Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	284,535	T→G	A445A (GCT→GCG)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	284,535	0	T	G	100.0%	163.1 / NA	57	A445A (GCT→GCG)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (22/35);  total (22/35)

GCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGA  >  NC_000913/284288‑284779                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gcggcgTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACCCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCg                                                                                                                                                                                                                                                   >  1:279184/1‑251 (MQ=255)         ccGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTc                                                                                                                                                                                                                                           <  1:106980/251‑1 (MQ=255)         ccGGAGGTGATGCTCCACCTGCGCGACCGCGGCCTGCTGCATCGGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTTCTTCCCGACGGGCAACATCGCGCCGGATGGTTc                                                                                                                                                                                                                                           <  1:271403/251‑1 (MQ=255)           ggAGGTGATGCTCCACCTGCGCGACCTCGGCCTACTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCgg                                                                                                                                                                                                                                         >  1:133667/1‑251 (MQ=255)            gAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACCTCGCGCCGGAAGGTTCggg                                                                                                                                                                                                                                        >  1:61937/1‑250 (MQ=255)              ggTGATGCTCCCCCTGCGCGACCTCGGCCTGCTGCATCTGGACTCCATGACCGTGACCGGCCAGACGGGGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCTCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCTTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGTCCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGa                                                                                                                                                                                                                                      <  1:263873/251‑1 (MQ=255)              ggTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGa                                                                                                                                                                                                                                      <  1:147424/251‑1 (MQ=255)               gTGATGCACCACCTGCGCGCCCTCGGCTTCCTGTATAAGGACGCCATGACCGCGACCGGACAGACGGCGGGCCAGAACCTTGAACGGTGGCCGGCGACCGAGCGCCGGGCGCGCTTCCGCCCGTACCTGCGCGAGCAGGAAGGCGTCGAGCTGGATACCGTGACCATGCCGCCGGAGAAGGCAAAAGCGAAAGCGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGTAGGCTAGGTGAt                                                                                                                                                                                                                                     >  2:263060/1‑251 (MQ=255)                 gATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATc                                                                                                                                                                                                                                    >  1:115040/1‑250 (MQ=255)                                      cGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGCTGACGTGATCCTGCCGCCGGCGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGt                                                                                                                                                                                                              >  1:281674/1‑251 (MQ=255)                                      cGGCCTGCTGCATCTGGACGCCATGACCGGGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGAccc                                                                                                                                                                                                                <  1:25975/249‑1 (MQ=255)                                       ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTc                                                                                                                                                                                                             <  1:227855/251‑1 (MQ=255)                                       ggCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTc                                                                                                                                                                                                             >  1:255905/1‑251 (MQ=255)                                       ggCCTACTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAAACTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGCGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAACAGCGAAAGGGCGGACCTCGACGGTCTGCTTCCCGACGGACAACATCGCGCCGGAAGGTTCGGGGAGCAAGGACACGGCGATCGACCCGTc                                                                                                                                                                                                             >  2:184087/1‑251 (MQ=255)                                                cATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTggg                                                                                                                                                                                                    <  1:254185/251‑1 (MQ=255)                                                     ggACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAg                                                                                                                                                                                               <  1:274746/251‑1 (MQ=255)                                                                         gcagggcgtggggCGGGACCCTTGAATGGGGGCAGGGGGCGGTGCGCCGGGCGCGCTGCCGCCAGTGGCTGCGCGAGCAGGACGGCGTAGATCCGGATGACGTGATCCTGCTGCCTGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCATCATCGCGCCGGAAGGGTCGGTGATCAAGGCCACGGCGAGCGACCCGTCGTTGGTGGGCGAAGAGGTCGTATACCTGCACAcc                                                                                                                                                                           <  2:121789/241‑1 (MQ=255)                                                                          ccAGCCGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACAccg                                                                                                                                                                          <  1:242195/251‑1 (MQ=255)                                                                            aGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACAccggc                                                                                                                                                                        >  1:234779/1‑251 (MQ=255)                                                                                   gggCGAGAACCTTGAATGGTGCCAGGCGTCCGGGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCTCGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgc                                                                                                                                                                 <  2:53254/251‑1 (MQ=255)                                                                                    ggCGAGAACCTTGACTGGTGGCAGGCGTCCGAGCGCCGGGCGCGTTTCCGCCAATGCCTGCGCGAGCAGGCCGGCGTAGAAACCAATGACGTGATAATGCCGCCGGAAAAGGCAAAAACGAAAGAGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGATCGGTGATCAAGGCAACCGCGCTCGACCCGTCGGAGGAGGGCGAAGATGGCGTATACCACCACACCGGCcgggggcg                                                                                                                                                                >  1:176173/1‑251 (MQ=255)                                                                                      ggagaACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcggg                                                                                                                                                              <  1:78861/250‑1 (MQ=255)                                                                                           acctggaaggggggccggCGTCCGGGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGttt                                                                                                                                                         <  2:45050/235‑1 (MQ=255)                                                                                                        gCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgg                                                                                                                                                               >  1:233095/1‑232 (MQ=255)                                                                                                        gCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCcgggtgcgg                                                                                                                                                               <  2:233095/232‑1 (MQ=255)                                                                                                          aGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACAGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGCCGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAgcgc                                                                                                                                            >  1:113824/1‑249 (MQ=255)                                                                                                                gcGAGGGCCGGGCGCGCTTCGGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGGGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGGCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGGGATCAAGGCCACGGCTATCGACCCGTCGGTGGTGGGCGAAGATGGTGTATACCGCCACTCCGGCCGGGTGCGGGTGTTTGTCTCGTAAGCGCaggcgatc                                                                                                                                    <  2:277024/250‑1 (MQ=255)                                                                                                                     cgcCGGGCGCGCTTCCGCCAGTGCCTGCGCGCGCCGGACGCCGTAGCGCCGGATGCCGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGCCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGCTCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGAGCGGGTGTTTGTCTCGGAAGCGCaggcgatcacggc                                                                                                                               >  1:189626/1‑251 (MQ=255)                                                                                                                      gcCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGCCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGGGCGGGGGTGTGTCTCGGAAGCGCaggcgatcaaggcg                                                                                                                              >  2:260406/1‑251 (MQ=255)                                                                                                                        cGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGACTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCaggcgatcaaggcgat                                                                                                                            <  1:106763/251‑1 (MQ=255)                                                                                                                         gggCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGTAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGGGTTTGTCTAGGAAGAGCaggcgatcaaggcgatc                                                                                                                           >  2:194271/1‑251 (MQ=255)                                                                                                                              ggcTTCCGCCAGGGCCTGCGCGGGCAGGACGGCGTGGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCg                                                                                                                      <  2:53022/250‑1 (MQ=255)                                                                                                                               gcTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCgg                                                                                                                     <  1:275748/251‑1 (MQ=255)                                                                                                                                           gCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATtgtg                                                                                                         >  1:41121/1‑251 (MQ=255)                                                                                                                                               gggagAGCAGGACGGCGTAGGGCCGGATGACCGGGTCCTGCCGCGGGAGAAGGCAAAAGCGAAAGGGCTGACCGCGACGGCCTGCTTCCCGACGGGCAACATCGCGCCGGAAGTTTCGGTTATCAAGGCCACTGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCTTATACCACCACACCGGCCGGGTGCGGGTGTGTGACTCGGAAGCGCAGGCGATCATGGCGATCAATCTGGAAGAGATTGGGCAgg                                                                                                     <  2:37254/247‑1 (MQ=255)                                                                                                                                                        ggACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCa                                                                                            <  1:153832/251‑1 (MQ=255)                                                                                                                                                         gACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCAt                                                                                           <  1:133136/251‑1 (MQ=255)                                                                                                                                                            ggCGTAGAGCCTGATGACGTGCTCCTGCCGCCGGAGAAGGCAACAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGCGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAATCGGGAAGCGATTGTGAAGGGCGCTATCCtggt                                                                                        >  2:134398/1‑251 (MQ=255)                                                                                                                                                               gTAGAGCCGGAGGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTCCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGTTGGGCGAAGATTGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAATCGGTAAGAGATTGTGCAGGGCGATATCATggtggt                                                                                     <  1:153506/251‑1 (MQ=255)                                                                                                                                                                                   ccggccgccGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGCCGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGAGCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                  <  2:183256/247‑1 (MQ=255)                                                                                                                                                                                   ccTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACCTCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTcc                                                                  <  2:21998/250‑1 (MQ=255)                                                                                                                                                                                               gaAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGTGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGAGTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATgg                                                     <  1:274978/251‑1 (MQ=255)                                                                                                                                                                                                       aaGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGACGATGGCGTATACCACCACACCGGCCGGGGGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCACGCGGGACGAGATTGTGCAGGGCGATATCATGGTGGTGGACGTCGGCGGGCAGTCCGGCACAGGCGTGGGAGAGAcc                                             >  2:248199/1‑251 (MQ=255)                                                                                                                                                                                                         gCGAACGGGCTGACCCGGACGGTCGTCTTCCCGACGGGCAACATCGCGCCGGAAGGTGCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGAGCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTa                                           <  2:241400/251‑1 (MQ=255)                                                                                                                                                                                                          cGAAAGGGCTGTCATCGACGGTCTGCCTCCCGGCGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACCCCGGCCGGGTGCGGGTGTTTGTCTCGGGAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGTGCCGTCCGGCACCGGCATGGAAGAGACCTAc                                          <  2:71479/251‑1 (MQ=255)                                                                                                                                                                                                          cGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTggg                                                                                                                                                                                                    <  2:142440/97‑1 (MQ=255)                                                                                                                                                                                                          cGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTggg                                                                                                                                                                                                    >  1:142440/1‑97 (MQ=255)                                                                                                                                                                                                                ggCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTc                                    <  2:113824/251‑1 (MQ=255)                                                                                                                                                                                                                  cTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGGGGGCGAAGATGGCGTATACCACCACACCGGCCGGGAGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGGCCGGCACCGGCAGGGGAGGGACCTACCAGCCCAc                                  >  2:203507/1‑251 (MQ=255)                                                                                                                                                                                                                  cTGACCCCGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGTAGAGACCTACCAGCTCAc                                  >  1:88836/1‑251 (MQ=255)                                                                                                                                                                                                                     cccTCGACGGTCGGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTTCGGTTGTTTGTCGCGTAAGCGCAGGCGATCAAGGCGTTCAATCGGTAAGAGGTTGTGCAGGGCTATATCTTGGGGGTTATCTGCGGCGGGCCGTCCGGCGCCGGGATGTAAGATAGCTATCAGCTCACCTc                               <  2:133667/250‑1 (MQ=255)                                                                                                                                                                                                                         cGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCcg                             <  1:45904/249‑1 (MQ=255)                                                                                                                                                                                                                         cGACGGTCTGCTTCCCGACGGGCAACATCGCGCCGGAAGGTGCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCcgcg                           <  2:210476/251‑1 (MQ=255)                                                                                                                                                                                                                                   cTTCCCGACGGGCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGCCCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAACGCat                  >  1:266382/1‑250 (MQ=255)                                                                                                                                                                                                                                             ggCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTgggg       <  1:272795/251‑1 (MQ=255)                                                                                                                                                                                                                                             ggCAACATCGCGCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTAGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTgggg       <  1:194271/251‑1 (MQ=255)                                                                                                                                                                                                                                                  cATCGCGCCGGAATGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGa  <  2:147543/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GCGGCGTGCCGGAGGTGATGCTCCACCTGCGCGACCTCGGCCTGCTGCATCTGGACGCCATGACCGTGACCGGCCAGACGGTGGGCGAGAACCTTGAATGGTGGCAGGCGTCCGAGCGCCGGGCGCGCTTCCGCCAGTGCCTGCGCGAGCAGGACGGCGTAGAGCCGGATGACGTGATCCTGCCGCCGGAGAAGGCAAAAGCGAAAGGGCTGACCTCGACGGTCTGCTTCCCGACGGGCAACATCGCTCCGGAAGGTTCGGTGATCAAGGCCACGGCGATCGACCCGTCGGTGGTGGGCGAAGATGGCGTATACCACCACACCGGCCGGGTGCGGGTGTTTGTCTCGGAAGCGCAGGCGATCAAGGCGATCAAGCGGGAAGAGATTGTGCAGGGCGATATCATGGTGGTGATCGGCGGCGGGCCGTCCGGCACCGGCATGGAAGAGACCTACCAGCTCACCTCCGCGCTAAAGCATATCTCGTGGGGCAAGA  >  NC_000913/284288‑284779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑