTTGCCTACCCGTTTACTCTTCACCTGTTTCCACACACCCGGCTGACGGATATCGATGCAGGAGCCCGTGTAGTGCAACGACAGAATCATCAGTGGCAATACGCCAAGCTAGTAGCGGAACCCGGTGCCAGTGCGTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCGGTGAAACGCAACCGCGGGATGCCAGAcgc > 2:186650/1‑251 (MQ=255) gggTGAATTTGCCGAGCAGTTTAATCTGCACATGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGCttgttg > 1:269745/1‑251 (MQ=255) cAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTgg > 1:276963/1‑251 (MQ=255) gTTTCCACAAACCTGGGTGACGGATATCGATGCCGCAGCCCGTGTGGTGAAAAGCCAGAATATTCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGCAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGACCTGGCGATgg > 2:25176/1‑251 (MQ=255) tGACGGATATCGATGCCGCAGCCCGCGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACCAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGGTGGATTTTTGTCGTGAAggg > 2:203572/1‑250 (MQ=255) aCGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGTTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCaa < 1:16368/251‑1 (MQ=255) gATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGTCAAAGCGGTCAc > 2:69778/1‑251 (MQ=255) aaGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGAt < 1:276591/214‑1 (MQ=255) aaGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGAt > 2:276591/1‑214 (MQ=255) gCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCtgt < 2:225925/141‑1 (MQ=255) gCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCtgt > 1:225925/1‑141 (MQ=255) ggAAAAGCCAGAATAACCAGGTGCAATACGCCAAGCTAGAATTGGCAACCGGTGCCTGTGCCTGTGTCCCGCCTGTGCCTTGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCTACTGCGGGATGCCCGGCGCGTGTTGATTGTTGGCTGTGGTTTGATTGGTAGCGAACTGGCGATGGAGTTGTGTCGTGCAGGCAAAGCGGTCACGTTAATCGACAACGCtttc < 2:201787/250‑4 (MQ=255) aaaaGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCgg > 2:253945/1‑173 (MQ=255) aaaaGCCAGAATAATCAGTGGCAATACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCgg < 1:253945/173‑1 (MQ=255) aaTACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAAc > 2:266917/1‑221 (MQ=255) aaTACGACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGCCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAAc < 1:266917/221‑1 (MQ=255) gACAAGCTAGTAGTGGCAACCGGTGCCAGTGCCTTTGTCCAGACTGTGCCTGGGAGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGACTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGACAGTATTCTGGCGTCGTTAATGCCACCgg > 1:140411/1‑250 (MQ=255) | CAGGACGCCACTATTCCATTAACCCTGATTGCCGCCGACAGCATGGATGAGTACAACAAACCTGACCTCAGCCATGTTATCAGTCAGGGGCAACGTGCCGATGACCTTACCCGCCAGACGGCGGGTGAATTTGCCGAGCAGTTTAATCTGCACCTGTTTCCACAAACCTGGGTGACGGATATCGATGCCGAAGCCCGTGTGGTGAAAAGCCAGAATAATCAGTGGCAATACGACAAGCTAGTACTGGCAACCGGTGCCAGTGCCTTTGTCCCGCCTGTGCCTGGGCGTGAGTTAATGCTGACGTTAAATAGTCAGCAAGAGTATCGCGCCTGTGAAACGCAACTGCGGGATGCCCGACGCGTGTTGATTGTTGGCGGTGGTTTGATTGGTAGCGAACTGGCGATGGATTTTTGTCGTGCAGGCAAAGCGGTCACGCTAATCGACAACGCTGCCAGTATTCTGGCGTCGTTAATGCCACCGG > NC_000913/2833981‑2834461 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |