| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,333,109 | G→A | intergenic (‑3312/+1227) | yfaQ ← / ← yfaT | tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,333,109 | 0 | G | A | 95.7% | 68.0 / ‑3.9 | 23 | intergenic (‑3312/+1227) | yfaQ/yfaT | tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/13); minor base . (0/1); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACTTTAACATCGACAC > NC_000913/2332866‑2333327 | tGCCTTCCAGCGACACGGGGAAACGTCCGGCATCACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTACCTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCAGCTATAGACCTAATTG‑Cgg > 1:156168/1‑251 (MQ=255) cATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTCTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTATTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCt < 2:168568/251‑1 (MQ=255) aaTCGTTACCGACCATTGATACTTGCTGAGCGCGCAAACTTAACTGCACGCGGGCCTTTTTTCCCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGACAACTGCTGCTATAGACCTAATTG‑CGGTAAGCACGACGTAACTCATAACCTCCCGCTACGGCAttt > 2:129174/1‑251 (MQ=255) ttACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTgg > 1:220724/1‑251 (MQ=255) tGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGCTGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGCAGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGAGGAAACGTCCCTg > 2:260782/1‑251 (MQ=255) gAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGGTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTTCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCTTCTACGGCAGTTTCTGGCATGTGGAAACTCCCCTGcc < 2:313660/249‑1 (MQ=255) gCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCATTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGc < 1:129174/251‑1 (MQ=255) cctctgggCATTTTTTCCCGGCTCGCCAGCCGGGTATAGTAGTGGCAGTTTGCCGCTGACCGCTTCGCGATTTTTGAACTCTTGTTTGGCGAGAGTTAAACCAATCTCGAAAGGTGGCTTGCTGGTGTTTGACACGCGAAAACTGCTGCTATAGACTTAGTTG‑TGGTTACCAAGACGTATCTCATATCCTCCGGCGACGGCATTTTCTGGCAGGCGGAAACTTCCCTGGCCGCCATTGCGCGCATCCAGCg < 2:125394/246‑1 (MQ=255) cgcgGGCTTTTTTTACCGGCTCGCCATCCGGGTAGAGCTGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAATACGTAACTCATAACCCCCGGCTACGTCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTg < 1:76749/251‑1 (MQ=255) cGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGGCCTAATTG‑CGGTAAGCAAGACGTAACTCAGAACCCCCGGCTACTGCATTTTCTGGCAGGCGTAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGGTTGCa < 1:252424/251‑1 (MQ=255) agccGGGTTGTGCAGTTGCAGTTTGCCGGTGACCGTTTCGCCGTTTTTGACCTCTTTTTTGTCGAGAGCTAAACCAATCCCGAAATGTGGCTTGATGGAGTTTGCCCCGCGAAAACTGCTGCTAGAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATACCCTCCGGATACGGCATTTCCTGGAAGGCGAAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGc < 2:269311/249‑1 (MQ=255) aTCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATaa > 1:251199/1‑160 (MQ=255) aTCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATaa < 2:251199/160‑1 (MQ=255) gcagttCGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCAcc < 1:268593/251‑1 (MQ=255) cGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGCAAGCTTCGCCgggg > 2:231924/1‑251 (MQ=255) ccAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGAc < 1:92209/251‑1 (MQ=255) cAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTCGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACg > 1:179515/1‑251 (MQ=255) gTTTTGAACTCTTTTTTGGGGAGAGCTAAACCAATCTCGAAATGTGGCTGGATGGAGTTTGCCACGCGAAAACTGCTGCTATAGCCCT‑ATTGGCGGTAAGGAAGACGTAACCCATATCCTCCGGCTTCGGCATTTTCTGGCAGGCTGAAACTGCCCTGCTCGCCATTGCGCGCATCCAGCTTGACATTGACGGTTTGCAACAGACTTCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGAt < 1:254552/251‑1 (MQ=255) ctTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCtt < 1:42411/223‑1 (MQ=255) ctTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCtt > 2:42411/1‑223 (MQ=255) ggCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAAAGCAACg < 1:89139/251‑1 (MQ=255) gTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACTTTAACATCGa < 1:231924/251‑1 (MQ=255) gTTTGCCACGCGAAAACTGCTGCTATAGACCTAATTG‑CGGTATGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAAGCGGAAACTTCCCTGCCCTCCATTGCACGAATCTAGCGTGACTTTGACGTGTTGCAACACACTGCCGGTGGCTGCCAGCAACGAAAACTTCGCCGGGGCGCTGACGATGGGGGATGACTGCAACCGATTGTTGAACTCGCTGCCGGTCACTTTATAATCGacac > 2:74029/1‑251 (MQ=255) | TGCCTTCCAGCGACACGGGGAAACGTCCGGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTG‑CGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACTTTAACATCGACAC > NC_000913/2332866‑2333327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |