Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,587,112	C→T	A461V (GCA→GTA)	narQ →	sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,587,112	0	C	T	96.5%	88.2 / NA	29	A461V (GCA→GTA)	narQ	sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/13);  minor base G (1/0);  total (16/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGG  >  NC_000913/2586871‑2587340                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     cGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATgc                                                                                                                                                                                                                             >  1:97001/1‑251 (MQ=255)  gTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATgcg                                                                                                                                                                                                                            >  1:193123/1‑251 (MQ=255)       cAGTTGACGTTACTGAAGCGTGCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTTTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGAGCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTTACCCTCGACTGCCGTCTGCCAACCCGGGTACTGGATGCGCAAAt                                                                                                                                                                                                                       <  2:191979/251‑1 (MQ=255)            gACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGAGCCTCGACTGCCTTGTGCCAACCCTGGTACTGGATGCGCAAATGCAgg                                                                                                                                                                                                                  <  1:112150/251‑1 (MQ=255)                                      aaGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCCTTGAGGGCAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGGCTGCCGTCTGCCAACCCTGGGACTGGATGCGCAAAAGCCGGTGCATTTGTTGCAAATTATTCGCGa                                                                                                                                                                                         >  2:134494/1‑250 (MQ=255)                                             cGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGTCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGc                                                                                                                                                                                 <  2:20906/251‑1 (MQ=255)                                                                aTGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGGCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCacgc                                                                                                                                                              <  1:129172/251‑1 (MQ=255)                                                                                    gTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGTAGCACGCCAACGCCAGCGTAATCGCCg                                                                                                                                          >  1:233012/1‑251 (MQ=255)                                                                                         atgatgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTGCCGCCTGACTCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTTGTACTGGATTCGCAAATGCAGTTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAATCACGCCAACGCCAGCGAAATCGCCGTCAGt                                                                                                                                     <  2:49024/251‑1 (MQ=255)                                                                                            atgCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCCAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGc                                                                                                                                  >  1:295830/1‑251 (MQ=255)                                                                                                ttATCGGCAGTTTCGCCAGCTGTTGACTACATTCCGACTGACCCTCCAGCAGGCGGCTCTCACCTCCGCCTTGAGGGAAATGCTGGATACGTTACAAAATCACACCCGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAACGGTGCTGAATGCGGTGAAGCACGCCCACGCCCGCGAAATCGCCGTCAGTTGCGTc                                                                                                                               >  2:298875/1‑250 (MQ=255)                                                                                                ttATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAACTGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGCAATCGCCGTCAGTTGCGTc                                                                                                                               >  1:45944/1‑250 (MQ=255)                                                                                                        aGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCCCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCTAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCg                                                                                                                      <  1:316401/251‑1 (MQ=255)                                                                                                              gcgAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGc                                                                                                                >  1:283469/1‑251 (MQ=255)                                                                                                                     gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGTGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCCAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGAAGCCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATcccc                                                                                                         >  2:71480/1‑248 (MQ=255)                                                                                                                     gTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGTTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATGCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAAt                                                                                                             <  1:44518/247‑1 (MQ=255)                                                                                                                        gACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACg                                                                                                       <  1:29823/250‑1 (MQ=255)                                                                                                                                       gACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGAt                                                                                        >  1:177062/1‑250 (MQ=255)                                                                                                                                              cagcagGCGGATCTCCCCTCCGCATTGAGGGAAAGGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCATCCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCGATCACACGGTTTATATCCGTGATAACGGGAt                                                                                <  1:182926/251‑1 (MQ=255)                                                                                                                                                              ccTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCTAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCg                                                                <  2:177062/251‑1 (MQ=255)                                                                                                                                                                           ggAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGa                                                     >  1:314302/1‑249 (MQ=255)                                                                                                                                                                             aaaTGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGGTTATATCCGTGATAACGGGATTGGTATCGGTGAGCCGAAAGAACCCGAAGGt                                                 <  2:193123/251‑1 (MQ=255)                                                                                                                                                                                          gTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTAATGGATGCGCAAATGCAGGTGCATTTGCTGCAAATTATTCGCGAAGCGGTGCTGAATGCGCTGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTCCGTCACCGCGCCGGACGGCCATCACACGGTTTATATCCGTGATAACGGGATGGGTATCTGCGAACCGAAGGAACCCGCCGGTCATTATGGGCTg                                     >  2:76851/1‑250 (MQ=255)                                                                                                                                                                                           ttACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGa                                    >  2:242788/1‑250 (MQ=255)                                                                                                                                                                                                    cAAACCAGCGCAAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGCCGGCAATCACACGGTTTATATCCGGGATAACGGGATTGGTATCGGTGAACCGAAAGAACACGAAGGTCATTATGGTCTGAATATCATgcg                          >  2:276267/1‑251 (MQ=255)                                                                                                                                                                                                          aGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATTGTCTGAATATCATGCACGAAcg                    <  1:88814/251‑1 (MQ=255)                                                                                                                                                                                                                 aaCTGACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGa              >  2:920/1‑250 (MQ=255)                                                                                                                                                                                                                     gACCCTCGACTGCCGTCTGCCAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGc         >  1:180250/1‑251 (MQ=255)                                                                                                                                                                                                                            gACTGCCGTCTGACAACCCTGGTACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCAGTGCTGAATGCGAAGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAGCCCGAAGGTCATTATGGTCAGAATATCATGCGCGAACGCGCGGAACGGCTAGGtag  <  2:233012/251‑3 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     CGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAAGTATCATGGCCGATTTTTCCCAGGCGTTGAATGATGCTTATCGGCAGTTACGCGAGCTGTTGACTACTTTCCGCCTGACGCTGCAGCAGGCGGATCTCCCCTCCGCATTGAGGGAAATGCTGGATACGTTACAAAATCAAACCAGCGCCAAACTGACCCTCGACTGCCGTCTGCCAACCCTGGCACTGGATGCGCAAATGCAGGTGCATTTGTTGCAAATTATTCGCGAAGCGGTGCTGAATGCGATGAAGCACGCCAACGCCAGCGAAATCGCCGTCAGTTGCGTCACCGCGCCGGACGGCAATCACACGGTTTATATCCGTGATAACGGGATTGGTATCGGTGAACCGAAAGAACCCGAAGGTCATTATGGTCTGAATATCATGCGCGAACGCGCGGAACGGCTAGGTGG  >  NC_000913/2586871‑2587340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑