Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,398,627	C→T	A513A (GCG→GCA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,398,627	0	C	T	97.9%	145.3 / NA	47	A513A (GCG→GCA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (28/18);  total (29/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

CCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACG  >  NC_000913/2398389‑2398867                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ccaccaTCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATc                                                                                                                                                                                                                                      <  1:314670/251‑1 (MQ=255)          ccAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTGCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAg                                                                                                                                                                                                                             <  1:125083/251‑1 (MQ=255)                 tGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCgg                                                                                                                                                                                                                      >  1:175470/1‑251 (MQ=255)                      gaGCCAGAGCAGAAATCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTGCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCAGGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCGTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGtt                                                                                                                                                                                                                  <  2:122925/249‑1 (MQ=255)                        gCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGt                                                                                                                                                                                                               >  1:158425/1‑251 (MQ=255)                        gCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGt                                                                                                                                                                                                               >  2:206960/1‑251 (MQ=255)                                        cgcgGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAAc                                                                                                                                                                                               >  2:65812/1‑251 (MQ=255)                                              tAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGGTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATACCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGAGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGCttc                                                                                                                                                                                         >  1:229270/1‑251 (MQ=255)                                                gcaggggagggtcGATGCAGATCGTTTGCCAACACCCCCACCGCGTCGGCGCGTCCGGTTTCCTGTTCGGTTAACTCCTCTTCAAGCGAACCGCCCCCCATAATGCCCAGCCACATGCTGTTGAC‑GGAACGGGCAATCATGGTGATTCCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCTCCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTttctt                                                                                                                                                                                       <  2:229270/239‑1 (MQ=255)                                                gCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTttctt                                                                                                                                                                                       >  1:235159/1‑251 (MQ=255)                                                 cAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCACGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAAGCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTttcttc                                                                                                                                                                                      <  1:286375/251‑1 (MQ=255)                                                           aCGATGCAGATCGTTTTCCAACACCACCACCGCGTCGTCGCGGCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACACATAATGACCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCCGCGCCGCGGCCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCACGGAGATAATCAACGGTTTCTTCGCCCCTGcc                                                                                                                                                                             >  2:53276/1‑250 (MQ=255)                                                             gATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAAAGGTTTCTTCGCACCTGCCAGt                                                                                                                                                                          >  2:292028/1‑251 (MQ=255)                                                                gCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGcc                                                                                                                                                                       <  2:71692/251‑1 (MQ=255)                                                                  aGATCGTTTTCCAACACCCCCACCGCGTCGGCGCGTCCGGTTTACAGTTCGGTTAACGCTTCTTCAAGAGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGCGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGACGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATACTCACCGGTTTCTTCGCACCTTCCAGTGCCTg                                                                                                                                                                     >  1:281286/1‑251 (MQ=255)                                                                    aTCGTTTTCCAACACCACCACCGCGCCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAGGCCACCGGCGTTCGTCCCGGAGCTAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGc                                                                                                                                                                    >  2:206003/1‑250 (MQ=255)                                                                        ttttCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCTGAGATAATCAACGGGTTCTTCGCACCTGCCAGTGCCTGCACGAt                                                                                                                                                               >  2:74770/1‑251 (MQ=255)                                                                           tCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGAc                                                                                                                                                            >  1:173362/1‑251 (MQ=255)                                                                                   caccaccacGTCGGCGCGTCCGGTTTCCAGTTCGGTTCACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCATCGGTTTCTTCGCACCTCCCAGTGCCTTCACGCTGACGACGAttt                                                                                                                                                    >  2:80428/1‑251 (MQ=255)                                                                                                   cgTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAgg                                                                                                                                    >  1:141339/1‑251 (MQ=255)                                                                                                   cgTCCGGTTTCCAGTCCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAgg                                                                                                                                    <  2:10218/251‑1 (MQ=255)                                                                                                                gTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCa                                                                                                                       <  2:224831/251‑1 (MQ=255)                                                                                                                gTTCGGATAAAGCTTCTTCAAACGAACCGCAACACATCATGCCCAGCCCTCTGCAGTTGAC‑GGAAAAGGCAATCATGCAGATACCGACGTCAGCGACGCGACCGTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCAGGAGATAATCAACGGTTTCTTAGCACCTGCCAGTGCCTGCACGATGACGTCGCTTTTGCTTTGCACCTCCGGCTCGATACAGTc                                                                                                                        >  2:199275/1‑250 (MQ=255)                                                                                                                   cGGTTAACGCTTCTTCAAGCCAACCGCCTCCCATAATGCCCAGCCCCATGCTGTTTAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGTGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCATGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAAcc                                                                                                                    <  2:169078/251‑1 (MQ=255)                                                                                                                    ggTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGCCGCTTTTGCTTTGCAGCTCAGGCTCGATACCGTCAAccc                                                                                                                   >  2:10307/1‑250 (MQ=255)                                                                                                                        accGCTTCTTCAAGCGAACCTCCACCCATAATGCCCCGCCCCATGCTGT‑GACCGGAACGGGCAATCATGGTGATACCGCCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTg                                                                                                                <  2:70123/248‑1 (MQ=255)                                                                                                                        aaCGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTgg                                                                                                               >  2:238318/1‑251 (MQ=255)                                                                                                                                      cGAACCGCAACCCATAATGCCCAGCTCCATGCTGTTGAC‑GGACCGGGCAATCCTGGTGCTACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTCGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGCTAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATccc                                                                                                 >  2:142468/1‑250 (MQ=255)                                                                                                                                          ccGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCCTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc                                                                                             >  2:44850/1‑251 (MQ=255)                                                                                                                                           cGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgcgc                                                                                             >  2:280192/1‑250 (MQ=255)                                                                                                                                            gCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAt                                                                                           >  1:221855/1‑251 (MQ=255)                                                                                                                                            gCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGAGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGATCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAt                                                                                           >  1:291590/1‑251 (MQ=255)                                                                                                                                                      aTGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGc                                                                                 <  1:218951/251‑1 (MQ=255)                                                                                                                                                                   tGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAAcgc                                                                    >  1:226404/1‑251 (MQ=255)                                                                                                                                                                              ggAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGCTACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCtt                                                          >  2:12881/1‑251 (MQ=255)                                                                                                                                                                                aaCGGGCAATCATGGTGCAACCGTCGCCAGCGCCGCGACCTTGCAGGGCGTTCGCGACGTTAGCTGCCGCCTGAATCACCTCAAAGCTCCCGGCGTGCGTCCCGGAGATAATTATCGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCGAAACGCGCCTGATCTTCa                                                        <  2:233904/251‑1 (MQ=255)                                                                                                                                                                                            tGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgc                                                                                               <  1:140830/200‑1 (MQ=255)                                                                                                                                                                                            tGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAgc                                                                                               >  2:140830/1‑200 (MQ=255)                                                                                                                                                                                                    ccGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGCCGTTAGACGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTcc                                     >  1:30636/1‑250 (MQ=255)                                                                                                                                                                                                        cGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgcc                                <  2:221855/251‑1 (MQ=255)                                                                                                                                                                                                          tCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCATCTGCCAGTGCCTTCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgccg                               <  1:205942/250‑1 (MQ=255)                                                                                                                                                                                                               gccgcGACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGatat                         >  1:262715/1‑251 (MQ=255)                                                                                                                                                                                                                  gcgACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatcat                      <  1:292028/251‑1 (MQ=255)                                                                                                                                                                                                                   cgACCTTTCAGGGCTGACGCGACGATAGCTGACGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCACCGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGCGTTATCCAGCTCATGGGCGATGGCAAAACCTAAACGAGCCTGATCTTCAACCGATGCGCGGTAAGTCCACGCCTCGATatcatc                     >  2:16630/1‑251 (MQ=255)                                                                                                                                                                                                                    gACCTTTCAGGGCTTTCGCGACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATcc                    >  1:22501/1‑251 (MQ=255)                                                                                                                                                                                                                             gggCTTCCGCGACGTTAGCTGCCGCCTGAATCCCCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGgtg           <  2:141339/251‑1 (MQ=255)                                                                                                                                                                                                                                      cgACGTTAGCTGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCTCCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGCTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACg  <  1:10307/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 CCACCATCACCAGCGGTGCTTTAGCCAGCGCAGCATTCACGCGGATAGCAGAAGCGTGACGATGCAGATCGTTTTCCAACACCACCACCGCGTCGGCGCGTCCGGTTTCCAGTTCGGTTAACGCTTCTTCAAGCGAACCGCCACCCATAATGCCCAGCCCCATGCTGTTGAC‑GGAACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTCATCAACG  >  NC_000913/2398389‑2398867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑