Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,853	C→T	intergenic (‑77/+18)	hda ← / ← uraA	inibitor of reinitiation of DNA replication/uracil:H(+) symporter UraA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,853	0	C	T	100.0%	66.1 / NA	21	intergenic (‑77/+18)	hda/uraA	inibitor of reinitiation of DNA replication/uracil:H(+) symporter UraA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/8);  total (13/8)

CGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCC  >  NC_000913/2618625‑2619087                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           cgTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACattatt                                                                                                                                                                                                                      >  2:143919/1‑251 (MQ=255)                atGTAACAGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGACGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCCGCAATACGGCCCGGTCGGTACATTATTTGTCTGTTATATCcg                                                                                                                                                                                                       >  1:305938/1‑251 (MQ=255)                                       cagcaCGTTTTGCAGCGCGGCCAGTCAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATACAGTGGCCAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTAACACAGAATCCCATGATGTTTGAAACGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCAct                                                                                                                                                                                >  2:311251/1‑251 (MQ=255)                                                   cAGCGCGGCCAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGgcgc                                                                                                                                                                    >  2:112100/1‑251 (MQ=255)                                                            cAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGTGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTg                                                                                                                                                           >  1:12419/1‑251 (MQ=255)                                                              ggaaaGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCGCAACCAGGATTTCACAAAATCGCTAGAAGTTTACCACAGAATCCCAGGATTTTTGAACCTGGCAGCAATACGGCCTGGGCGGTGCATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCGCCACTTCTTCAGGGCGCAGCACACTGag                                                                                                                                                         <  2:253102/249‑2 (MQ=255)                                                                     ggagTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCCCCACTGCTTCAGGGCGCAGCTCACTGATCAGTTTg                                                                                                                                                  <  2:47162/251‑1 (MQ=255)                                                                                         aaCTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCATGATTTCACAAAATCGCGAGAAGTTTACCACAGAATTCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGAt                                                                                                                              <  1:120632/251‑1 (MQ=255)                                                                                         aaCTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGAt                                                                                                                              <  1:191993/251‑1 (MQ=255)                                                                                           cTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATAc                                                                                                                            >  1:207585/1‑251 (MQ=255)                                                                                                                                                      cAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGCAGTTTACCACAGAATCCCATGATGTTTGAAACGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTcgcg                                                                 >  1:310008/1‑251 (MQ=255)                                                                                                                                                           aTACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACt                                                            >  1:240802/1‑251 (MQ=255)                                                                                                                                                             aCCTCAACCAGGATTTCCCAAAATCGCGAGTAGTTTCCCCCAGAATCCCATGATGTTTGAACCGGGCTGCATTCCTGCCCGGTCGGTTCATTATTTGTCTGTTATTTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAATATCAAACTTAAGCCGATACCGTCGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTg                                                           <  2:53224/250‑1 (MQ=255)                                                                                                                                                               cTCAACCAGGATTTCACAAAATCGCGAGAAGTTTAACACAGAAACCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATAAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGATTCCCTTCGCGCCACTGACg                                                        >  1:48466/1‑251 (MQ=255)                                                                                                                                                                 cAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGcc                                                      <  2:193939/251‑1 (MQ=255)                                                                                                                                                                           ttaacaAAATCGCGAGAAGTTTACCACAGCATTCCATGATGTTTGAACCAGGCAGCAATACTGCCCGGTCGGTCCATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCAAACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCACCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTATACCTTCGCGCCACTGACGCCGATGATCaa                                             >  2:203716/4‑250 (MQ=255)                                                                                                                                                                                            aGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCGTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGAt                           <  1:112100/251‑1 (MQ=255)                                                                                                                                                                                              tttACCACAGAATCCCATGATGTTTGAACCGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATc                          >  2:69458/1‑250 (MQ=255)                                                                                                                                                                                                tACCACAGAATCCCATGATGTTTGAACCGGGCAGCCTTAATGCCCGGTAGCTACATCCTTTGTCTGTTATATCCGCGTCTACTGCGTCCAGGACCACTTATTCAGGGCGCAGCCAACTGATCAGCTTGAAGATCAGACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTATTAACTCCGCCGCGCCGATGTTTCCCTTCGCGCCACTGACGCCGATCATCAAAATCACGGAAGTCAGGATCAgg                       >  1:310911/1‑251 (MQ=255)                                                                                                                                                                                                                      tgacccGGGCAGCAATACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCGGCGTCCAGCCCCCCTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATcc  <  2:12419/246‑1 (MQ=255)                                                                                                                                                                                                                               cagcaATACTGCCCGGTCGGAACATTATTTGTCTGTTATTTCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTc                                                                                          >  2:219719/1‑153 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           CGTGCCCAGAGATAGATGTAACCGCTATGTTCCTGACGCAGCACGTTTTGCAGCGCGGCCAGTAAAGAGGAGTTATCCCCCGGCCAGAAACTTGCAAAGGTTTCGTCGTCAGGAAGATAAAGTGGCAAAGAGAGCTGTGCCGGTGTGTTCAGAGATACCTCAACCAGGATTTCACAAAATCGCGAGAAGTTTACCACAGAATCCCATGATGTTTGAACCGGGCAGCAACACTGCCCGGTCGGTACATTATTTGTCTGTTATATCCGCGTCTTCTGCGTCCAGCACCACTTCTTCAGGGCGCAGCACACTGATCAGTTTGAAGATCAAACTTAAGCCGATACCGACGATGGTCGCCAGCGCCATACCTTTTAACTCCGCCGCGCCGATGTTTACCTTCGCGCCACTGACGCCGATGATCAAAATCACGGAAGTCAGGATCAGGTTCTGTGCTTTGTTGTAATCC  >  NC_000913/2618625‑2619087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑