Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,738	T→C	E476E (GAA→GAG)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,738	0	T	C	98.4%	198.6 / NA	61	E476E (GAA→GAG)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (27/33);  minor base A (1/0);  total (28/33)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

GTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAC  >  NC_000913/2398500‑2398970                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         gTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCa                                                                                                                                                                                                                              <  2:224831/251‑1 (MQ=255) gTTCGGATAAAGCTTCTTCAAACGAACCGCAACACATCATGCCCAGCCCTCTGCAGTTGAC‑GGAAAAGGCAATCATGCAGATACCGACGTCAGCGACGCGACCGTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCAGGAGATAATCAACGGTTTCTTAGCACCTGCCAGTGCCTGCACGATGACGTCGCTTTTGCTTTGCACCTCCGGCTCGATACAGTc                                                                                                                                                                                                                               >  2:199275/1‑250 (MQ=255)    cGGTTAACGCTTCTTCAAGCCAACCGCCTCCCATAATGCCCAGCCCCATGCTGTTTAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGTGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCATGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAAcc                                                                                                                                                                                                                           <  2:169078/251‑1 (MQ=255)     ggTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGCCGCTTTTGCTTTGCAGCTCAGGCTCGATACCGTCAAccc                                                                                                                                                                                                                          >  2:10307/1‑250 (MQ=255)         accGCTTCTTCAAGCGAACCTCCACCCATAATGCCCCGCCCCATGCTGT‑GACCGGAACGGGCAATCATGGTGATACCGCCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTg                                                                                                                                                                                                                       <  2:70123/248‑1 (MQ=255)         aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTgg                                                                                                                                                                                                                      >  2:238318/1‑251 (MQ=255)                       cGAACCGCAACCCATAATGCCCAGCTCCATGCTGTTGAC‑GGACCGGGCAATCCTGGTGCTACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTCGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGCTAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATccc                                                                                                                                                                                                        >  2:142468/1‑250 (MQ=255)                           ccGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCCTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc                                                                                                                                                                                                    >  2:44850/1‑251 (MQ=255)                            cGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc                                                                                                                                                                                                    >  2:280192/1‑250 (MQ=255)                             gCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAt                                                                                                                                                                                                  >  1:221855/1‑251 (MQ=255)                             gCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGAGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGATCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAt                                                                                                                                                                                                  >  1:291590/1‑251 (MQ=255)                                       aTGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGc                                                                                                                                                                                        <  1:218951/251‑1 (MQ=255)                                                    tGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAAcgc                                                                                                                                                                           >  1:226404/1‑251 (MQ=255)                                                               ggAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGCTACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCtt                                                                                                                                                                 >  2:12881/1‑251 (MQ=255)                                                                 aaCGGGCAATCATGGTGCAACCGTCGCCAGCGCCGCGACCTTGCAGGGCGTTCGCGACGTTAGCTGCCGCCTGAATCACCTCAAAGCTCCCGGCGTGCGTCCCGGAGATAATTATCGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCGAAACGCGCCTGATCTTCa                                                                                                                                                               <  2:233904/251‑1 (MQ=255)                                                                             tGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgc                                                                                                                                                                                                      >  2:140830/1‑200 (MQ=255)                                                                             tGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgc                                                                                                                                                                                                      <  1:140830/200‑1 (MQ=255)                                                                                     ccGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGCCGTTAGACGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTcc                                                                                                                                            >  1:30636/1‑250 (MQ=255)                                                                                         cGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgcc                                                                                                                                       <  2:221855/251‑1 (MQ=255)                                                                                           tCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCATCTGCCAGTGCCTTCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgccg                                                                                                                                      <  1:205942/250‑1 (MQ=255)                                                                                                gccgcGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGatat                                                                                                                                >  1:262715/1‑251 (MQ=255)                                                                                                   gcgACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatcat                                                                                                                             <  1:292028/251‑1 (MQ=255)                                                                                                    cgACCTTTCAGGGCTGACGCGACGATAGCTGACGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCACCGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGCGTTATCCAGCTCATGGGCGATGGCAAAACCTAAACGAGCCTGATCTTCAACCGATGCGCGGTAAGTCCACGCCTCGATatcatc                                                                                                                            >  2:16630/1‑251 (MQ=255)                                                                                                     gACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATcc                                                                                                                           >  1:22501/1‑251 (MQ=255)                                                                                                              gggCTTCCGCGACGTTAGCTGCCGCCTGAATCCCCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGgtg                                                                                                                  <  2:141339/251‑1 (MQ=255)                                                                                                                       cgACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCTCCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACg                                                                                                         <  1:10307/251‑1 (MQ=255)                                                                                                                               gctgccgccTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTaac                                                                                                 <  1:280192/248‑1 (MQ=255)                                                                                                                                  gccgccGGAATCACCTCTAGGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTaacaaa                                                                                              <  1:80971/251‑1 (MQ=255)                                                                                                                                     gccttaagccccTCTAAGCTACCGGCGTCCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCACCCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAg                                                                                           <  2:280420/241‑1 (MQ=255)                                                                                                                                         gAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCgg                                                                                        <  1:112764/250‑1 (MQ=255)                                                                                                                                           atccccTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGTTTTCTGCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATAGCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATg                                                                                     <  2:262715/247‑1 (MQ=255)                                                                                                                                             cACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATAAACGTTTTCTTCGCACCTGCCCGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGATCAGGCTCGATACCGGCAACCGCTGGTGCAGAGTTATCCAGCGTATGGGCGATGGCAAAACCTAAGCGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGAGATCATCCAGACGGGTGTCATCAACGTTGGTAACAAGCAGCGGATGCt                                                                                   <  2:19353/251‑1 (MQ=255)                                                                                                                                                   tAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCAc                                                                             >  1:135897/1‑251 (MQ=255)                                                                                                                                                    aaGCTACCGGCGTTCGTCCCGGAGATAATCCACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTAATCAACGTTGGTAACAAACAGCGGATGCTTCGCACg                                                                            >  2:189946/1‑251 (MQ=255)                                                                                                                                                       cTACCGGCGTTCGTCACGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTg                                                                         >  1:80576/1‑251 (MQ=255)                                                                                                                                                          ccGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCtt                                                                                  >  2:244061/1‑239 (MQ=255)                                                                                                                                                          ccGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCtt                                                                                  <  1:244061/239‑1 (MQ=255)                                                                                                                                                            ggCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCg                                                                     >  1:135739/1‑250 (MQ=255)                                                                                                                                                              cGTTCGCCCCGGAGATAATCAACGGTTTCTCCGCCCCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATg                                                                  <  2:235159/251‑1 (MQ=255)                                                                                                                                                               ggtCGTCCCGGATATAATCAACGGTTTCTTAGGCCCGGCCAGTGCCGGCACGATGACGTCAATTTTGCGTTGCAGCCCAGTCGCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAATTCCACGCCGCGATATCATCCAGGCGGGTGTCATCAACGTTTGTAACAAACAGCGGATGCTTCGCACGTTGACCGTTGt                                                                 <  2:65806/249‑1 (MQ=255)                                                                                                                                                               gTTCGCCCCGTAGATAAGCAACGGTTTCTTCGCCCCTGCCAGGGCCTGCGCGATGACGTCGATTTTGCTTTGCAGCGCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGTCAAATCCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGTGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGt                                                                 <  1:298873/251‑1 (MQ=255)                                                                                                                                                                       cGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGAtt                                                         >  2:36803/1‑251 (MQ=255)                                                                                                                                                                        ggAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTg                                                        <  1:254435/251‑1 (MQ=255)                                                                                                                                                                          ggaTAATCAACGGTTTCTTCGCACCTGCCAGGGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGGTGGTAACAAACAGCGGATGCTTCGCTCGTTTACGTGTGTTGAGGATTGcc                                                      <  2:30636/250‑1 (MQ=255)                                                                                                                                                                               cccgaccctcttccgatctcTGCCAGTGCCGGCCCGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGAGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAAt                                                 <  2:162939/232‑1 (MQ=255)                                                                                                                                                                                 cAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTc                                                                                                                                                                >  1:23260/1‑138 (MQ=255)                                                                                                                                                                                 cAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTc                                                                                                                                                                <  2:23260/138‑1 (MQ=255)                                                                                                                                                                                    cGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGcc                                            <  1:206960/251‑1 (MQ=255)                                                                                                                                                                                    cGGTTTCTACGCACCTGCCAGTGCCTGCACGATGCCGTCTATTGTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAGGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATAGCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGTATGCGTCGCACGTTGACCGATGTTTAGGATTGCCGCAATCTGcc                                            <  2:158425/251‑1 (MQ=255)                                                                                                                                                                                       tttCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGCTACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGt                                         >  2:108171/1‑251 (MQ=255)                                                                                                                                                                                            tcGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAGCGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCa                                       <  2:226404/248‑1 (MQ=255)                                                                                                                                                                                                  cTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAAt                                                 >  1:162939/1‑232 (MQ=255)                                                                                                                                                                                                     ccAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGTCGAGGGCAAAACCTAAACGCGCCTGATCTTCAACCTTTGCGCGGTAAGTCCGCGCCGCGATATCATCCAGACGGGTGGCATCAACGTTGGTAACAAACAGCGGAGGCTTCGCGCGTGTACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCtgt                           <  1:33079/251‑1 (MQ=255)                                                                                                                                                                                                       aGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACACCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGtgc                         >  2:226805/1‑251 (MQ=255)                                                                                                                                                                                                           ccTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTgcc                     >  1:166415/1‑251 (MQ=255)                                                                                                                                                                                                                aCGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAt                <  1:74770/251‑1 (MQ=255)                                                                                                                                                                                                                  gATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCAttt              >  1:208028/1‑251 (MQ=255)                                                                                                                                                                                                                     gACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCCGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGCTATCATCCAGACGGGTGTCATCAACGTTGGTAACACACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCGGCCAGTCACCCACTTTCTGTGCTGCCGCCATTTctc           >  2:241014/1‑249 (MQ=255)                                                                                                                                                                                                                      aCGTCGTTTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGATGGTGCTGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAACCGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACCGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTcgcg          <  2:166938/251‑1 (MQ=255)                                                                                                                                                                                                                          cGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAATCCTAAACGCGCGTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCGTCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCt      <  1:57145/251‑1 (MQ=255)                                                                                                                                                                                                                               ttgctttgcAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAc  <  1:36803/250‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                         GTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACGTTGGTAACAAACAGCGGATGCTTCGCACGTTGACCGATGTTGAGGATTGCCGCAATCTGCCAGTCAGCCACTTTCTGTGCTGCCGCCATTTCGCGCGCTTTAC  >  NC_000913/2398500‑2398970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑